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Abstract:

The aim of this study was to investigate the phenotypic and genotypic characteristics of Streptococcus uberis isolated from subclinical mastitis (SCM) cases, and to examine the possible association between both characteristics. A total of 32 S. uberis were isolated from 772 quarter milk samples (SCM > 250,000 cells/ml) collected from 195 cows selected randomly from 18 dairy farms located in Argentina. The S. uberis strains were characterized phenotypically by the presence of virulence factors as plasminogen activator factor (PAF), hyaluronidase (HYA), capsule (CAP) and CAMP factor, and were further characterized genotypically by pulsed-field gel electrophoresis (PFGE). S. uberis strains expressed plasminogen activator factor, hyaluronidase or capsule (65.5 %, 56.3 %, 59.4 %, respectively), but only 25 % of isolates were CAMP factor positive. Thirteen different virulence profiles were identified on the basis of the combination of virulence factors. Eighteen PFGE patterns with 90% of similarity were identified among 32 S. uberis. A great diversity of virulence profiles and PFGE patterns were present among dairy farms. S. uberis strains with the same PFGE pattern showed different virulence profiles. Bovine S. uberis strains causing SCM included in the present study showed heterogeneity in regard to their phenotypic and genotypic characteristics, and the PFGE patterns are not associated with the virulence profiles.

Registro:

Documento: Artículo
Título:Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in Argentinean dairy farms
Autor:Lasagno, M.C.; Reinoso, E.B.; Dieser, S.A.; Calvinho, L.F.; Buzzola, F.; Vissio, C.; Bogni, C.I.; Odierno, L.M.
Filiación:Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, (5804) Río Cuarto, Córdoba, Argentina
Departamento de Patología Animal, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, (5804) Río Cuarto, Córdoba, Argentina
Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria, (2300) Rafaela, Santa Fe, Argentina
Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, (1121) Buenos Aires, Argentina
Idioma: Inglés
Palabras clave:Bovine subclinical mastitis; Pulsed-field gel electrophoresis; Streptococcus uberis; Virulence factors; cyclic AMP; hyaluronidase; plasminogen activator; Argentina; article; bacterial membrane; bacterial virulence; bacterium isolation; bovine mastitis; dairying; genetic heterogeneity; genotype; milk; nonhuman; phenotype; Streptococcus uberis; Animal Husbandry; Animals; Argentina; Asymptomatic Infections; Bacterial Capsules; Bacterial Proteins; Bacterial Typing Techniques; Cattle; Dairying; DNA, Bacterial; Electrophoresis, Gel, Pulsed-Field; Female; Genotype; Hemolysin Proteins; Hyaluronoglucosaminidase; Mastitis, Bovine; Phenotype; Streptococcal Infections; Streptococcus; Virulence
Año:2011
Volumen:43
Número:3
Página de inicio:212
Página de fin:217
Título revista:Revista Argentina de Microbiologia
Título revista abreviado:Rev. Argent. Microbiol.
ISSN:03257541
CODEN:RAMID
CAS:cyclic AMP, 60-92-4; hyaluronidase, 488712-31-8, 9001-54-1, 9055-18-9; plasminogen activator, 9039-53-6; Bacterial Capsules; Bacterial Proteins; CAMP protein, Streptococcus; DNA, Bacterial; Hemolysin Proteins; Hyaluronoglucosaminidase, 3.2.1.35
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Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v43_n3_p212_Lasagno

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Citas:

---------- APA ----------
Lasagno, M.C., Reinoso, E.B., Dieser, S.A., Calvinho, L.F., Buzzola, F., Vissio, C., Bogni, C.I.,..., Odierno, L.M. (2011) . Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in Argentinean dairy farms . Revista Argentina de Microbiologia, 43(3), 212-217.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v43_n3_p212_Lasagno [ ]
---------- CHICAGO ----------
Lasagno, M.C., Reinoso, E.B., Dieser, S.A., Calvinho, L.F., Buzzola, F., Vissio, C., et al. "Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in Argentinean dairy farms " . Revista Argentina de Microbiologia 43, no. 3 (2011) : 212-217.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v43_n3_p212_Lasagno [ ]
---------- MLA ----------
Lasagno, M.C., Reinoso, E.B., Dieser, S.A., Calvinho, L.F., Buzzola, F., Vissio, C., et al. "Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in Argentinean dairy farms " . Revista Argentina de Microbiologia, vol. 43, no. 3, 2011, pp. 212-217.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v43_n3_p212_Lasagno [ ]
---------- VANCOUVER ----------
Lasagno, M.C., Reinoso, E.B., Dieser, S.A., Calvinho, L.F., Buzzola, F., Vissio, C., et al. Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in Argentinean dairy farms . Rev. Argent. Microbiol. 2011;43(3):212-217.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v43_n3_p212_Lasagno [ ]