Artículo

Kleiman, F.; Pietrokovsky, S.; Gil, S.; Wisnivesky-Colli, C. "Comparison of two coprological methods for the veterinary diagnosis of fasciolosis" (2005) Arquivo Brasileiro de Medicina Veterinaria e Zootecnia. 57(2):181-185
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Abstract:

The sensitivity and utility of a standard faecal sedimentation method (FSM) and a modified stool sieving staining method (FSSM), both currently employed for the diagnosis of Fasciola hepatica infection were compared. Faecal samples were obtained from 51 bovines of an endemic area for fasciolosis in Southwestern Argentina. Each sample was placed in a recipient containing 5% formalin. Eight millilitres of the suspension, equivalent to 2g of faeces, were used for each of the two methods tested. The number of eggs found per sample was recorded. The proportion of positive samples obtained by the FSSM (27/51) was significantly higher than that by the FSM (11/51) (P<0.05). The percent of agreement between methods was 41%. Over a total of 27 positive samples detected by the FSSM, the FSM missed 16, yielding 60% false negative samples. The FSSM enhanced 2.5 times the sensitivity of diagnosis. The complexity of the FSM may decrease its sensitivity through missing and loss of eggs during sample processing. These results confirmed that the commonly used FSM underestimates the prevalence and the egg output in cattle and that the FSSM is a more reliable diagnostic method.

Registro:

Documento: Artículo
Título:Comparison of two coprological methods for the veterinary diagnosis of fasciolosis
Autor:Kleiman, F.; Pietrokovsky, S.; Gil, S.; Wisnivesky-Colli, C.
Filiación:Departamento de Ciencias Biológicas - 4o Piso, Pabellón II, Ciudad Universitaria, Av. Cantilo s/n, C1428EHA Buenos Aires, Argentina
Palabras clave:Cattle; Coprological method; Diagnosis; Fasciola hepatica; Bos taurus; Fasciola hepatica
Año:2005
Volumen:57
Número:2
Página de inicio:181
Página de fin:185
DOI: http://dx.doi.org/10.1590/S0102-09352005000200007
Título revista:Arquivo Brasileiro de Medicina Veterinaria e Zootecnia
Título revista abreviado:Arq. Bras. Med. Vet. Zootec.
ISSN:01020935
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01020935_v57_n2_p181_Kleiman

Referencias:

