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Abstract:

Sulfated polysaccharides were localized in the cuticle, cortex and medulla of the gametophyte thallus, being more concentrated in the intercellular matrix than in the cell walls. During the water extraction sequence, a small percentage of galactan sulfates (5.1% of dry seaweed) with average low Mr (6-11.4 kDa) were extracted at room temperature without disturbing the cellular arrangement, while sulfated galactans of average medium Mr (18-45 kDa) were obtained by further hot-water extractions (52.4% of dry seaweed), with diorganization of the tissue. The residue (40.0% of dry seaweed) still contained carrageenan-type (major) and agaran-type (minor) galactans. Part of these galactans was extracted with 8.4% LiCl solution in DMSO, from which "pure" κ/ι-carrageenans were isolated. Carrageenans and agarans were extracted in a ratio 1:0.5, showing the highest amount of agaran-structures for a carrageenophyte. The galactans comprise alternating 4-sulfated (major) and non-sulfated (minor) 3-linked β-d-galactopyranose units, and 4-linked α-galactopyranose units with the following substitutions: (i) non-sulfated and 2-sulfated 3,6-anhydro-α-d-galactopyranose residues in the carrageenan-structures, which belong to the κ-family (κ/ι-carrageenans); (ii) 3-sulfated α-l-galactopyranose units and 2-sulfated 3,6-anhydro-α-l-galactopyranose residues in the agaran-structures. Alkaline treatment and alkaline dialysis of the main extracts gave "pure" κ/ι-carrageenans, showing that carrageenan molecules are extracted together with low Mr agarans or agaran-dl-hybrids. © 2008 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:The system of sulfated galactans from the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta): Location and structural analysis
Autor:Estevez, J.M.; Ciancia, M.; Cerezo, A.S.
Filiación:Departamento de Química Orgánica (CIHIDECAR-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria - Pabellon 2, 1428 Buenos Aires, Argentina
Cátedra de Química Orgánica, Departamento de Biología Aplicada y Alimentos (CIHIDECAR-CONICET), Facultad de Agronomía, Avda. San Martín 4453, 1417 Buenos Aires, Argentina
Palabras clave:Agarans; Carrageenans; Cell wall; Gymnogongrus torulosus (Phyllophoraceae); l-Galactose; Red seaweed; Sulfated galactans; Algae; Cell membranes; Dialysis; Extraction; Molecular structure; Tissue; Agarans; Carrageenans; Gymnogongrus torulosus (Phyllophoraceae); l-Galactose; Red seaweed; Sulfated galactans; Polysaccharides; Gymnogongrus torulosus; Phyllophoraceae; Rhodophyta
Año:2008
Volumen:73
Número:4
Página de inicio:594
Página de fin:605
DOI: http://dx.doi.org/10.1016/j.carbpol.2007.12.033
Título revista:Carbohydrate Polymers
Título revista abreviado:Carbohydr Polym
ISSN:01448617
CODEN:CAPOD
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01448617_v73_n4_p594_Estevez

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Citas:

---------- APA ----------
Estevez, J.M., Ciancia, M. & Cerezo, A.S. (2008) . The system of sulfated galactans from the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta): Location and structural analysis. Carbohydrate Polymers, 73(4), 594-605.
http://dx.doi.org/10.1016/j.carbpol.2007.12.033
---------- CHICAGO ----------
Estevez, J.M., Ciancia, M., Cerezo, A.S. "The system of sulfated galactans from the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta): Location and structural analysis" . Carbohydrate Polymers 73, no. 4 (2008) : 594-605.
http://dx.doi.org/10.1016/j.carbpol.2007.12.033
---------- MLA ----------
Estevez, J.M., Ciancia, M., Cerezo, A.S. "The system of sulfated galactans from the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta): Location and structural analysis" . Carbohydrate Polymers, vol. 73, no. 4, 2008, pp. 594-605.
http://dx.doi.org/10.1016/j.carbpol.2007.12.033
---------- VANCOUVER ----------
Estevez, J.M., Ciancia, M., Cerezo, A.S. The system of sulfated galactans from the red seaweed Gymnogongrus torulosus (Phyllophoraceae, Rhodophyta): Location and structural analysis. Carbohydr Polym. 2008;73(4):594-605.
http://dx.doi.org/10.1016/j.carbpol.2007.12.033