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Two polysaccharide fractions isolated from Hypnea musciformis after room temperature- and hot water extraction, soluble after KCl precipitation of the more abundant carrageenans, were subfractionated by ion-exchange chromatography eluting with increasing concentrations of NaCl. The lowest NaCl concentration (0.2 M) eluted agarans. The DL-hybrids (or mixtures) eluted at intermediate concentrations of NaCl. The D/L-galactose ratio and the sulfate proportion increased with the NaCl concentration. Different types of substitution were present, mainly at C-3 with sulfate, Xyl and methylated Gal stubs, as well as low amounts of 3,6-AnGal. A novel constituent, identified as 3-C-carboxy-D-erythrose1 in its β-furanosic form, was found linked to C-6 of β-Gal units. A search carried out in other species like Iridaea undulosa and Kappaphycus alvarezii also revealed the same constituent. Finally, the late-eluting fractions were mostly carrageenans, with a structure consistent with that of a κ/ι/ν-carrageenan hybrid. © 2016 Elsevier Ltd


Documento: Artículo
Título:Minor polysaccharidic constituents from the red seaweed Hypnea musciformis. Appearance of a novel branched uronic acid
Autor:Cosenza, V.A.; Navarro, D.A.; Stortz, C.A.
Filiación:Departamento de Química Orgánica-CIHIDECAR/CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, 1428, Argentina
Palabras clave:3-C-carboxy-D-erythrofuranose; Agarans; Apiuronic acid; Carrageenans; Hypnea musciformis; Chromatographic analysis; Ion chromatography; Ion exchange; 3-C-carboxy-D-erythrofuranose; Agarans; Carrageenans; Hot water extraction; Hypnea musciformis; Ion exchange chromatography; Kappaphycus alvarezii; NaCl concentration; Chromatography; carrageenan; polysaccharide; uronic acid; chemistry; isolation and purification; red alga; seaweed; Carrageenan; Polysaccharides; Rhodophyta; Seaweed; Uronic Acids
Página de inicio:156
Página de fin:166
Título revista:Carbohydrate Polymers
Título revista abreviado:Carbohydr Polym
CAS:carrageenan, 9000-07-1, 9049-05-2, 9061-82-9, 9064-57-7; Carrageenan; Polysaccharides; Uronic Acids


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---------- APA ----------
Cosenza, V.A., Navarro, D.A. & Stortz, C.A. (2017) . Minor polysaccharidic constituents from the red seaweed Hypnea musciformis. Appearance of a novel branched uronic acid. Carbohydrate Polymers, 157, 156-166.
---------- CHICAGO ----------
Cosenza, V.A., Navarro, D.A., Stortz, C.A. "Minor polysaccharidic constituents from the red seaweed Hypnea musciformis. Appearance of a novel branched uronic acid" . Carbohydrate Polymers 157 (2017) : 156-166.
---------- MLA ----------
Cosenza, V.A., Navarro, D.A., Stortz, C.A. "Minor polysaccharidic constituents from the red seaweed Hypnea musciformis. Appearance of a novel branched uronic acid" . Carbohydrate Polymers, vol. 157, 2017, pp. 156-166.
---------- VANCOUVER ----------
Cosenza, V.A., Navarro, D.A., Stortz, C.A. Minor polysaccharidic constituents from the red seaweed Hypnea musciformis. Appearance of a novel branched uronic acid. Carbohydr Polym. 2017;157:156-166.