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Abstract:

The aim is to describe the molecular epidemiology and perform a genomic characterization of hepatitis B virus (HBV) circulating in Mar del Plata and to identify the origin and diversification patterns of the most prevalent genotype. The S gene and the region encompassing the X gene, basal core promoter (BCP), and precore (preC) was analyzed in 56 samples. They were genotyped as: 80% F1b, 9% A2, 7% D3, and 2% D1. A recombinant F4/D2 genome was detected. The double substitution G1764A/A1762T at the BCP (reduced HBeAg expression) was found in 20% F1b, 2% A2, 2% D1, and 2% D3 samples. A unique D3 presented the G1896A substitution at the preC (HBeAg negative phenotype). A 13% of the samples showed mutations at the HBsAg “a” immunodeterminant (escape from neutralizing antibodies). Mutations at the polymerase (antiviral resistance) were found in 52% of the samples. Coalescent analysis of subgenotype F1b, the most prevalent in the city, showed that viral diversification in Mar del Plata started by year 2000. F1b was the most prevalent genotype detected, being a characteristic of actual HBV infections in Mar del Plata. Local HBV exhibit clinically relevant mutations, but a minority of them was shown to be associated to potential vaccination escape or antiviral resistance. Nevertheless, further studies are needed to determine whether any of these mutants could pose a threat to prevention, diagnosis, or treatment. © 2019 Wiley Periodicals, Inc.

Registro:

Documento: Artículo
Título:Hepatitis B virus in Mar del Plata, Argentina: Genomic characterization and evolutionary analysis of subgenotype F1b
Autor:Torres, M.C.; Civetta, E.; D’amico, C.; Barbini, L.
Filiación:Departamento de Química, FCEyN, UNMdP, Buenos Aires, Argentina
Unidad de Hepatología y Alcoholismo, HIGA Dr. O. Alende, Mar del Plata, Argentina
Centro de Especialidades Médicas Ambulatorias, Unidad de Hepatología, Mar del Plata, Argentina
Palabras clave:Argentina; genomic characterization; genotypes; hepatitis B virus; molecular epidemiology
Año:2019
Volumen:91
Número:5
Página de inicio:791
Página de fin:802
DOI: http://dx.doi.org/10.1002/jmv.25383
Título revista:Journal of Medical Virology
Título revista abreviado:J. Med. Virol.
ISSN:01466615
CODEN:JMVID
Registro:http://digital.bl.fcen.uba.ar/collection/paper/document/paper_01466615_v91_n5_p791_Torres

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Citas:

---------- APA ----------
Torres, M.C., Civetta, E., D’amico, C. & Barbini, L. (2019) . Hepatitis B virus in Mar del Plata, Argentina: Genomic characterization and evolutionary analysis of subgenotype F1b. Journal of Medical Virology, 91(5), 791-802.
http://dx.doi.org/10.1002/jmv.25383
---------- CHICAGO ----------
Torres, M.C., Civetta, E., D’amico, C., Barbini, L. "Hepatitis B virus in Mar del Plata, Argentina: Genomic characterization and evolutionary analysis of subgenotype F1b" . Journal of Medical Virology 91, no. 5 (2019) : 791-802.
http://dx.doi.org/10.1002/jmv.25383
---------- MLA ----------
Torres, M.C., Civetta, E., D’amico, C., Barbini, L. "Hepatitis B virus in Mar del Plata, Argentina: Genomic characterization and evolutionary analysis of subgenotype F1b" . Journal of Medical Virology, vol. 91, no. 5, 2019, pp. 791-802.
http://dx.doi.org/10.1002/jmv.25383
---------- VANCOUVER ----------
Torres, M.C., Civetta, E., D’amico, C., Barbini, L. Hepatitis B virus in Mar del Plata, Argentina: Genomic characterization and evolutionary analysis of subgenotype F1b. J. Med. Virol. 2019;91(5):791-802.
http://dx.doi.org/10.1002/jmv.25383