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Abstract:

Poor-quality sperm show reduced capacity to under-go capacitation-induced protein tyrosine phosphorylation and hyperactivation. Given that these deficiencies can be overcome by membrane-permeant stimulators of the cAMP-dependent kinase system, we hypothesize that the main defect underlying these deficiencies resides on the sperm plasma membrane. Spermatozoa from semen samples obtained from 15 consenting healthy donors were separated in 2 subpopulations, L45 (first interface) and L90 (pellet), using a 45:65:90 isolate gradient centrifugation method. These sperm fractions were studied before and after a 6-hour capacitating incubation for sperm motion parameters (computer-assisted analysis), including hyperactivation, protein tyrosine phos-phorylation (immunofluorescence), membrane fluidity (Laurdan fluorescence), and sterol and phospholipid content (high-performance thin-layer chromatography). In summary, data indicate that L45 (poor-motility) spermatozoa present an excess of cholesterol and desmosterol, which impairs the normal increase in membrane fluidity during capacitation and its consequent activation of protein tyrosine phosphorylation and hypermotility. Therefore, a defect in membrane composition and dynamics is underlying human sperm biochemical and functional deficiencies related to inadequate capacitation. Copyright © American Society of Andrology.

Registro:

Documento: Artículo
Título:High cholesterol content and decreased membrane fluidity in human spermatozoa are associated with protein tyrosine phosphorylation and functional deficiencies
Autor:Buffone, M.G.; Verstraeten, S.V.; Calamera, J.C.; Doncel, G.F.
Filiación:University of Pennsylvania, Center for Research on Reproduction and Women's Health, Philadelphia, PA, United States
Departamento de Química Biológica, IIMHNO (UBA) and IQUIFIB, Universidad de Buenos Aires, Buenos Aires, Argentina
Laboratorio de Estudios en Reproducción (LER), Buenos Aires, Argentina
CONRAD Program, Department of OB/GYN, Eastern Virginia Medical School, 601 Colley Avenue, Norfolk, VA 23507, United States
Palabras clave:Capacitation; Hyperactivation; Plasma membrane; cholesterol; desmosterol; article; cell membrane; controlled study; human; human experiment; male; membrane fluidity; normal human; priority journal; protein phosphorylation; seminal plasma; spermatozoon; spermatozoon capacitation; spermatozoon motility; Cell Membrane; Cholesterol; Desmosterol; Humans; Male; Membrane Fluidity; Phosphorylation; Sperm Capacitation; Sperm Motility; Spermatozoa; Tyrosine
Año:2009
Volumen:30
Número:5
Página de inicio:552
Página de fin:558
DOI: http://dx.doi.org/10.2164/jandrol.108.006551
Título revista:Journal of Andrology
Título revista abreviado:J. Androl.
ISSN:01963635
CODEN:JOAND
CAS:cholesterol, 57-88-5; desmosterol, 313-04-2; Cholesterol, 57-88-5; Desmosterol, 313-04-2; Tyrosine, 55520-40-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01963635_v30_n5_p552_Buffone

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Citas:

---------- APA ----------
Buffone, M.G., Verstraeten, S.V., Calamera, J.C. & Doncel, G.F. (2009) . High cholesterol content and decreased membrane fluidity in human spermatozoa are associated with protein tyrosine phosphorylation and functional deficiencies. Journal of Andrology, 30(5), 552-558.
http://dx.doi.org/10.2164/jandrol.108.006551
---------- CHICAGO ----------
Buffone, M.G., Verstraeten, S.V., Calamera, J.C., Doncel, G.F. "High cholesterol content and decreased membrane fluidity in human spermatozoa are associated with protein tyrosine phosphorylation and functional deficiencies" . Journal of Andrology 30, no. 5 (2009) : 552-558.
http://dx.doi.org/10.2164/jandrol.108.006551
---------- MLA ----------
Buffone, M.G., Verstraeten, S.V., Calamera, J.C., Doncel, G.F. "High cholesterol content and decreased membrane fluidity in human spermatozoa are associated with protein tyrosine phosphorylation and functional deficiencies" . Journal of Andrology, vol. 30, no. 5, 2009, pp. 552-558.
http://dx.doi.org/10.2164/jandrol.108.006551
---------- VANCOUVER ----------
Buffone, M.G., Verstraeten, S.V., Calamera, J.C., Doncel, G.F. High cholesterol content and decreased membrane fluidity in human spermatozoa are associated with protein tyrosine phosphorylation and functional deficiencies. J. Androl. 2009;30(5):552-558.
http://dx.doi.org/10.2164/jandrol.108.006551