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Abstract:

The present work was carried out with the aim to analyze the secretome of Trametes versicolor BAFC 2234 grown on tomato juice medium supplemented with copper and manganese. T. versicolor BAFC 2234 was selected among diverse wood dwelling agaricomycetes from Argentina by its ability to cause a strong white rot on hardwood and in addition to show high tolerance toward phenolic compounds. A considerable number of the identified proteins were related to the degradation/modification of lignocelluloses. Hydrolases, peroxidases and phenoloxidases were the most abundant enzymes produced under the above-mentioned culture conditions. The lignin-modifying oxidoreductases laccase, manganese peroxidase (MnP) and versatile peroxidase (VP) were successfully purified - the latter for the first time from T. versicolor. The native VP protein has a molecular mass of 45. kDa and an isoelectric point of pH 3.7. The study clearly shows that complex plant-based media being rich in phenolics, such as tomato juice, can stimulate the secretion of a broad set of extracellular lignocellulolytic enzymes. Using such natural products as fungal culture media may give the opportunity to investigate plant biomass decomposition as well as the biodegradation of organic pollutants in an environment close to nature. © 2013 Elsevier B.V.

Registro:

Documento: Artículo
Título:The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase
Autor:Carabajal, M.; Kellner, H.; Levin, L.; Jehmlich, N.; Hofrichter, M.; Ullrich, R.
Filiación:TU Dresden, International Institute Zittau, Department of Bio- and Environmental Sciences, Markt 23, 02763 Zittau, Germany
University of Buenos Aires, Faculty of Exact and Natural Sciences, Department of Biodiversity and Experimental Biology, Intendente Güiraldes 2160, Ciudad Universitaria, C1428EGA, Argentina
Interfaculty Institute for Genetics and Functional Genomics, Department of Functional Genomics, Ernst Moritz Arndt University of Greifswald, Greifswald, Germany
Palabras clave:Laccase; Manganese peroxidase; Secretome; Trametes versicolor; Versatile peroxidase; Laccases; Manganese peroxidase; Secretome; Trametes versicolor; Versatile peroxidase; Biodegradation; Fruits; Hardwoods; Lignin; Proteins; Purification; Enzymes; copper; fungal protein; hydrolase; laccase; lignocellulose; manganese; manganese peroxidase; monophenol monooxygenase; peroxidase; phenol derivative; secretome; unclassified drug; Argentina; article; carbon source; culture medium; degradation; elution; enzyme purification; enzyme synthesis; extracellular space; fruit juice; isoelectric point; molecular weight; nonhuman; pH; polyacrylamide gel electrophoresis; priority journal; protein analysis; protein secretion; tomato; Trametes versicolor; Biodegradation; Enzymes; Fruits; Hardwoods; Laccase; Lignins; Proteins; Purification; Laccase; Manganese peroxidase; Secretome; Trametes versicolor; Versatile peroxidase; Culture Media; Hydrolases; Lycopersicon esculentum; Monophenol Monooxygenase; Oxidoreductases; Peroxidase; Trametes
Año:2013
Volumen:168
Número:1
Página de inicio:15
Página de fin:23
DOI: http://dx.doi.org/10.1016/j.jbiotec.2013.08.007
Título revista:Journal of Biotechnology
Título revista abreviado:J. Biotechnol.
ISSN:01681656
CODEN:JBITD
CAS:copper, 15158-11-9, 7440-50-8; hydrolase, 9027-41-2; laccase, 80498-15-3; lignocellulose, 11132-73-3; manganese, 16397-91-4, 7439-96-5; manganese peroxidase, 114995-15-2; monophenol monooxygenase, 9002-10-2; peroxidase, 9003-99-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v168_n1_p15_Carabajal

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Citas:

---------- APA ----------
Carabajal, M., Kellner, H., Levin, L., Jehmlich, N., Hofrichter, M. & Ullrich, R. (2013) . The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase. Journal of Biotechnology, 168(1), 15-23.
http://dx.doi.org/10.1016/j.jbiotec.2013.08.007
---------- CHICAGO ----------
Carabajal, M., Kellner, H., Levin, L., Jehmlich, N., Hofrichter, M., Ullrich, R. "The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase" . Journal of Biotechnology 168, no. 1 (2013) : 15-23.
http://dx.doi.org/10.1016/j.jbiotec.2013.08.007
---------- MLA ----------
Carabajal, M., Kellner, H., Levin, L., Jehmlich, N., Hofrichter, M., Ullrich, R. "The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase" . Journal of Biotechnology, vol. 168, no. 1, 2013, pp. 15-23.
http://dx.doi.org/10.1016/j.jbiotec.2013.08.007
---------- VANCOUVER ----------
Carabajal, M., Kellner, H., Levin, L., Jehmlich, N., Hofrichter, M., Ullrich, R. The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase. J. Biotechnol. 2013;168(1):15-23.
http://dx.doi.org/10.1016/j.jbiotec.2013.08.007