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Mazzetti, M.B.; Tomio, J.M. "Characterization of porphobilinogen deaminase from rat liver" (1988) Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular. 957(1):97-104
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Abstract:

Porphobilinogen deaminase (porphobilinogen ammonia-lyase, EC 4.3.1.8) was isolated from rat liver. The final preparation was homogeneous according to polyacrylamide gel electrophoresis and immunodiffusion criteria. Electrophoresis of the native enzyme revealed a single band of activity which was distributed into three bands after incubation with porphobilinogen. When electrophoresed under denaturing condition it displayed a single polypeptide band with a molecular weight of 42 000 confirmed by exclusion chromatography and by sucrose density gradient centrifugattion. The enzyme showed a pH optimum of 7.5 both in 0.1 M sodium phosphate and 0.05 M Tris-HCl buffer, when assayed at 37°C. An isoelectric point of 4.9 for the native purified protein was found. Hepatic porphobilinogen deaminase was remarkably heat-stable showing maximum activity at 55-60°C with one break in the Arrhenius plot. The kinetic behaviour of the purified enzyme followed the typical Michaelis-Menten kinetics with values of Km = 17 μM and Vmax = 29.4 units power mg in 0.1 M phosphate buffer at 37°C. The amino acid composition was determined, showing that the enzyme had a low content of sulphur-containing amino acids and a considerable number of acidic residues per mol of polypeptide chain. Reagents known to interact with sulphydryl groups have small effect on rat liver enzyme activity. © 1988.

Registro:

Documento: Artículo
Título:Characterization of porphobilinogen deaminase from rat liver
Autor:Mazzetti, M.B.; Tomio, J.M.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires Argentina
Palabras clave:(Rat liver); Enzyme purification; Heme synthesis; Porphobilinogen deaminase characterization; liver enzyme; porphobilinogen deaminase; animal cell; electrophoresis; nonhuman; priority journal; rat; Amino Acids; Ammonia-Lyases; Animal; Hydrogen-Ion Concentration; Hydroxymethylbilane Synthase; Isoelectric Point; Kinetics; Liver; Macromolecular Systems; Molecular Weight; Protein Denaturation; Rats; Spectrum Analysis; Sulfhydryl Reagents; Support, Non-U.S. Gov't; Temperature
Año:1988
Volumen:957
Número:1
Página de inicio:97
Página de fin:104
DOI: http://dx.doi.org/10.1016/0167-4838(88)90161-6
Título revista:Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Título revista abreviado:Biochim. Biophys. Acta Protein Struct. Mol. Enzymol.
ISSN:01674838
CODEN:BBAED
CAS:porphobilinogen deaminase, 9036-47-9, 9074-91-3; Amino Acids; Ammonia-Lyases, EC 4.3.1.; Hydroxymethylbilane Synthase, EC 4.3.1.8; Macromolecular Systems; Sulfhydryl Reagents
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674838_v957_n1_p97_Mazzetti

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Citas:

---------- APA ----------
Mazzetti, M.B. & Tomio, J.M. (1988) . Characterization of porphobilinogen deaminase from rat liver. Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, 957(1), 97-104.
http://dx.doi.org/10.1016/0167-4838(88)90161-6
---------- CHICAGO ----------
Mazzetti, M.B., Tomio, J.M. "Characterization of porphobilinogen deaminase from rat liver" . Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular 957, no. 1 (1988) : 97-104.
http://dx.doi.org/10.1016/0167-4838(88)90161-6
---------- MLA ----------
Mazzetti, M.B., Tomio, J.M. "Characterization of porphobilinogen deaminase from rat liver" . Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, vol. 957, no. 1, 1988, pp. 97-104.
http://dx.doi.org/10.1016/0167-4838(88)90161-6
---------- VANCOUVER ----------
Mazzetti, M.B., Tomio, J.M. Characterization of porphobilinogen deaminase from rat liver. Biochim. Biophys. Acta Protein Struct. Mol. Enzymol. 1988;957(1):97-104.
http://dx.doi.org/10.1016/0167-4838(88)90161-6