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Abstract:

This study was conducted to investigate whether functions encoded in the metagenome could improve our ability to understand the link between microbial community structures and functions in activated sludge. By analyzing data sets from six industrial and six municipal wastewater treatment plants (WWTPs), covering different configurations, operational conditions, and geographic regions, we found that wastewater influent composition was an overriding factor shaping the metagenomic composition of the activated sludge samples. Community GC content profiles were conserved within treatment plants on a time scale of years and between treatment plants with similar influent wastewater types. Interestingly, GC contents of the represented phyla covaried with the average GC contents of the corresponding WWTP metagenome. This suggests that the factors influencing nucleotide composition act similarly across taxa and thus the variation in nucleotide contents is driven by environmental differences between WWTPs. While taxonomic richness and functional richness were correlated, shotgun metagenomics complemented taxon-based analyses in the task of classifying microbial communities involved in wastewater treatment systems. The observed taxonomic dissimilarity between full-scale WWTPs receiving influent types with varied compositions, as well as the inferred taxonomic and functional assignment of recovered genomes from each metagenome, were consistent with underlying differences in the abundance of distinctive sets of functional categories. These conclusions were robust with respect to plant configuration, operational and environmental conditions, and even differences in laboratory protocols. © 2016, American Society for Microbiology.

Registro:

Documento: Artículo
Título:Shotgun metagenomic profiles have a high capacity to discriminate samples of activated sludge according to wastewater type
Autor:Ibarbalz, F.M.; Orellana, E.; Figuerola, E.L.M.; Erijman, L.
Filiación:Instituto de Investigaciones en Ingeniería Genetica y Biologia Molecular Dr. Hector N. Torres, INGEBI-CONICET, Buenos Aires, Argentina
Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Activated sludge process; Industrial plants; Industrial water treatment; Microorganisms; Nucleotides; Environmental conditions; Environmental difference; Microbial communities; Microbial community structures; Municipal wastewater treatment plants; Nucleotide composition; Operational conditions; Wastewater treatment system; Wastewater treatment; abundance; activated sludge; community structure; data set; genomics; microbial community; taxonomy; wastewater; sewage; waste water; bacterium; bioremediation; chemistry; classification; genetics; isolation and purification; metabolism; metagenome; metagenomics; microbiology; phylogeny; procedures; sewage; waste water; Bacteria; Biodegradation, Environmental; Metagenome; Metagenomics; Phylogeny; Sewage; Waste Water
Año:2016
Volumen:82
Número:17
Página de inicio:5186
Página de fin:5196
DOI: http://dx.doi.org/10.1128/AEM.00916-16
Título revista:Applied and Environmental Microbiology
Título revista abreviado:Appl. Environ. Microbiol.
ISSN:00992240
CODEN:AEMID
CAS:Sewage; Waste Water
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v82_n17_p5186_Ibarbalz

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Citas:

---------- APA ----------
Ibarbalz, F.M., Orellana, E., Figuerola, E.L.M. & Erijman, L. (2016) . Shotgun metagenomic profiles have a high capacity to discriminate samples of activated sludge according to wastewater type. Applied and Environmental Microbiology, 82(17), 5186-5196.
http://dx.doi.org/10.1128/AEM.00916-16
---------- CHICAGO ----------
Ibarbalz, F.M., Orellana, E., Figuerola, E.L.M., Erijman, L. "Shotgun metagenomic profiles have a high capacity to discriminate samples of activated sludge according to wastewater type" . Applied and Environmental Microbiology 82, no. 17 (2016) : 5186-5196.
http://dx.doi.org/10.1128/AEM.00916-16
---------- MLA ----------
Ibarbalz, F.M., Orellana, E., Figuerola, E.L.M., Erijman, L. "Shotgun metagenomic profiles have a high capacity to discriminate samples of activated sludge according to wastewater type" . Applied and Environmental Microbiology, vol. 82, no. 17, 2016, pp. 5186-5196.
http://dx.doi.org/10.1128/AEM.00916-16
---------- VANCOUVER ----------
Ibarbalz, F.M., Orellana, E., Figuerola, E.L.M., Erijman, L. Shotgun metagenomic profiles have a high capacity to discriminate samples of activated sludge according to wastewater type. Appl. Environ. Microbiol. 2016;82(17):5186-5196.
http://dx.doi.org/10.1128/AEM.00916-16