Abstract:
The influence of hydroxypropylmethycellulose (HPMC) on whey protein (WPC) gelling properties was studied under thermodynamic incompatibility conditions at neutral pH and room temperature. The binodal curve characterizing phase separation was determined. Thermal transitions of mixed systems above the binodal curve showed two endothermic peaks corroborating phase separation. First of all the gelation process of single HPMC was studied by different techniques in order to interpret the behaviour of mixed systems. Gelation dynamics of mixed systems showed two competitive phenomena during heating, phase separation and gelation. The solid character of mixtures increased when WPC concentration was higher than 8% and the WPC/HPMC ratio was 4 or higher. The gelation temperature, determined by dynamic rheometry, was related to the relative amount of both components. The morphology of mixed gels after the heating/cooling cycle was of the type core-shell, where the core was constituted by HPMC gel. The elastic modulus of mixed gels was much higher than that of single WPC gels at the same concentration, while hardness and relative viscoelasticity were slightly decreased. Core-shell macrostructure results are very interesting because it could find applications in the design of microcapsules or in controlled delivery systems, in which an active component could be partitioned into separated phases or to develop new structures and food products. © 2005 Elsevier Ltd. All rights reserved.
Registro:
Documento: |
Artículo
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Título: | Gelation and structural characteristics of incompatible whey proteins/hydroxypropylmethylcellulose mixtures |
Autor: | Pérez, O.E.; Wargon, V.; M.R. Pilosof, A. |
Filiación: | Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires. Ciudad Universitaria, 1428 Buenos Aires, Argentina Universidad de Buenos Aires, Buenos Aires, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina
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Palabras clave: | Core-shell gels; Delivery; Gelation; Hydroxypropylmethylcellulose; Phase separation; Whey proteins |
Año: | 2006
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Volumen: | 20
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Número: | 7
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Página de inicio: | 966
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Página de fin: | 974
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DOI: |
http://dx.doi.org/10.1016/j.foodhyd.2005.11.005 |
Título revista: | Food Hydrocolloids
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Título revista abreviado: | Food Hydrocolloids
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ISSN: | 0268005X
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CODEN: | FOHYE
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0268005X_v20_n7_p966_Perez |
Referencias:
- Baeza, R.I., Carp, D.J., Pérez, O.E., Pilosof, A.M.R., κ-Carrageenan-protein interactions: Effect of proteins on polysaccharide gelling and textural properties (2002) Lebensmittel-Wissenschaft und-Technologie, 35, pp. 741-747
- Baeza, R.I., Pilosof, A.M.R., Mixed biopolymer gel systems of β-lactoglobulin and non-gelling gums (2001) Food colloids, fundamentals of formulation, pp. 392-403. , Dickinson E., and Miller R. (Eds), RSC, Cambridge
- Baeza, R.I., Pilosof, A.M.R., Calorimetric studies of thermal denaturation of β-lactoglobulin in the presence of polysaccharides (2002) Lebensmittel-Wissenschaft und-Technologie, 35, pp. 393-399
- Beaulieu, C.M., Turgeon, S.L., Doublier, J.L., Rheology, texture and microstructure of whey proteins/low methoxyl pectins mixed gels with added calcium (2001) International Dairy Journal, 11, pp. 961-967
