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Abstract:

In Bacillus subtilis, osmotolerance is a stationary phase-dependent, adaptive response inhibiting sporulation and sharing common regulators with this process. The extent of this inhibition was determined by measuring transcription activity of promoter lacZ fusions to early sigma genes (spoIIG and spoIIA coding for precursors of oσ(E) and σ(F)) and to reporters of them (spoIID, spoIIQ and spoIIIG), in the absence and presence of 0.6 M or 1 M NaCl. The transcription activity of these sigma precursors, normally occurring at the onset of the stationary phase, was reduced to 30-50% of their maximal expression in hyperosmotic conditions; expression of genes under their control was, however, more inhibited (< 10%). Therefore, sporulation was blocked at the sigma σ(E) and σ(F) activation steps. This assumption was confirmed by electron microscopic examinations of hyperosmotic cultures, which presented asymmetric septa characteristic of stage II mutants. Discussion was focused on the particular composition and/or structure of membranes during hyperosmotic growth and their involvement in the arrest of sporulation.

Registro:

Documento: Artículo
Título:Osmotic strength blocks sporulation at stage II by impeding activation of early sigma factors in Bacillus subtilis
Autor:Ruzal, S.M.; López, C.; Rivas, E.; Sánchez-Rivas, C.
Filiación:Fac. de Cie. Exactas y Naturales, Depto. de Quim. Biológica, Ciudad Universitaria, Nuñez, 1428 Buenos Aires, Argentina
Facultad de Medicina, Inst. de Biol. Cel. y Neurociencias, Paraguay 2155 2piso, Capital Federal, 1121 Buenos Aires, Argentina
Palabras clave:beta galactosidase; sigma factor; sodium chloride; article; bacillus subtilis; controlled study; electron microscopy; gene expression; genetic transcription; hyperosmolarity; nonhuman; osmoregulation; priority journal; promoter region; sporogenesis; Bacillus subtilis; Bacterial Proteins; Gene Expression; Gene Expression Regulation; Genes, Bacterial; Microscopy, Electron; Osmolar Concentration; Sigma Factor; Sodium Chloride; Spores; Spores, Bacterial; Time Factors; Transcription Factors; Bacillus subtilis; Posibacteria; Prokaryota
Año:1998
Volumen:36
Número:2
Página de inicio:75
Página de fin:79
DOI: http://dx.doi.org/10.1007/s002849900282
Título revista:Current Microbiology
Título revista abreviado:Curr. Microbiol.
ISSN:03438651
CODEN:CUMID
CAS:Bacterial Proteins; Sigma Factor; Sodium Chloride, 7647-14-5; spoIIR protein, Bacillus subtilis; spore-specific proteins, Bacillus; Transcription Factors
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v36_n2_p75_Ruzal

Referencias:

