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Abstract:

Soybean callus δ-aminolaevulinate synthetase (ALA-S) has been covalently attached to Sepharose 4B. The optimal conditions for binding have been determined. The water-insoluble ALA-S retained 40% of the activity of the original soluble preparation, the coupling yield was also high. Sepharose - ALA-S could be stored at 4°C for periods up to 40 days with only 25% loss of activity and it could be repeatedly used with little alteration of its enzymic activity. pH optima of the free and bound enzyme were the same. © 1978.

Registro:

Documento: Artículo
Título:Porphyrin biosynthesis-immobilized enzymes and ligands. IX. Studies on δ-aminolaevulinate synthetase from cultured soybean cells
Autor:Wider de Xifra, E.A.; Stella, A.M.; Del C. Batlle, A.M.
Filiación:Laboratorio de Porfirinas, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, 1428 Nuñez Buenos Aires, Argentina
Año:1978
Volumen:11
Número:2
Página de inicio:93
Página de fin:98
DOI: http://dx.doi.org/10.1016/0304-4211(78)90111-6
Título revista:Plant Science Letters
ISSN:03044211
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044211_v11_n2_p93_WiderdeXifra

Referencias:

  • Mosbach, (1971) Sci. Am., 224, p. 26
  • Goldman, Goldstein, Katchalski, (1971) Biochemical Aspects of Reactions on Solid Supports, p. 1. , G.R. Stark, Academic Press, New York
  • Durand, Baudres, (1975) La Researche, 6, p. 437
  • Wider de Xifra, del C. Batlle, Tigier, δ-aminolaevulinate synthetase in extracts of cultured soybean cells (1971) Biochimica et Biophysica Acta (BBA) - Enzymology, 235, p. 511
  • Miller, Teng, (1967) Proc. 7th Int. Congr. Biochem., p. 1059. , Tokyo
  • Ramaswamy, Madhusudaban, δ-Aminolevulinic acid synthetase from cold-stored potatoes (1973) Biochimica et Biophysica Acta (BBA) - Enzymology, 293, p. 269
  • del C. Batlle, Llambias, Wider de Xifra, Tigier, (1975) Int. J. Biochem., 6, p. 591
  • del C. Batlle, Wider de Xifra, Llambias, (1975) Porphyrin in Human Diseases, p. 131. , M. Doss, S. Karger-Basle
  • Beale, Castlefranco, The Biosynthesis of  -Aminolevulinic Acid in Higher Plants: II. Formation of 14C- -Aminolevulinic Acid from Labeled Precursors in Greening Plant Tissues (1974) PLANT PHYSIOLOGY, 53, p. 297
  • Tait, (1973) Biochem. J., 131, p. 389
  • Stella, Wider de Xifra, del C. Batlle, (1977) Mol. Cell. Biochem., 15. , in press
  • Stella, del C. Batlle, (1977) Int. J. Biochem., 8, p. 353
  • Rossetti, Parera, del C. Batlle, (1977) Acta Phys. Lat., , in press
  • Stella, del C. Batlle, (1978) Plant Sci. Lett., 11, p. 87
  • Tigier, del C. Batlle, Locascio, (1970) Enzymology, 38, p. 43
  • Cuatrecasas, (1967) J. Biol. Chem., 245, p. 3059
  • Wider de Xifra, Mendiara, del C. Batlle, (1972) FEBS Lett., 27, p. 275

Citas:

---------- APA ----------
Wider de Xifra, E.A., Stella, A.M. & Del C. Batlle, A.M. (1978) . Porphyrin biosynthesis-immobilized enzymes and ligands. IX. Studies on δ-aminolaevulinate synthetase from cultured soybean cells. Plant Science Letters, 11(2), 93-98.
http://dx.doi.org/10.1016/0304-4211(78)90111-6
---------- CHICAGO ----------
Wider de Xifra, E.A., Stella, A.M., Del C. Batlle, A.M. "Porphyrin biosynthesis-immobilized enzymes and ligands. IX. Studies on δ-aminolaevulinate synthetase from cultured soybean cells" . Plant Science Letters 11, no. 2 (1978) : 93-98.
http://dx.doi.org/10.1016/0304-4211(78)90111-6
---------- MLA ----------
Wider de Xifra, E.A., Stella, A.M., Del C. Batlle, A.M. "Porphyrin biosynthesis-immobilized enzymes and ligands. IX. Studies on δ-aminolaevulinate synthetase from cultured soybean cells" . Plant Science Letters, vol. 11, no. 2, 1978, pp. 93-98.
http://dx.doi.org/10.1016/0304-4211(78)90111-6
---------- VANCOUVER ----------
Wider de Xifra, E.A., Stella, A.M., Del C. Batlle, A.M. Porphyrin biosynthesis-immobilized enzymes and ligands. IX. Studies on δ-aminolaevulinate synthetase from cultured soybean cells. 1978;11(2):93-98.
http://dx.doi.org/10.1016/0304-4211(78)90111-6