Artículo
Ouahid, Y.; Zaccaro, M.C.; Zulpa, G.; Storni, M.; Stella, A.M.; Bossio, J.C.; Tanuz, M.; Del Campo, F.F. "A single microcystin in a toxic Microcystis bloom from the river Río de la Plata (Argentina)" (2011) International Journal of Environmental Analytical Chemistry. 91(6):525-536
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Abstract:
Microcystis is one of the most common bloom-forming cyanobacteria genera in diverse ecosystems. More than 80% of its strains are toxic, mainly due to their ability to produce metabolites known as microcystins (MC). Here we report on a M. aeruginosa bloom that appeared in the summer of 2001 at a site of the Río de la Plata, within the city of Buenos Aires. The symptoms in mice indicated that the bloom was hepatotoxic. LC-PDA analysis revealed a similar high concentration (0.9-1 mg g-1 d w) of just one MC in the bloom biomass during the 3-month study period. During this period most of the MC (ca. 98 %) was found intracellularly, and the content remained almost the same. The molecular mass of the bloom MC was 1036 Da, as deduced from LC-ESI-MS data. Fragment ion analysis by LC-IT-MS-MS allowed identifying 6 out of the 7 amino acids, as well as their position in the molecule. The molecular mass of the unidentified amino acid residue was 155 Da. According to the data obtained, the MC under study was MC-XR, X representing the unidentified amino acid. This is the first report both on the characterisation of MCs in an urban site of the Río de la Plata waters, and on an Argentinean bloom exhibiting only one MC variant. © 2011 Taylor & Francis.
Registro:
| Documento: | Artículo |
| Título: | A single microcystin in a toxic Microcystis bloom from the river Río de la Plata (Argentina) |
| Autor: | Ouahid, Y.; Zaccaro, M.C.; Zulpa, G.; Storni, M.; Stella, A.M.; Bossio, J.C.; Tanuz, M.; Del Campo, F.F. |
| Filiación: | Departamento de Biología, Universidad Autónoma de Madrid, Darwin 2, 28049-Madrid, Spain Laboratorio de Fisiología Vegetal, Biología de Cyanobacteria, Facultad de Ciencias Exactas Naturales, Intendente Güiraldes 2620, C1428EHA Buenos Aires, Argentina Laboratorio de Ecoporfirinas, Facultad de Ciencias Exactas Naturales, Intendente Güiraldes 2620m, C1428EHA Buenos Aires, Argentina Prefectura Naval Argentina, Avenida Eduardo Madero 235, C1000WBK Buenos Aires, Argentina |
| Palabras clave: | Cyanobacteria; Cyanotoxicity; HPLC-PDA; LC-IT-MS/MS; Microcystin; Microcystis aeruginosa; Cyanobacteria; Cyanotoxicity; HPLC-PDA; LC-IT-MS/MS; Microcystin; Microcystis aeruginosa; Amino acids; Molecular mass; Toxic materials; Cyanobacteria; Microcystis; Microcystis aeruginosa; Mus |
| Año: | 2011 |
| Volumen: | 91 |
| Número: | 6 |
| Página de inicio: | 525 |
| Página de fin: | 536 |
| DOI: | http://dx.doi.org/10.1080/03067310903359492 |
| Título revista: | International Journal of Environmental Analytical Chemistry |
| Título revista abreviado: | Int. J. Environ. Anal. Chem. |
| ISSN: | 03067319 |
| CODEN: | IJEAC |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03067319_v91_n6_p525_Ouahid |
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Citas:
---------- APA ----------
Ouahid, Y., Zaccaro, M.C., Zulpa, G., Storni, M., Stella, A.M., Bossio, J.C., Tanuz, M.,..., Del Campo, F.F. (2011) . A single microcystin in a toxic Microcystis bloom from the river Río de la Plata (Argentina). International Journal of Environmental Analytical Chemistry, 91(6), 525-536.http://dx.doi.org/10.1080/03067310903359492
---------- CHICAGO ----------
Ouahid, Y., Zaccaro, M.C., Zulpa, G., Storni, M., Stella, A.M., Bossio, J.C., et al. "A single microcystin in a toxic Microcystis bloom from the river Río de la Plata (Argentina)" . International Journal of Environmental Analytical Chemistry 91, no. 6 (2011) : 525-536.http://dx.doi.org/10.1080/03067310903359492
---------- MLA ----------
Ouahid, Y., Zaccaro, M.C., Zulpa, G., Storni, M., Stella, A.M., Bossio, J.C., et al. "A single microcystin in a toxic Microcystis bloom from the river Río de la Plata (Argentina)" . International Journal of Environmental Analytical Chemistry, vol. 91, no. 6, 2011, pp. 525-536.http://dx.doi.org/10.1080/03067310903359492
---------- VANCOUVER ----------
Ouahid, Y., Zaccaro, M.C., Zulpa, G., Storni, M., Stella, A.M., Bossio, J.C., et al. A single microcystin in a toxic Microcystis bloom from the river Río de la Plata (Argentina). Int. J. Environ. Anal. Chem. 2011;91(6):525-536.http://dx.doi.org/10.1080/03067310903359492