  • Bohin, J.P., Rigomier, D., Sohaeffer, P., Ethanol sensitivity of sporulation in Bacillus subtilis: A new tool for the analysis of the sporulation process (1976) J Bacteriol, 127, pp. 934-940
  • Deuerling, E., Paeslack, B., Schumann, W., The ftsH gene of Bacillus subtilis is transintly induced after osmotic temperature upshift (1995) J Bacteriol, 177, pp. 4105-4112
  • Deuerling, E., Mogk, A., Richter, C., Purucker, M., Schumann, W., The ftsH gene of Bacillus subtilis is involved in major cellular processes such as sporulation, stress adaptation and secretion (1997) Mol Microbiol, 23, pp. 921-933
  • Duncan, L., Alper, S., Arigoni, F., Losick, R., Stragier, P., Activation of cell-specific transcription by a serine phosphatase at the site of asymmetric division (1995) Science, 270, pp. 641-644
  • Errington, J., Bacillus subtilis sporulation: Regulation of gene expression and control of morphogenesis (1993) Microbiol Rev, 57, pp. 1-33
  • Harry, E.J., Pogliano, K., Losick, R., Use of immunofluorescence to visualize cell-specific gene expression during sporulation in Bacillus subtilis (1995) J Bacteriol, 177, pp. 3386-3393
  • Higgins, M.A., Piggot, P.J., Septal membrane fusion: A pivotal event in bacterial spore formation? (1992) Mol Microbiol, 6, pp. 2565-2571
  • Ireton, K., Grossman, A.D., Coupling between gene expression and DNA synthesis early during development in Bacillus subtilis (1992) Proc Natl Acad Sci USA, 89, pp. 8808-8812
  • Lopez, C., Heras, H., Ruzal, S.M., Sanchez-Rivas, C., Rivas, E., Variations of the envelope composition of Bacillus subtilis during growth in hyperosmotic medium (1997) Current Microbiol, , in press
  • Losick, R., Stragier, P., Crisscross regulation of cell-typespecific gene expression during development in Bacillus subtilis (1992) Nature, 355, pp. 601-604
  • Mason, J.M., Hackett, R.H., Setlow, P., Studies on the regulation of expression of genes coding for small, acid-soluble proteins of Bacillus subtilis spores using lacZ gene fusions (1988) J Bacteriol, 170, pp. 239-244
  • Miller, J.H., (1972) Experiments in Molecular Genetics, , Cold Spring Harbor, NY: Cold Spring Harbor Laboratory
  • Oh, Y.K., Freese, E.B., Freese, E., Abnormal septation and inhibition of sporulation by accumulation of L-α glycerophosphate in Bacillus subtilis mutants (1973) J Bacteriol, 113, pp. 1034-1045
  • Paradies, G., Petrosillo, G., Ruggiero, F.M., Cardiolipin-dependent decrease of cytochrome c oxidase activity in heart mitochondria from hypothyroid rats (1997) Biochim Biophys Acta, 1319, pp. 5-8
  • Ruzal, S.M., Sanchez-Rivas, C., Physiological and genetic characterization of the osmotic stress response in Bacillus subtilis (1994) Can J Microbiol, 40, pp. 140-144
  • Ruzal, S.M., Sanchez-Rivas, C., (1997) SpoOA and DegU, Two Alternative Gene Functions for Sporulation and Osmotolerance in Bacillus Subtilis, , Submitted
  • Schaeffer, P., Millet, J., Aubert, J., Catabolic repression of bacterial sporulation (1965) Proc Natl Acad Sci USA, 54, pp. 701-711
  • Shazand, K., Frandsen, N., Stragier, P., Cell-type specificity during development in Bacillus subtilis: The molecular and morphological requirements for sigma E activation (1995) EMBO J, 14, pp. 1439-1445
  • Skorko-Glonek, J., Lipinska, B., Krzewski, K., Zolese, G., Bertoli, E., Tanfani, F., HtrA heat shock protease interacts with phospholipid membranes and undergoes conformational changes (1997) J Biol Chem, 272, pp. 8974-8982
  • Stragier, P., Losick, R., Cascades of sigma factors revisited (1990) Mol Microbiol, 4, pp. 1801-1806
  • Strauch, M.A., De Mendoza, D., Hoch, J.A., cis-unsaturated fatty acids specifically inhibit a signal-transducing protein kinase required for initiation of sporulation in Bacillus subtilis (1992) Mol Microbiol, 6, pp. 2909-2917
  • Trach, K.A., Hoch, J.A., Multisensory activation of the phosphorelay initiating sporulation in Bacillus subtilis: Identification and sequence of the protein kinase of the alternate pathway (1993) Mol Microbiol, 8, pp. 69-79
  • Waites, W.M., Kay, D., Dawes, I.W., Wood, D.A., Warren, S.C., Mandelstam, J., Sporulation in Bacillus subtilis. Correlation of biochemical events with morphological changes in asporogenous mutants (1970) Bioehem J, 118, pp. 667-676
  • Zhang, L., Higgins, M.L., Piggot, P.J., Karow, M.L., Analysis of the role of prespore gene expression in the compartmentalization of mother cell-specific gene expression during sporulation of Bacillus subtilis (1996) J Bacteriol, 178, pp. 2813-2817

Citas:

---------- APA ----------
Ruzal, S.M., López, C., Rivas, E. & Sánchez-Rivas, C. (1998) . Osmotic strength blocks sporulation at stage II by impeding activation of early sigma factors in Bacillus subtilis. Current Microbiology, 36(2), 75-79.
http://dx.doi.org/10.1007/s002849900282
---------- CHICAGO ----------
Ruzal, S.M., López, C., Rivas, E., Sánchez-Rivas, C. "Osmotic strength blocks sporulation at stage II by impeding activation of early sigma factors in Bacillus subtilis" . Current Microbiology 36, no. 2 (1998) : 75-79.
http://dx.doi.org/10.1007/s002849900282
---------- MLA ----------
Ruzal, S.M., López, C., Rivas, E., Sánchez-Rivas, C. "Osmotic strength blocks sporulation at stage II by impeding activation of early sigma factors in Bacillus subtilis" . Current Microbiology, vol. 36, no. 2, 1998, pp. 75-79.
http://dx.doi.org/10.1007/s002849900282
---------- VANCOUVER ----------
Ruzal, S.M., López, C., Rivas, E., Sánchez-Rivas, C. Osmotic strength blocks sporulation at stage II by impeding activation of early sigma factors in Bacillus subtilis. Curr. Microbiol. 1998;36(2):75-79.
http://dx.doi.org/10.1007/s002849900282