Inositol phospholipids (IPL) from epimastigote forms of Trypanosoma cruzi have been investigated by metabolic labelling with [3H]palmitic acid and by GLC-MS analysis of the lipids obtained from non-labelled parasites. The IPL fraction was separated into phosphatidylinositol (PI) and inositol-phosphoceramide subfractions, the latter accounting for 80-85% of the total IPL. The neutral lipids released from the IPLs by PI-specific phospholipase C (PI-PLC) from Bacillus thuringiensis were analysed by silica-gel and reverse-phase TLC for the radioactive lipids and by GLC-MS for the non-radioactive samples. Ceramides containing dihydrosphingosine and sphingosine with C(16:0) and C(18:0) fatty acids were identified. The main component in the [3H]palmitic acid-labelled ceramides was palmitoyldihydrosphingosine, while in the non-labelled sample the ceramides contained mainly sphingosine. This could reflect partial uptake of phospholipid from the medium. The PI contain both alkylacyl- and diacyl-glycerol lipids, with the ether lipid being more abundant. The latter was identified as 1-O-hexadecylglycerol esterified by C(18:2) and C(18:1) fatty acids. Interestingly, the same lipid had been identified in the anchor of the 1G7 glycoprotein of T. cruzi metacyclic forms.
Documento: | Artículo |
Título: | Structural analysis of inositol phospholipids from Trypanosoma cruzi epimastigote forms |
Autor: | Bertello, L.E.; Goncalvez, M.F.; Colli, W.; De Lederkremer, R.M. |
Filiación: | Departamento de Quimica Organica, Facultad Ciencias Exactas Naturales, Universidad de Buenos Aires, Pabellon 2, 1428 Buenos Aires, Argentina |
Palabras clave: | ceramide; diacylglycerol; fatty acid; glycoprotein; palmitic acid; phosphatidylinositide; phosphatidylinositol; phospholipase C; sphinganine; sphingosine; article; Bacillus thuringiensis; esterification; gas liquid chromatography; lipid analysis; mass spectrometry; nonhuman; priority journal; thin layer chromatography; Trypanosoma cruzi; Bacillus thuringiensis; Trypanosoma; Trypanosoma cruzi |
Año: | 1995 |
Volumen: | 310 |
Número: | 1 |
Página de inicio: | 255 |
Página de fin: | 261 |
DOI: | http://dx.doi.org/10.1042/bj3100255 |
Título revista: | Biochemical Journal |
Título revista abreviado: | BIOCHEM. J. |
ISSN: | 02646021 |
CODEN: | BIJOA |
CAS: | palmitic acid, 57-10-3; phospholipase C, 9001-86-9; sphinganine, 764-22-7; sphingosine, 123-78-4 |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v310_n1_p255_Bertello |