- Capron, I., Nicolai, T., Smith, C., Effect of addition of κ-carrageenan on the mechanical and structural properties of β-lactoglobulin gels (1999) Carbohydrates Polymers, 40, pp. 233-238
- Cayot, P., Lorient, D., Structure-function relationships of whey proteins (1997) Food proteins and their applications, pp. 225-256. , Damodaran S., and Paraf A. (Eds), Marcel Dekker, New York
- Chevillard, C., Axelos, M.A.V., Phase separation of aqueous solution of methylcellulose (1997) Colloid and Polymer Science, 275, pp. 537-545
- Dickinson, E., Emulsion stabilization by polysaccharides and protein-polysaccharide complexes (1995) Food polysaccharides and their application, pp. 501-515. , Stephen A.M. (Ed), Marcel Dekker Inc, New York
- Doublier, J.L., Garnier, C., Renard, D., Sánchez, C., Protein-polysaccharide interactions (2000) Current Opinion in Colloids & Interface Science, 5, pp. 202-214
- Eleya, M.M.O., Turgeon, S.L., Rheology of κ-carrageenan and β-lactoglobulin mixed gels (2000) Food Hydrocolloids, 14, pp. 29-40
- Feldstein, M.M., Roos, A., Chevallier, C., Creton, C., Dormidontova, E.E., Relation of glass transition temperature to the hydrogen bonding degree and energy in poly(N-vinyl pyrrolidone) blends with hydroxyl-containing plasticizers: 3. Analysis of two glass transition temperatures featured for PVP solutions in liquid poly(ethylene glycol) (2003) Polymer, 44, pp. 1819-1834
- Fernandes, P., Viscoelastic characteristics of whey protein systems at neutral pH (1994) Food Hydrocolloids, 8, pp. 277-285
- Ford, J.L., Thermal analysis of hydroxypropylmethycellulose and methylcellulose: powders, gels and matrix tables (1999) International Journal of Pharmaceutics, 179, pp. 209-228
- Ford, J.L., Mitchell, K., Thermal analysis of gels and matrix tablets containing cellulose ethers (1995) Thermochimica Acta, 248, pp. 329-345
- Fyfe, C.A., Blazek, A.I., Investigation of hydrogel formation from hydroxypropylmethylcellulose (HPMC) by NMR spectroscopy and NMR imaging techniques (1997) Macromolecules, 30, pp. 6230-6237
- Galazka, V.B., Smith, D., Ledward, D.A., Dickinson, E., Interactions of bovine serum albumin with sulphated polysacchaides: Effcts of pH ionic strength and high pressure treatment (1999) Food Chemistry, 64, pp. 303-310
- Hemar, Y., Tamehana, M., Munro, P.A., Singh, H., Viscosity, microstructure and phase behavior of aqueous mixtures of commercial milk protein products and xanthan gum (2001) Food Hydrocolloids, 15, pp. 565-574
- Hoffmann, M.A.M., Van Mil, P.J.J.M., Heat-induced aggregation of β-lactoglobulin: As a function of pH (1999) Journal of Agricultural and Food Chemistry, 47, pp. 1898-1905
- Kato, T., Yokoyama, M., Takahashi, A., Melting temperatures of thermally reversible gels IV. Methyl cellulose-water gels (1978) Colloid and Polymer Science, 266, pp. 15-21
- Kobayashi, K., Huang, C., Lodge, T.P., Thermorreversible gelation of aqueous methylcellulose solutions (1999) Macromolecules, 32, pp. 7070-7077
- Lorén, N., Hermansson, A.M., Structure evolution during phase separation and gelation of biopolymer mixtures (2003) Food colloids, biopolymers and materials, pp. 298-308. , Dickinson E., and Van Vliet T. (Eds), RSC, Cambridge
- Mc Cristal, C.B., Ford, J.L., Rajabi-Siahboomi, A.R., A study on the interaction of water and cellulose ethers using differential scanning calorimetry (1997) Thermochimica Acta, 294, pp. 91-98
- Mulvihill, D.M., Donovan, M., Whey proteins and their thermal denaturation. A review (1987) Irish Journal of Food Science and Technology, 11, pp. 43-75
