Artículo

Delfosse, V.C.; Agrofoglio, Y.C.; Casse, M.F.; Kresic, I.B.; Hopp, H.E.; Ziegler-Graff, V.; Distéfano, A.J. "The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing" (2014) Virus Research. 180:70-75
El editor solo permite decargar el artículo en su versión post-print desde el repositorio. Por favor, si usted posee dicha versión, enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Plants employ RNA silencing as a natural defense mechanism against viruses. As a counter-defense, viruses encode silencing suppressor proteins (SSPs) that suppress RNA silencing. Most, but not all, the P0 proteins encoded by poleroviruses have been identified as SSP. In this study, we demonstrated that cotton leafroll dwarf virus (CLRDV, genus Polerovirus) P0 protein suppressed local silencing that was induced by sense or inverted repeat transgenes in Agrobacterium co-infiltration assay in Nicotiana benthamiana plants. A CLRDV full-length infectious cDNA clone that is able to infect N. benthamiana through Agrobacterium-mediated inoculation also inhibited local silencing in co-infiltration assays, suggesting that the P0 protein exhibits similar RNA silencing suppression activity when expressed from the full-length viral genome. On the other hand, the P0 protein did not efficiently inhibit the spread of systemic silencing signals. Moreover, Northern blotting indicated that the P0 protein inhibits the generation of secondary but not primary small interfering RNAs. The study of CLRDV P0 suppression activity may contribute to understanding the molecular mechanisms involved in the induction of cotton blue disease by CLRDV infection. © 2013 Elsevier B.V.

Registro:

Documento: Artículo
Título:The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing
Autor:Delfosse, V.C.; Agrofoglio, Y.C.; Casse, M.F.; Kresic, I.B.; Hopp, H.E.; Ziegler-Graff, V.; Distéfano, A.J.
Filiación:Instituto de Biotecnología, CICVyA, CNIA, INTA, Buenos Aires, Argentina
EEA Saenz Peña, INTA, Chaco, Argentina
Facultad de Ciencias Exactas y Naturales, UBA, Buenos Aires, Argentina
Institut de Biologie Moléculaire des Plantes, Laboratoire propre du CNRS conventionné avec l'Université de Strasbourg, Strasbourg, France
Idioma: Inglés
Palabras clave:CLRDV; P0 protein; Polerovirus; Silencing suppressor
Año:2014
Volumen:180
Página de inicio:70
Página de fin:75
DOI: http://dx.doi.org/10.1016/j.virusres.2013.12.018
Título revista:Virus Research
Título revista abreviado:Virus Res.
ISSN:01681702
CODEN:VIRED
Registro:http://digital.bl.fcen.uba.ar/collection/paper/document/paper_01681702_v180_n_p70_Delfosse

Referencias:

