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Abstract:

Partially purified extracts from leaves of Melia azedarach L. (MA) exert a broad range of antiviral effects on DNA and RNA viruses. The effect of MA on different stages of Sindbis virus replicative cycle in BHK cells was investigated. Under one-step growth conditions MA afforded a greater than 90% inhibition in virus yield if added to the cell cultures 2 h before or after infection, and when added 4 h after infection MA still caused a greater than 80% inhibition. Analysis of early events following Sindbis virus infection showed that MA did not affect viral adsorption to or penetration in BHK cell. In contrast, viral RNA and protein synthesis was almost totally inhibited in cells pretreated with MA 2 h before infection, while cellular macromolecular synthesis was similar in MA-treated and untreated cell cultures. © 1987.

Registro:

Documento: Artículo
Título:Antiviral effects of Melia azedarach L. leaves extracts on Sindbis virus-infected cells
Autor:Wachsman, M.B.; Damonte, E.B.; Coto, C.E.; De Torres, R.A.
Filiación:Laboratorio de Virologia, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina, Argentina
Departamento de Microbiologia e Inmunologia, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Melia azedarach L; Sindbis virus; antivirus agent; plant extract; cell culture; drug analysis; drug mechanism; electrophoresis; higher plant; in vitro study; melia azedarach; nonhuman; protein synthesis; rna synthesis; sindbis virus; therapy; virus cell interaction; virus infection; Antiviral Agents; Cell Adhesion; Cell Line; Plant Extracts; RNA, Viral; Sindbis Virus; Support, Non-U.S. Gov't; Viral Proteins; Virus Replication
Año:1987
Volumen:8
Número:1
Página de inicio:1
Página de fin:12
DOI: http://dx.doi.org/10.1016/0166-3542(87)90083-0
Título revista:Antiviral Research
Título revista abreviado:Antiviral Res.
ISSN:01663542
CODEN:ARSRD
CAS:Antiviral Agents; Plant Extracts; RNA, Viral; Viral Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v8_n1_p1_Wachsman

Referencias:

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Citas:

---------- APA ----------
Wachsman, M.B., Damonte, E.B., Coto, C.E. & De Torres, R.A. (1987) . Antiviral effects of Melia azedarach L. leaves extracts on Sindbis virus-infected cells. Antiviral Research, 8(1), 1-12.
http://dx.doi.org/10.1016/0166-3542(87)90083-0
---------- CHICAGO ----------
Wachsman, M.B., Damonte, E.B., Coto, C.E., De Torres, R.A. "Antiviral effects of Melia azedarach L. leaves extracts on Sindbis virus-infected cells" . Antiviral Research 8, no. 1 (1987) : 1-12.
http://dx.doi.org/10.1016/0166-3542(87)90083-0
---------- MLA ----------
Wachsman, M.B., Damonte, E.B., Coto, C.E., De Torres, R.A. "Antiviral effects of Melia azedarach L. leaves extracts on Sindbis virus-infected cells" . Antiviral Research, vol. 8, no. 1, 1987, pp. 1-12.
http://dx.doi.org/10.1016/0166-3542(87)90083-0
---------- VANCOUVER ----------
Wachsman, M.B., Damonte, E.B., Coto, C.E., De Torres, R.A. Antiviral effects of Melia azedarach L. leaves extracts on Sindbis virus-infected cells. Antiviral Res. 1987;8(1):1-12.
http://dx.doi.org/10.1016/0166-3542(87)90083-0