Abstract:
In recent years, much attention has been paid to plant cell culture as a tool for the production of secondary metabolites and the expression of recombinant proteins. Plant cell immobilization offers many advantages for biotechnological processes. However, the most extended matrices employed, such as calcium-alginate, cannot fully protect entrapped cells. Sol-gel chemistry of silicates has emerged as an outstanding strategy to obtain biomaterials in which living cells are truly protected. This field of research is rapidly developing and a large number of bacteria and yeast-entrapping ceramics have already been designed for different applications. But even mild thermal and chemical conditions employed in sol-gel synthesis may result harmful to cells of higher organisms. Here we present a method for the immobilization of plant cells that allows cell growth at cavities created inside a silica matrix. Plant cell proliferation was monitored for a 6-month period, at the end of which plant calli of more than 1 mm in diameter were observed inside the inorganic host. The resulting hybrid device had good mechanical stability and proved to be an effective barrier against biological contamination, suggesting that it could be employed for long-term plant cell entrapment applications. © 2006 Elsevier B.V. All rights reserved.
Registro:
Documento: |
Artículo
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Título: | Plant cell proliferation inside an inorganic host |
Autor: | Perullini, M.; Rivero, M.M.; Jobbágy, M.; Mentaberry, A.; Bilmes, S.A. |
Filiación: | INQUIMAE-DQIAQF, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pab. II, C1428EHA Buenos Aires, Argentina Laboratorio de Agrobiotecnología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pab. II, C1428EHA Buenos Aires, Argentina
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Palabras clave: | Plant cell immobilization; Silica matrix; Sol-gel; Bacteria; Plant cell culture; Plants (botany); Silica; Sol-gels; Biological contamination; Plant cell immobilization; Silica matrix; Sol-gel chemistry; Biotechnology; analytic method; article; cell growth; cell proliferation; contamination; extracellular matrix; host; immobilized cell; microbial contamination; nonhuman; plant cell; priority journal; Cell Culture Techniques; Cell Proliferation; Cells, Immobilized; Phloem; Silicon Dioxide; Tobacco |
Año: | 2007
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Volumen: | 127
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Número: | 3
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Página de inicio: | 542
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Página de fin: | 548
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DOI: |
http://dx.doi.org/10.1016/j.jbiotec.2006.07.024 |
Título revista: | Journal of Biotechnology
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Título revista abreviado: | J. Biotechnol.
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ISSN: | 01681656
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CODEN: | JBITD
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CAS: | Silicon Dioxide, 7631-86-9
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v127_n3_p542_Perullini |
Referencias:
- Abranches, R., Marcel, S., Arcalis, E., Altmann, F., Fevereiro, P., Stoger, E., Plants as bioreactors: a comparative study suggests that Medicago truncatula is a promising production system (2005) J. Biotechnol., 120, pp. 121-134
- Boninsegna, S., Bosetti, P., Carturan, G., Dellagiacoma, G., Dal Monte, R., Rossi, M., Encapsulation of individual pancreatic islets by sol-gel SiO 2 : a novel procedure for perspective cellular grafts (2003) J. Biotechnol., 100, pp. 277-286
- Böttcher, H., Soltmann, U., Mertig, M., Pompe, W., Biocers: ceramics with incorporated microorganisms for biocatalytic, biosortive and functional materials development (2004) J. Mater. Chem., 14, pp. 2176-2188
- Bourgaud, F., Gravot, A., Milesi, S., Gontier, E., Production of plant secondary metabolites: a historical perspective (2001) Plant Sci., 161, pp. 839-851
- Davis, M.E., Ordered porous materials for emerging applications (2002) Nature, 417, pp. 813-820