  • Baumberger, N., Tsai, C.H., Lie, M., Havecker, E., Baulcombe, D.C., The polerovirus silencing suppressor P0 targets ARGONAUTE proteins for degradation (2007) Curr. Biol., 17, pp. 1609-1614
  • Bortolamiol, D., Pazhouhandeh, M., Marrocco, K., Genschik, P., Ziegler-Graff, V., The polerovirus F box protein P0 targets ARGONAUTE1 to suppress RNA silencing (2007) Curr. Biol., 17, pp. 1615-1621
  • Brigneti, G., Voinnet, O., Li, W.X., Ji, L.H., Ding, S.W., Boulcombe, D.C., Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana (1998) EMBO J., 17, pp. 6739-6746
  • Corrêa, R.L., Silva, T.F., Simões-Araújo, J.L., Barroso, P.A., Vidal, M.S., Vaslin, M.F.S., Molecular characterization of a virus from the family Luteoviridae associated with cotton blue disease (2005) Arch. Virol., 50, pp. 1357-1367
  • Deleris, A., Gallego-Bartolome, J., Bao, J., Kasschau, K.D., Carrington, J.C., Voinnet, O., Hierarchical action and inhibition of plant Dicer-like proteins in antiviral defense (2006) Science, 313, pp. 68-71
  • Delfosse, V.C., Casse, M.F., Agrofoglio, Y.C., Bonacic Kresic, I., Hopp, H.E., Ziegler-Graff, V., Distéfano, A.J., Agroinoculation of a full-length cDNA clone of cotton leafroll dwarf virus (CLRDV) results in systemic infection in cotton and the model plant Nicotiana benthamiana (2013) Virus Res., 175, pp. 64-70
  • Derrien, B., Baumberger, N., Schepetilnikov, M., Viotti, C., De Cillia, J., Ziegler-Graff, V., Isono, E., Genschik, P., Degradation of the antiviral component ARGONAUTE1 by the autophagy pathway (2012) Proc. Natl. Acad. Sci. U.S.A., 109, pp. 15942-15946
  • Distéfano, A.J., Bonacic Kresic, I., Hopp, H.E., The complete genome sequence of a virus associated with cotton blue disease, cotton leafroll dwarf virus, confirms that it is a new member of the genus Polerovirus (2010) Arch. Virol., 155, pp. 1849-1854
  • Fusaro, A.F., Corrêa, R.L., Nakasugi, K., Jackson, C., Kawchuk, L., Vaslin, M.F.S., Waterhouse, P.M., The Enamovirus P0 protein is a silencing suppressor which inhibits local and systemic RNA silencing through AGO1 degradation (2012) Virology, 426, pp. 178-187
  • Hamilton, A.J., Baulcombe, D.C., A novel species of small antisense RNA in post-transcriptional gene silencing (1999) Science, 286, pp. 950-952
  • Hamilton, C.M., Frary, A., Lewis, C., Tanksley, S.D., Stable transfer of intact high molecular weight DNA into plant chromosomes (1996) Proc. Natl. Acad. Sci. U.S.A., 93, pp. 9975-9979
  • Han, Y.H., Xiang, H.Y., Wang, Q., Li, Y.Y., Wu, W.Q., Han, C.G., Li, D.W., Yu, J.L., Ring structure amino acids affect the suppressor activity of melon aphid-borne yellows virus P0 protein (2010) Virology, 406, pp. 21-27
  • Himber, C., Dunoyer, P., Moissiard, G., Ritzenthaler, C., Voinnet, O., Transitivity-dependent and -independent cell-to-cell movement of RNA silencing (2003) EMBO J., 22, pp. 4523-4533
  • Incarbone, M., Dunoyer, P., RNA silencing and its suppression: novel insights from in planta analyses (2013) Trends Plant Sci., 18, pp. 382-392
  • King, A.M.Q., Adams, M.J., Carstens, E.B., Lefkowitz, E.J., (2011) Viral Taxonomy. Ninth Report of the International Committee on Taxonomy of Viruses (ICTV), , Elsevier Academic Press, San Diego
  • Klein, E., Brault, V., Klein, D., Weyens, G., Lefèbvre, M., Ziegler-Graff, V., Gilmer, D., Divergence of host range and biological properties between natural isolate and full-length infectious cDNA clone of the Beet mild yellowing virus 2ITB (2014) Mol. Plant Pathol., 15, pp. 22-30
  • Kozlowska-Makulska, A., Guilley, H., Szyndel, M.S., Beuve, M., Lemaire, O., Herrbach, E., Bouzoubaa, S., P0 proteins of European beet-infecting poleroviruses display variable RNA silencing suppression activity (2010) J. Gen. Virol., 91, pp. 1082-1091
  • Liu, Y., Zhai, H., Zhao, K., Wu, B., Wang, X., Two suppressors of RNA silencing encoded by cereal-infecting members of the family Luteoviridae (2012) J. Gen. Virol., 93, pp. 1825-1830
  • Lu, R., Folimonov, A., Shintaku, M., Li, W.X., Falk, B.W., Dawson, W.O., Ding, S.W., Three distinct suppressors of RNA silencing encoded by a 20-kb viral genome (2004) Proc. Natl. Acad. Sci. U.S.A., 101, pp. 15742-15747
  • Llave, C., Virus-derived small interfering RNAs at the core of plant-virus interactions (2010) Trends Plant Sci., 15, pp. 701-707
  • Mangwende, T., Wang, M.L., Borth, W., Hu, J., Moore, P.H., Mirkov, T.E., Albert, H.H., The P0 gene of sugarcane yellow leaf virus encodes an RNA silencing suppressor with unique activities (2009) Virology, 384, pp. 38-50
  • Mayo, M.A., Miller, W.A., The structure and expression of luteovirus genomes (1999) The Luteoviridae, pp. 23-42. , CABI Publishing, Wallingford, CT, H.G. Smith, H. Barker (Eds.)
  • Pazhouhandeh, M., Dieterle, M., Marrocco, K., Lechner, E., Berry, B., Brault, V., Hemmer, O., Genschik, P., F-box-like domain in the polerovirus protein P0 is required for silencing suppressor function (2006) Proc. Natl. Acad. Sci. U.S.A., 103, pp. 1994-1999
  • Pfeffer, S., Dunoyer, P., Heim, F., Richards, K.E., Jonard, G., Ziegler-Graff, V., P0 of beet western yellows virus is a suppressor of posttranscriptional gene silencing (2002) J. Virol., 76, pp. 6815-6824
  • Romanel, E., Silva, T.F., Corrêa, R.L., Farinelli, L., Hawkins, J.S., Schrago, C.E., Vaslin, M.F., Global alteration of microRNAs and transposon-derived small RNAs in cotton (Gossypium hirsutum) during Cotton leafroll dwarf polerovirus (CLRDV) infection (2012) Plant Mol. Biol., 80, pp. 443-460
  • Voinnet, O., Vain, P., Angell, S., Baulcombe, D.C., Systemic spread of sequence-specific transgene RNA degradation is initiated by localized introduction of ectopic promoterless DNA (1998) Cell, 95, pp. 177-187
  • Voinnet, O., Lederer, C., Boulcombe, D.C., A viral movement protein prevents spread of the gene silencing in Nicotiana benthamiana (2000) Cell, 103, pp. 157-167
  • Voinnet, O., Induction and suppression of RNA silencing: insights from viral infections (2005) Nat. Rev. Genet., 6, pp. 206-220

