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Abstract:

The major cysteine proteinase (cruzipain) purified from Trypanosoma cruzi epimastigotes catalyzes its own degradation in the presence of β-mercaptoethanol, at 56°C and pH 6. The reaction is affected by the same inhibitors which inhibit the azocaseinase activity, and yields a major 25-kDa fragment, which contains carbohydrate, few, if any, aromatic amino acids, and presents a proline-rich N-terminus (GPGPXPEP...), in addition to a number of small peptides, which can be isolated by reversed-phase HPLC, but are lost during electrophoresis. The results, together with recently published evidence of Mottram et al. and Eakin et al., are compatible with a structure for cruzipain consisting of a conventional cysteine proteinase moiety, linked to a long C-terminal extension including the 25-kDa fragment, which would contain a high proportion of the carbohydrate and the proline residues present in the original 60-kDa molecule. © 1991.

Registro:

Documento: Artículo
Título:Self-proteolysis of the cysteine proteinase, cruzipain, from Trypanosoma cruzi gives a major fragment corresponding to its carboxy-terminal domain
Autor:Hellman, U.; Wernstedt, C.; Cazzulo, J.J.
Filiación:Ludwig Institute for Cancer Research, Uppsala Branch, Uppsala, Sweden
Instituto de Investigaciones Bioquímicas 'Luis F. Leloir', Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Palabras clave:Cruzipain; Cysteine proteinase; Self-proteolysis; Trypanosoma cruzi; cysteine proteinase; animal cell; article; epimastigote; nonhuman; priority journal; trypanosoma cruzi; Amino Acid Sequence; Animal; Carbohydrates; Chromatography, High Pressure Liquid; Cysteine Endopeptidases; Electrophoresis, Polyacrylamide Gel; Mercaptoethanol; Molecular Sequence Data; Peptide Fragments; Sequence Homology, Nucleic Acid; Support, Non-U.S. Gov't; Trypanosoma brucei brucei; Trypanosoma cruzi; Trypsin; Animalia; Trypanosoma; Trypanosoma cruzi
Año:1991
Volumen:44
Número:1
Página de inicio:15
Página de fin:21
DOI: http://dx.doi.org/10.1016/0166-6851(91)90216-S
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:Carbohydrates; cruzipain, EC 3.4.22.-; Cysteine Endopeptidases, EC 3.4.22; Mercaptoethanol, 60-24-2; Peptide Fragments; Trypsin, EC 3.4.21.4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v44_n1_p15_Hellman

Referencias:

  • Bontempi, Franke de Cazzulo, Ruiz, Cazzulo, Purification and some properties of an acidic protease from epimastigotes of Trypanosoma cruzi (1984) Comp. Biochem. Physiol., 77 B, pp. 599-604
  • Campetella, Martinez, Cazzulo, A major cysteine proteinase is developmentally regulated in Trypanosoma cruzi (1990) FEMS Microbiol. Lett., 67, pp. 145-150
  • Cazzulo, Cazzulo Franke, Martinez, Franke de Cazzulo, Some kinetic properties of a cysteine proteinase (Cruzipain) from Trypanosoma cruzi (1990) Biochim. Biophys. Acta, 1037, pp. 186-191
  • Cazzulo, Couso, Raimondi, Wernstedt, Hellman, Further characterization and partial amino acid sequence of a cysteine proteinase from Trypanosoma cruzi (1989) Mol. Biochem. Parasitol., 33, pp. 33-41
  • Bontempi, Martínez, Cazzulo, Subcellular localization of a cysteine proteinase from Trypanosoma cruzi (1989) Mol. Biochem. Parasitol., 33, pp. 43-47
  • Cazzulo, Hellman, Couso, Parodi, Amino acid and carbohydrate composition of a lysosomal cysteine proteinase from Trypanosoma cruzi. Absence of phosphorylated mannose residues (1990) Mol. Biochem. Parasitol., 38, pp. 41-48
  • Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685
  • Herzberg, Grigorescu, Edge, Spiro, Kahn, characterization of insulin receptor carbohydrate by comparison of chemical and enzymatic deglycosylation (1985) Biochem. Biophys. Res. Commun., 129, pp. 789-796
  • Mottram, North, Barry, Coombs, A cysteine proteinase cDNA from Trypanosoma brucei predicts an enzyme with an unusual C-terminal extension (1989) FEBS Lett., 258, pp. 211-215
  • Eakin, Bouvier, Sakanari, Craik, McKerrow, Amplification and sequencing of genomic DNA fragments encoding cysteine proteinase from protozoan parasites (1990) Mol. Biochem. Parasitol., 39, pp. 1-8
  • Glazer, Smith, Papain and other plant su;fhydryl proteolytic enzymes (1971) The Enzymes, 3, pp. 501-546. , 3rd Ed., P.D. Boyer, Academic Press, New York and London
  • Barrett, Kirschke, Cathepsin B, Cathepsin H, and Cathepsin L (1981) Methods Enzymol., 80, pp. 535-561

Citas:

---------- APA ----------
Hellman, U., Wernstedt, C. & Cazzulo, J.J. (1991) . Self-proteolysis of the cysteine proteinase, cruzipain, from Trypanosoma cruzi gives a major fragment corresponding to its carboxy-terminal domain. Molecular and Biochemical Parasitology, 44(1), 15-21.
http://dx.doi.org/10.1016/0166-6851(91)90216-S
---------- CHICAGO ----------
Hellman, U., Wernstedt, C., Cazzulo, J.J. "Self-proteolysis of the cysteine proteinase, cruzipain, from Trypanosoma cruzi gives a major fragment corresponding to its carboxy-terminal domain" . Molecular and Biochemical Parasitology 44, no. 1 (1991) : 15-21.
http://dx.doi.org/10.1016/0166-6851(91)90216-S
---------- MLA ----------
Hellman, U., Wernstedt, C., Cazzulo, J.J. "Self-proteolysis of the cysteine proteinase, cruzipain, from Trypanosoma cruzi gives a major fragment corresponding to its carboxy-terminal domain" . Molecular and Biochemical Parasitology, vol. 44, no. 1, 1991, pp. 15-21.
http://dx.doi.org/10.1016/0166-6851(91)90216-S
---------- VANCOUVER ----------
Hellman, U., Wernstedt, C., Cazzulo, J.J. Self-proteolysis of the cysteine proteinase, cruzipain, from Trypanosoma cruzi gives a major fragment corresponding to its carboxy-terminal domain. Mol. Biochem. Parasitol. 1991;44(1):15-21.
http://dx.doi.org/10.1016/0166-6851(91)90216-S