  • Abidu, M., Scherer, P.O., Silva Carneiro, V., Estudo comparativo entre técnicas coproparasitológicas para diagnóstico de Fasciola hepatica em bovinos (1996) Rev. Bras. Ciênc. Vet., 3, pp. 1-3
  • Anderson, N., Luong, T.T., Vo, N.G., The sensitivity and specificity of two methods for detecting Fasciola infections in cattle (1999) Vet. Parasitol., 83, pp. 15-24
  • Boray, J.C., Flukes of domestic animals (1985) Parasites, Pests and Predators, pp. 179-218. , GAAFAR, S.M.; HOWARD W.E.; MARSH R.E. (Eds.). World Animal Science, Elsevier
  • Boray, J.C., Pearson, I.G., The anthelmintic efficiency of tetrachlorodifluoroethane in sheep infested with Fasciola hepatica (1960) Aust. Vet. J., 36, pp. 331-336
  • Dennis, W.R., Stone, W.M., Swanson, L.E., A new laboratory and field diagnostic test for fluke ova in feces (1954) J. Am. Vet. Med. Assoc., 124, pp. 47-50
  • Dixon, R.C., Wescott, R.B., A fast and accurate fecal examination technique for diagnosis of Fasciola hepatica (1987) Annual Meeting of the American Association of Veterinary Parasitologists, 3, p. 28. , Portland. Proceedings... Portland: AAVP. (Abstract)
  • Duménigo, B.E., Espino, A.M., Finlay, C.M., Kinetics of antibody-based antigen detection in serum and faeces of sheep experimentally infected with Fasciola hepatica (2000) Vet. Parasitol., 89, pp. 153-161
  • Fleiss, J.L., (1981) Statistical Methods for Rates and Proportions. 2.ed., 255p. , New York: John Wiley & Sons
  • Girão, E.S., Ueno, H., Técnica quatro tamises metálicos (1994) Manual Para Diagnóstico das Helmintoses de Ruminantes. 3.ed., p. 62. , UENO, H.; GONÇALVES, P.C. Japan International Cooperation Agency and Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
  • Gordis, L., Assessing the validity and reliability of diagnostic and screening tests (2000) Epidemiology. 2.ed., pp. 63-81. , Pennsylvania: W.B. Saunders
  • Grock, R., Morales, G., Vaca, J.L., Fascioliasis in sheep in the human high endemic region of the northern Bolivian Altiplano (1998) Res. Rev. Parasitol., 58, pp. 95-101
  • Hope Cawdery, M.J., Review of the economic importance of fascioliasis in sheep and cattle (1984) Ir. Vet. News, 7, pp. 14-22
  • Malone, J.B., The landscape epidemiology of fasciolosis: Geographic determinants of disease risk (1994) Immunology, Pathobiology and Control of Fasciolosis, 8, pp. 65-81. , BORAY, J.C. (Ed.). CONFERENCE ICOPA, Izmir, Turkey
  • (1994) Medios Auxiliares Para El Diagnóstico de Las Parasitosis Intestinales, pp. 1-6. , Francia: Servicios Gráficos de la OMS
  • Parr, S.L., Gray, J.S., A strategic dosing scheme for the control of fasciolosis in cattle and sheep in Ireland (2000) Vet. Parasitol., 88, pp. 187-197
  • Ritchie, L.S., An ether sedimentation technique for routine stool examinations (1948) Bull. U.S. Army Med. Depart., 8, p. 326
  • Shore García, L., Ash, L.R., (1973) Diagnóstico Parasitológico, 157p. , Buenos Aires: Panamericana
  • Ward, M.P., Lyndal-Murphy, M., Baldock, F.C., Evaluation of a composite method for counting helminth eggs in cattle faeces (1997) Vet. Parasitol., 73, pp. 181-187
  • Zukowski, S.H., Wayne Wilkerson, G., Malone, J.B., Fasciolosis in cattle in Louisiana. II. Development of a system to use soil maps in a geographic information system to estimate disease risk on Louisiana coastal marsh rangeland (1993) Vet. Parasitol., 47, pp. 51-65

Citas:

---------- APA ----------
Kleiman, F., Pietrokovsky, S., Gil, S. & Wisnivesky-Colli, C. (2005) . Comparison of two coprological methods for the veterinary diagnosis of fasciolosis. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 57(2), 181-185.
http://dx.doi.org/10.1590/S0102-09352005000200007
---------- CHICAGO ----------
Kleiman, F., Pietrokovsky, S., Gil, S., Wisnivesky-Colli, C. "Comparison of two coprological methods for the veterinary diagnosis of fasciolosis" . Arquivo Brasileiro de Medicina Veterinaria e Zootecnia 57, no. 2 (2005) : 181-185.
http://dx.doi.org/10.1590/S0102-09352005000200007
---------- MLA ----------
Kleiman, F., Pietrokovsky, S., Gil, S., Wisnivesky-Colli, C. "Comparison of two coprological methods for the veterinary diagnosis of fasciolosis" . Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, vol. 57, no. 2, 2005, pp. 181-185.
http://dx.doi.org/10.1590/S0102-09352005000200007
---------- VANCOUVER ----------
Kleiman, F., Pietrokovsky, S., Gil, S., Wisnivesky-Colli, C. Comparison of two coprological methods for the veterinary diagnosis of fasciolosis. Arq. Bras. Med. Vet. Zootec. 2005;57(2):181-185.
http://dx.doi.org/10.1590/S0102-09352005000200007