- Norton, I.T., Frith, W.J., Phase separation in mixed biopolymer systems (2003) Food colloids, biopolymers and materials, pp. 282-297. , Dickinson E., and Van Vliet T. (Eds), RSC, Cambridge
- Puyol, P., Perez, M.D., Horne, D.S., Heat-induced gelation of whey protein isolates (WPI): Effect of NaCl and protein concentration (2001) Food Hydrocolloids, 15, pp. 233-237
- Relkin, P., Meylheuc, T., Launay, B., Raynal, K., Heat-induced gelation of globular protein mixtures. A DSC and scanning electron microscopic study (1998) Journal of Thermal Analysis, 51, pp. 747-755
- Sarkar, N., Kinetics of thermal gelation of methylcellulose and hydroxypropylmethycellulose in aqueous solutions (1995) Carbohydrate Polymer, 26, pp. 195-203
- Sarkar, N., Walker, L.C., Hydration-dehydration properties of methylcellulose and hydroxypropylmethylcellulose (1995) Carbohydrate Polymers, 27, pp. 177-185
- Schorsch, C., Jones, M.G., Norton, I.T., Thermodynamic incompatibility and microstructure of milk protein/locust bean gum/sucrose systems (1999) Food Hydrocolloids, 13, pp. 89-99
- Tanaka, F., Phase formation of associating polymers: gelation, phase separation and microphase formation (1996) Advances in Colloid and Interface Science, 63, pp. 23-40
- Tavares, C., Lopes da Silva, J.A., Rheology of galactomannan-whey protein mixed systems (2003) International Dairy Journal, 13, pp. 699-706
- Tolstoguzov, V.B., Some physico-chemical aspects of protein processing in foods. Multicomponent gels (1995) Food Hydrocolloids, 9 (4), pp. 317-332
- Tolstoguzov, V., Some thermodynamic considerations in food formulation (2003) Food Hydrocolloids, 17, pp. 1-23
- Tritt-Goc, J., Pislewski, N., Magnetic resonance imaging study of the swelling kinetics of hydroxypropylmethylcellulose (HPMC) in water (2002) Journal of Controlled Release, 80, pp. 79-86
- Turgeon, S.L., Beaulieu, M., Schmitt, C., Sánchez, C., Protein-polysaccharide interactions: phase-ordering kinetics, thermodynamic and structural aspects (2003) Current Opinion in Colloid and Interface Science, 8, pp. 401-414
- Verheul, M., Pedersen, J.S., Roefs, S.P.F.M., de Kruif, K.G., Association behavior of native β-lactoglobulin (1999) Biopolymers, 49, pp. 11-20
- Yuguchi, Y., Urakawa, H., Kitamura, S., Ohno, S., Kajiwara, K., Gelation mechanism of methylhydroxypropylcellulose in aqueous solution (1995) Food Hydrocolloids, 9 (3), pp. 173-179
- Zhang, G., Foegeding, E.A., Heat-induced phase behaviour of β-lactoglobulin/polysaccharide mixtures (2003) Food Hydrocolloids, 17, pp. 785-792
- Zhuravskaya, N.A., Kiknadze, E.V., Antonov, Y.A., Tolstoguzov, V.B., (1986) Nahrung, 30. , 591-599; 601-613
Citas:
---------- APA ----------
Pérez, O.E., Wargon, V. & M.R. Pilosof, A.
(2006)
. Gelation and structural characteristics of incompatible whey proteins/hydroxypropylmethylcellulose mixtures. Food Hydrocolloids, 20(7), 966-974.
http://dx.doi.org/10.1016/j.foodhyd.2005.11.005---------- CHICAGO ----------
Pérez, O.E., Wargon, V., M.R. Pilosof, A.
"Gelation and structural characteristics of incompatible whey proteins/hydroxypropylmethylcellulose mixtures"
. Food Hydrocolloids 20, no. 7
(2006) : 966-974.
http://dx.doi.org/10.1016/j.foodhyd.2005.11.005---------- MLA ----------
Pérez, O.E., Wargon, V., M.R. Pilosof, A.
"Gelation and structural characteristics of incompatible whey proteins/hydroxypropylmethylcellulose mixtures"
. Food Hydrocolloids, vol. 20, no. 7, 2006, pp. 966-974.
http://dx.doi.org/10.1016/j.foodhyd.2005.11.005---------- VANCOUVER ----------
Pérez, O.E., Wargon, V., M.R. Pilosof, A. Gelation and structural characteristics of incompatible whey proteins/hydroxypropylmethylcellulose mixtures. Food Hydrocolloids. 2006;20(7):966-974.
http://dx.doi.org/10.1016/j.foodhyd.2005.11.005