Artículo

da Cruz Cabral, L.; Rodriguero, M.; Stenglein, S.; Fog Nielsen, K.; Patriarca, A."Characterization of small-spored Alternaria from Argentinean crops through a polyphasic approach" (2017) International Journal of Food Microbiology. 257:206-215
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Abstract:

Small-spored Alternaria have been isolated from a wide variety of food crops, causing both economic losses and human health risk due to the metabolites produced. Their taxonomy has been discussed widely, but no scientific consensus has been established in this field to date. Argentina is a major exporter of agricultural products, so it is essential to thoroughly understand the physiological behaviour of this pathogen in a food safety context. Thus, the objective of this work was to characterize small-spored Alternaria spp. obtained from tomato fruits, pepper fruits, wheat grains and blueberries from Argentina by a polyphasic approach involving metabolomic and phylogenetic analyses based on molecular and morphological characters. Morphological analysis divided the population studied into three groups; A. arborescens sp.-grp., A. tenuissima sp.-grp., and A. alternata sp.-grp. However, when these characters were simultaneously analysed with molecular data, no clearly separated groups were obtained. Haplotype network and phylogenetic analysis (both Bayesian and maximum parsimony) of a conserved region yielded the same result, suggesting that all isolates belong to the same species. Furthermore, no correlation could be established between morphological species-groups and a metabolite or group of metabolites synthesized. Thus, the whole set of analyses carried out in the present work supports the hypothesis that these small-spored Alternaria isolates from food belong to the same species. Identification at species level through classical morphology or modern molecular techniques does not seem to be a useful tool to predict toxicological risk in food matrices. The detection of any small-spored Alternaria from Section Alternaria (D.P. Lawr., Gannibal, Peever & B.M. Pryor 2013) in food implies a potential toxicological risk. © 2017 Elsevier B.V.

Registro:

Documento: Artículo
Título:Characterization of small-spored Alternaria from Argentinean crops through a polyphasic approach
Autor:da Cruz Cabral, L.; Rodriguero, M.; Stenglein, S.; Fog Nielsen, K.; Patriarca, A.
Filiación:Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Orgánica, Laboratorio de Microbiología de Alimentos, Buenos Aires, Argentina
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Ecología, Genética y Evolución, Instituto de Ecología, Genética y Evolución de Buenos Aires (IEGEBA - CONICET/UBA), Grupos de Investigación en Filogeografía y Filogenias Moleculares, Buenos Aires, Argentina
Laboratorio de Biología Funcional y Biotecnología (BIOLAB)-CICBA-INBIOTEC, CONICET, Área de Microbiología, Facultad de Agronomía, UNCPBA, Azul, Buenos Aires, Argentina
Technical University of Denmark, Department of Systems Biology, Kgs. Lyngby, Denmark
Palabras clave:Alternaria; Endopolygalacturonase gene; Food; Metabolites profile; Polyphasic approach; Alternaria; Argentina; Article; blueberry; Capsicum annuum; controlled study; food crop; food safety; grain; health hazard; lowbush blueberry; morphological trait; nonhuman; pest control; phylogeny; tomato; Alternaria; Bayes theorem; black pepper; classification; fruit; fungus spore; genetics; isolation and purification; microbiology; secondary metabolism; Vaccinium; vegetable; wheat; fungal DNA; polygalacturonase; Alternaria; Argentina; Bayes Theorem; Blueberry Plants; DNA, Fungal; Fruit; Lycopersicon esculentum; Phylogeny; Piper nigrum; Polygalacturonase; Secondary Metabolism; Spores, Fungal; Triticum; Vegetables
Año:2017
Volumen:257
Página de inicio:206
Página de fin:215
DOI: http://dx.doi.org/10.1016/j.ijfoodmicro.2017.06.026
Handle:http://hdl.handle.net/20.500.12110/paper_01681605_v257_n_p206_daCruzCabral
Título revista:International Journal of Food Microbiology
Título revista abreviado:Int. J. Food Microbiol.
ISSN:01681605
CODEN:IJFMD
CAS:polygalacturonase, 9023-92-1, 9032-75-1; DNA, Fungal; Polygalacturonase
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681605_v257_n_p206_daCruzCabral

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Citas:

---------- APA ----------
da Cruz Cabral, L., Rodriguero, M., Stenglein, S., Fog Nielsen, K. & Patriarca, A. (2017) . Characterization of small-spored Alternaria from Argentinean crops through a polyphasic approach. International Journal of Food Microbiology, 257, 206-215.
http://dx.doi.org/10.1016/j.ijfoodmicro.2017.06.026
---------- CHICAGO ----------
da Cruz Cabral, L., Rodriguero, M., Stenglein, S., Fog Nielsen, K., Patriarca, A. "Characterization of small-spored Alternaria from Argentinean crops through a polyphasic approach" . International Journal of Food Microbiology 257 (2017) : 206-215.
http://dx.doi.org/10.1016/j.ijfoodmicro.2017.06.026
---------- MLA ----------
da Cruz Cabral, L., Rodriguero, M., Stenglein, S., Fog Nielsen, K., Patriarca, A. "Characterization of small-spored Alternaria from Argentinean crops through a polyphasic approach" . International Journal of Food Microbiology, vol. 257, 2017, pp. 206-215.
http://dx.doi.org/10.1016/j.ijfoodmicro.2017.06.026
---------- VANCOUVER ----------
da Cruz Cabral, L., Rodriguero, M., Stenglein, S., Fog Nielsen, K., Patriarca, A. Characterization of small-spored Alternaria from Argentinean crops through a polyphasic approach. Int. J. Food Microbiol. 2017;257:206-215.
http://dx.doi.org/10.1016/j.ijfoodmicro.2017.06.026