- Dörnenburg, H., Evaluation of immobilization effects on metabolic activities and productivity in plant cell processes (2004) Process Biochem., 39, pp. 1369-1375
- Ferrer, M.L., Garcia-Carvajal, Z.Y., Yuste, L., Rojo, F., del Monte, F., Bacteria viability in sol-gel materials revisited: Cryo-SEM as a suitable tool to study the structural integrity of encapsulated bacteria (2006) Chem. Mater., 18 (6), pp. 1458-1463
- Hellwig, S., Drossard, J., Twyman, R.M., Fischer, R., Plant cell cultures for the production of recombinant proteins (2004) Nat. Biotechnol., 22, pp. 1415-1422
- Kataoka, K., Nagao, Y., Nukui, T., Akiyama, I., Tsuru, K., Hayakawa, S., Osaka, A., Huh, N., An organic-inorganic hybrid scaffold for the culture of HepG2 cells in a bioreactor (2005) Biomaterials, 26, pp. 2509-2516
- Kato, K., Matsumoto, T., Koiwai, S., Mizusaki, S., Nishida, K., Nogushi, M., Tamaki, E., Liquid suspension culture of tobacco cells (1972) Ferment Technology Today, pp. 689-695. , Terui G. (Ed), Society of Fermentation Technology, Osaka
- Kieran, P.M., MacLoughlin, P.F., Malone, D.M., Plant cell suspension cultures: some engineering considerations (1997) J. Biotechnol., 59, pp. 39-52
- Kuncova, G., Podrazky, O., Ripp, S., Trögl, J., Sayler, G.S., Demnerova, K., Vankova, R., Monitoring of the viability of cells immobilized by sol-gel process (2004) J. Sol-Gel Sci. Technol., 31, pp. 1-8
- Moon, K.H., Honda, H., Kobayashi, T., Development of a bioreactor suitable for embryogenic callus culture (1999) J. Biosci. Bioeng., 87, pp. 661-665
- Murashige, T., Skoog, F., A revised medium for rapid growth and bio assays with tobacco tissue cultures (1962) Physiol. Plant, 15, pp. 473-497
- Nagata, T., Nemoto, Y., Hasezawa, S., Tobacco BY-2 cell-line as the hela-cell in the cell biology of higher-plants (1992) Int. Rev. Cytol., 132, pp. 1-30
- Nassif, N., Coiffier, A., Coradin, T., Roux, C., Bouvet, O., Livage, J., Viability of bacteria in hybrid aqueous silica gels (2002) J. Sol-Gel Sci. Technol., 26, pp. 1-4
- Onishi, N., Sakamoto, Y., Hirosawa, T., Synthetic seed as an application of mass production of somatic embryos (1994) Plant Cell Tissue Organ Cult., 39, pp. 137-145
- Perullini, M., Jobbágy, M., Soller-Illia, G.J.A.A., Bilmes, S.A., Cellular growth at cavities created inside silica monoliths synthesized by sol-gel (2005) Chem. Mater., 17, pp. 3806-3808
- Ripp, S., Nievens, D.E., Ahn, Y., Werner, C., Jerrell, J., Eastern, J.P., Cox, C.D., Sayler, G.S., Controlled field release of a bioluminescent genetically engineered microorganism for bioremediation process monitoring and control (2000) Environ. Sci. Technol., 34, pp. 846-853
- Vaněk, T., Valterová, I., Vaňková, R., Vaisar, T., Biotransformation of (-)limonene using Solanum aviculare and Dioscorea deltoidea immobilized plant cells (1999) Biotechol. Lett., 21, pp. 625-628
- Vidinha, P., Augusto, V., Almeida, M., Fonseca, I., Fidalgo, A., Ilharco, L., Cabral, J.M.S., Barreiros, S., Sol-gel encapsulation: an efficient and versatile immobilization technique for cutinase in non-aqueous media (2006) J. Biotechnol., 121, pp. 23-33
- Wen Su, W., Jun He, B., Liang, H., Sun, S., A perfusion air-lift bioreactor for high density plant cell cultivation and secreted protein production (1996) J. Biotechnol., 50, pp. 225-233
Citas:
---------- APA ----------
Perullini, M., Rivero, M.M., Jobbágy, M., Mentaberry, A. & Bilmes, S.A.
(2007)
. Plant cell proliferation inside an inorganic host. Journal of Biotechnology, 127(3), 542-548.
http://dx.doi.org/10.1016/j.jbiotec.2006.07.024---------- CHICAGO ----------
Perullini, M., Rivero, M.M., Jobbágy, M., Mentaberry, A., Bilmes, S.A.
"Plant cell proliferation inside an inorganic host"
. Journal of Biotechnology 127, no. 3
(2007) : 542-548.
http://dx.doi.org/10.1016/j.jbiotec.2006.07.024---------- MLA ----------
Perullini, M., Rivero, M.M., Jobbágy, M., Mentaberry, A., Bilmes, S.A.
"Plant cell proliferation inside an inorganic host"
. Journal of Biotechnology, vol. 127, no. 3, 2007, pp. 542-548.
http://dx.doi.org/10.1016/j.jbiotec.2006.07.024---------- VANCOUVER ----------
Perullini, M., Rivero, M.M., Jobbágy, M., Mentaberry, A., Bilmes, S.A. Plant cell proliferation inside an inorganic host. J. Biotechnol. 2007;127(3):542-548.
http://dx.doi.org/10.1016/j.jbiotec.2006.07.024