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Abstract:

The molecular variants of Gonadotropin releasing hormone (GnRH) in brain extracts of the eutherian mammal Hydrochaeris hydrochaeris (Mammalia, Rodentia) were characterized. An indirect method combining reverse-phase high-performance liquid chromatography (RP-HPLC) and radioimmunoassay (RIA) with different antisera was used. Two different forebrain regions (olfactory bulbs and preoptic-hypothalamic region) were analyzed. Characterization of RP-HPLC fractions from preoptic-hypothalamic extracts with three different RIA systems revealed two immunoreactive GnRH (ir-GnRH) peaks coeluting with mammalian GnRH (mGnRH) and salmon GnRH (sGnRH) synthetic standards. These results were additionally supported by serial dilution studies with specific antisera. Similar results were obtained from olfactory bulb extracts with the same methodology. However, a third ir-GnRH peak in a similar position to that of chicken GnRH II (cIIGnRH) synthetic standard was revealed. As far as we know, this is the first report showing chromatographic and immunological evidences for the presence of a second GnRH variant in the forebrain of an eutherian mammal. Copyright (C) 1998 Elsevier Science B.V.

Registro:

Documento: Artículo
Título:Chromatographic and immunological identification of GnRH (Gonadotropin-releasing hormone) variants. Occurrence of mammalian and a salmon-like GnRH in the forebrain of an eutherian mammal: Hydrochaeris hydrochaeris (Mammalia, Rodentia)
Autor:Montaner, A.D.; Somoza, G.M.; King, J.A.; Bianchini, J.J.; Bolis, C.G.; Affanni, J.M.
Filiación:Inst. Neurociencia (INEUCI-CONICET), Depto. Biol., Fac. Cie. Exact. Y N., 1428 Buenos Aires, Argentina
Ctro. Invest. Cie. y T., Matteri y España 3105, Diamante, Entre Ríos, Argentina
Med. Res. Cncl. Regulatory P., Department of Chemical Pathology, Univ. of Cape Town Medical School, Observatory 7925, Cape Town, South Africa
Biología Generale, Istituto di Scienze Farmacologiche, Universitá di Milano, Via Balzarettim, 9-20133 Milan, Italy
Palabras clave:Capybara; GnRH; Gonadotropin-releasing hormone; HPLC; Mammalian; Radioimmunoassay; brain extract; gonadorelin; animal tissue; article; female; forebrain; hypothalamus; male; nonhuman; olfactory bulb; preoptic area; priority journal; reversed phase high performance liquid chromatography; rodent; Animals; Chromatography, High Pressure Liquid; Female; Gonadotropin-Releasing Hormone; Hypothalamus; Male; Mammals; Olfactory Bulb; Preoptic Area; Prosencephalon; Radioimmunoassay; Rodentia; Eutheria; Gallus gallus; Hydrochaeris hydrochaeris; Mammalia; Rodentia
Año:1998
Volumen:73
Número:3
Página de inicio:197
Página de fin:204
DOI: http://dx.doi.org/10.1016/S0167-0115(98)00005-6
Título revista:Regulatory Peptides
Título revista abreviado:Regul. Pept.
ISSN:01670115
CODEN:REPPD
CAS:Gonadotropin-Releasing Hormone, 33515-09-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01670115_v73_n3_p197_Montaner

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Citas:

---------- APA ----------
Montaner, A.D., Somoza, G.M., King, J.A., Bianchini, J.J., Bolis, C.G. & Affanni, J.M. (1998) . Chromatographic and immunological identification of GnRH (Gonadotropin-releasing hormone) variants. Occurrence of mammalian and a salmon-like GnRH in the forebrain of an eutherian mammal: Hydrochaeris hydrochaeris (Mammalia, Rodentia). Regulatory Peptides, 73(3), 197-204.
http://dx.doi.org/10.1016/S0167-0115(98)00005-6
---------- CHICAGO ----------
Montaner, A.D., Somoza, G.M., King, J.A., Bianchini, J.J., Bolis, C.G., Affanni, J.M. "Chromatographic and immunological identification of GnRH (Gonadotropin-releasing hormone) variants. Occurrence of mammalian and a salmon-like GnRH in the forebrain of an eutherian mammal: Hydrochaeris hydrochaeris (Mammalia, Rodentia)" . Regulatory Peptides 73, no. 3 (1998) : 197-204.
http://dx.doi.org/10.1016/S0167-0115(98)00005-6
---------- MLA ----------
Montaner, A.D., Somoza, G.M., King, J.A., Bianchini, J.J., Bolis, C.G., Affanni, J.M. "Chromatographic and immunological identification of GnRH (Gonadotropin-releasing hormone) variants. Occurrence of mammalian and a salmon-like GnRH in the forebrain of an eutherian mammal: Hydrochaeris hydrochaeris (Mammalia, Rodentia)" . Regulatory Peptides, vol. 73, no. 3, 1998, pp. 197-204.
http://dx.doi.org/10.1016/S0167-0115(98)00005-6
---------- VANCOUVER ----------
Montaner, A.D., Somoza, G.M., King, J.A., Bianchini, J.J., Bolis, C.G., Affanni, J.M. Chromatographic and immunological identification of GnRH (Gonadotropin-releasing hormone) variants. Occurrence of mammalian and a salmon-like GnRH in the forebrain of an eutherian mammal: Hydrochaeris hydrochaeris (Mammalia, Rodentia). Regul. Pept. 1998;73(3):197-204.
http://dx.doi.org/10.1016/S0167-0115(98)00005-6