Citas:

---------- APA ----------
Delfosse, V.C., Agrofoglio, Y.C., Casse, M.F., Kresic, I.B., Hopp, H.E., Ziegler-Graff, V. & Distéfano, A.J. (2014) . The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing. Virus Research, 180, 70-75.
http://dx.doi.org/10.1016/j.virusres.2013.12.018
---------- CHICAGO ----------
Delfosse, V.C., Agrofoglio, Y.C., Casse, M.F., Kresic, I.B., Hopp, H.E., Ziegler-Graff, V., et al. "The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing" . Virus Research 180 (2014) : 70-75.
http://dx.doi.org/10.1016/j.virusres.2013.12.018
---------- MLA ----------
Delfosse, V.C., Agrofoglio, Y.C., Casse, M.F., Kresic, I.B., Hopp, H.E., Ziegler-Graff, V., et al. "The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing" . Virus Research, vol. 180, 2014, pp. 70-75.
http://dx.doi.org/10.1016/j.virusres.2013.12.018
---------- VANCOUVER ----------
Delfosse, V.C., Agrofoglio, Y.C., Casse, M.F., Kresic, I.B., Hopp, H.E., Ziegler-Graff, V., et al. The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing. Virus Res. 2014;180:70-75.
http://dx.doi.org/10.1016/j.virusres.2013.12.018