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Abstract:

A recent addition to the arsenal of tools for glycome analysis is the use of metabolic labels that allow covalent tagging of glycans with imaging probes. In this work we show that N-azidoglucosamine was successfully incorporated into glycolipidic structures of Plasmodium falciparum intraerythrocytic stages. The ability to tag glycoconjugates selectively with a fluorescent reporter group permits TLC detection of the glycolipids providing a new method to quantify dynamic changes in the glycosylation pattern and facilitating direct mass spectrometry analyses. Presence of glycosylphosphatidylinositol and glycosphingolipid structures was determined in the different extracts. Furthermore, the fluorescent tag was used as internal matrix for the MALDI experiment making even easier the analysis. © 2012 Elsevier B.V.

Registro:

Documento: Artículo
Título:Metabolic oligosaccharide engineering of Plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry
Autor:Piñero, T.; Peres, V.J.; Katzin, A.; Couto, A.S.
Filiación:CIHIDECAR, Departamento de Química Orgánica, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina
Departamento de Parasitología, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil
Palabras clave:Glycolipids; Metabolic oligosaccharide engineering; P. falciparum; glucosamine derivative; glycolipid; glycosphingolipid; glycosylphosphatidylinositol; n azidoglucosamine; oligosaccharide; unclassified drug; article; erythrocyte; fluorescence analysis; glycosylation; mass spectrometry; matrix assisted laser desorption ionization time of flight mass spectrometry; Plasmodium falciparum; priority journal; structure analysis; thin layer chromatography; ultraviolet radiation; Chromatography, Thin Layer; Erythrocytes; Fluorescent Dyes; Glycolipids; Glycosphingolipids; Glycosylation; Glycosylphosphatidylinositols; Mass Spectrometry; Metabolic Engineering; Oligosaccharides; Plasmodium falciparum; Staining and Labeling; Plasmodium falciparum
Año:2012
Volumen:182
Número:1-2
Página de inicio:88
Página de fin:92
DOI: http://dx.doi.org/10.1016/j.molbiopara.2011.12.008
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:Fluorescent Dyes; Glycolipids; Glycosphingolipids; Glycosylphosphatidylinositols; Oligosaccharides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v182_n1-2_p88_Pinero

Referencias:

  • Wellems, T.E., Hayton, K., Faihurst, R.M., The impact of malaria parasitism: From corpuscles to communities (2009) J Clin Invest, 119, pp. 2496-2505
  • Mitamura, T., Palacpac, N.M.Q., Lipid metabolism in Plasmodium falciparum-infected erythrocytes: Possible new targets for malaria chemotherapy (2003) Microbes and Infection, 5 (6), pp. 545-552. , DOI 10.1016/S1286-4579(03)00070-4
  • Gerold, P., Dieckmann-Schuppert, A., Schwarz, R.T., Glycosylphosphatidylinositols synthesized by asexual erythrocytic stages of the malarial parasite Plasmodium falciparum. Candidates for plasmodial glycosylphosphatidylinositol membrane anchor precursors and pathogenicity factors (1994) J Biol Chem, 269, pp. 2597-2606
  • Gowda, D.C., Gupta, P., Davidson, E.A., Glycosylphosphatidylinositol anchors represent the major carbohydrate modification in proteins of intraerythrocytic stage Plasmodium falciparum (1997) Journal of Biological Chemistry, 272 (10), pp. 6428-6439. , DOI 10.1074/jbc.272.10.6428
  • Gerold, P., Schofield, L., Blackman, M.J., Holder, A.A., Schwarz, R.T., Structural analysis of the glycosyl-phosphatidylinositol membrane anchor of the merozoite surface proteins-1 and -2 of Plasmodium falciparum (1996) Molecular and Biochemical Parasitology, 75 (2), pp. 131-143. , DOI 10.1016/0166-6851(95)02518-9
  • Schmidt, A., Schwarz, R.T., Gerold, P., Plasmodium falciparum: Asexual erythrocytic stages synthesize two structurally distinct free and protein-bound glycosylphosphatidylinositols in a maturation-dependent manner (1998) Experimental Parasitology, 88 (2), pp. 95-102. , DOI 10.1006/expr.1998.4241
  • Berhe, S., Schofield, L., Schwarz, R.T., Gerold, P., Conservation of structure among glycosylphosphatidylinositol toxins from different geographic isolates of Plasmodium falciparum (1999) Molecular and Biochemical Parasitology, 103 (2), pp. 273-278. , DOI 10.1016/S0166-6851(99)00125-5, PII S0166685199001255
  • Naik, R.S., Branch, O.H., Woods, A.S., Vijaykumar, M., Perkins, D.J., Nahlen, B.L., Glycosylphosphatidylinositol anchors of Plasmodium falciparum: Molecular characterization and naturally elicited antibody response that may provide immunity to malaria pathogenesis (2000) J Exp Med, 192, pp. 1563-1576
  • Haldar, K., Uyetake, L., Ghori, N., Elmendorf, H.G., Li, W.L., The accumulation and metabolism of a fluorescent ceramide derivative in Plasmodium falciparum-infected erythrocytes (1991) Mol Biochem Parasitol, 49, pp. 143-156
  • Ansorge, I., Jeckel, D., Wieland, F., Lingelbach, K., Plasmodium falciparum-infected erythrocytes utilize a synthetic truncated ceramide precursor for synthesis and secretion of truncated sphingomyelin (1995) Biochem J, 308, pp. 335-341
  • Gerold, P., Schwarz, R.T., Biosynthesis of glycosphingolipids de-novo by the human malaria parasite Plasmodium falciparum (2000) Mol Biochem Parasitol, 112, pp. 29-37
  • Marechal, E., Azzouz, N., Santos De MacEdo, C., Block, M.A., Feagin, J.E., Schwarz, R.T., (2002) Synthesis of Chloroplast Galactolipids in Apicomplexan Parasites Eukaryotic Cell, 1, pp. 653-656
  • Couto, A.S., Caffaro, C., Uhrig, M.L., Kimura, E., Peres, V.J., Merino, E.F., Katzin, A.M., Erra-Balsells, R., Glycosphingolipids in Plasmodium falciparum: Presence of an active glucosylceramide synthase (2004) European Journal of Biochemistry, 271 (11), pp. 2204-2214. , DOI 10.1111/j.1432-1033.2004.04150.x
  • Landoni, M., Duschak, V.G., Peres, V.J., Nonami, H., Erra-Balsells, R., Katzin, A.M., Couto, A.S., Plasmodium falciparum biosynthesizes sulfoglycosphingolipids (2007) Molecular and Biochemical Parasitology, 154 (1), pp. 22-29. , DOI 10.1016/j.molbiopara.2007.03.014, PII S0166685107001041
  • Laughlin, S.T., Agard, N.J., Baskin, J.M., Carrico, I.S., Chang, P.V., Ganguli, A.S., Hangauer, M.J., Bertozzi, C.R., Metabolic Labeling of Glycans with Azido Sugars for Visualization and Glycoproteomics (2006) Methods in Enzymology, 415, pp. 230-250. , DOI 10.1016/S0076-6879(06)15015-6, PII S0076687906150156, Glycobiology
  • Gerold, P., Jung, N., Azzouz, N., Freiberg, N., Kobe, S., Schwarz, R.T., Biosynthesis of glycosylphosphatidylinositols of Plasmodium falciparum in a cell-free incubation system: Inositol acylation is needed for mannosylation of glycosylphosphatidylinositols (1999) Biochemical Journal, 344 (3), pp. 731-738. , DOI 10.1042/0264-6021:3440731
  • Debierre-Grockiego, F., Schwarz, R.T., Immunological reactions in response to apicomplexan glycosylphosphatidylinositols (2010) Glycobiology, 20, pp. 801-811
  • Delorenzi, M., Sexton, A., Shams-Eldin, H., Schwarz, R.T., Speed, T., Schofield, L., Genes for glycosylphosphatidylinositol toxin biosynthesis in Plasmodium falciparum (2002) Infection and Immunity, 70 (8), pp. 4510-4522. , DOI 10.1128/IAI.70.8.4510-4522.2002
  • Landoni, M., Duschak, V.G., Erra-Balsells, R., Couto, A.S., UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrix (2008) J Am Soc Mass Spectrom, 19, pp. 923-926
  • Kimura, E.A., Couto, A.S., Peres, V.J., Casal, O.L., Katzin, A.M., N-linked glycoproteins are related to schizogony of the intraerythrocytic stage in Plasmodium falciparum (1996) Journal of Biological Chemistry, 271 (24), pp. 14452-14461. , DOI 10.1074/jbc.271.24.14452

Citas:

---------- APA ----------
Piñero, T., Peres, V.J., Katzin, A. & Couto, A.S. (2012) . Metabolic oligosaccharide engineering of Plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry. Molecular and Biochemical Parasitology, 182(1-2), 88-92.
http://dx.doi.org/10.1016/j.molbiopara.2011.12.008
---------- CHICAGO ----------
Piñero, T., Peres, V.J., Katzin, A., Couto, A.S. "Metabolic oligosaccharide engineering of Plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry" . Molecular and Biochemical Parasitology 182, no. 1-2 (2012) : 88-92.
http://dx.doi.org/10.1016/j.molbiopara.2011.12.008
---------- MLA ----------
Piñero, T., Peres, V.J., Katzin, A., Couto, A.S. "Metabolic oligosaccharide engineering of Plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry" . Molecular and Biochemical Parasitology, vol. 182, no. 1-2, 2012, pp. 88-92.
http://dx.doi.org/10.1016/j.molbiopara.2011.12.008
---------- VANCOUVER ----------
Piñero, T., Peres, V.J., Katzin, A., Couto, A.S. Metabolic oligosaccharide engineering of Plasmodium falciparum intraerythrocytic stages allows direct glycolipid analysis by mass spectrometry. Mol. Biochem. Parasitol. 2012;182(1-2):88-92.
http://dx.doi.org/10.1016/j.molbiopara.2011.12.008