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Abstract:

The transformation efficiency of competent Bacillus subtilis degU32(Hy) strains was found to depend on the marker that was selected. Protrophic transformants were obtained at frequencies similar to those in the wild type control, but Spo - transformants oere rare also when a spoOA::erm insertion that produces a selectable marker (Erm R ) was used. The Erm R transformants obtained within the degU32(Hy) background were Spo + and had lost the characteristics of the DegU(Hy) parental recipient strain i.e., secretion of exo-enzymes and sporulation resistance to catabolites. The spoOA::erm insertion was mapped to a location near degU. The similarities between the spoOA and degU sequences and the metabolic interferences between the mutated products which result in this unexpected recombination, are discussed. © 1993.

Registro:

Documento: Artículo
Título:The spoOA and degU genes of Bacillus subtilis show genetic homology
Autor:Cucchi, A.; Sanchez-Rivas, C.
Filiación:Departamento de Química Biológica, Facultad de Ciencías Exactas y Naturales, Ciudad Universitaria, Buenos Aires, Argentina
Palabras clave:Bacillus subtilis; Exo-enzymes; spoOA, degU; Sporulation control genes; Transformation efficiency; article; bacillus subtilis; genetic transformation; nonhuman; priority journal; sequence homology; sporogenesis; Bacillus subtilis; Chromosome Mapping; DNA Transposable Elements; Genes, Bacterial; Genetic Markers; Mutation; Spores, Bacterial; Support, Non-U.S. Gov't; Transduction, Genetic; Transformation, Genetic
Año:1993
Volumen:107
Número:2-3
Página de inicio:307
Página de fin:312
DOI: http://dx.doi.org/10.1111/j.1574-6968.1993.tb06048.x
Título revista:FEMS Microbiology Letters
Título revista abreviado:FEMS Microbiol. Lett.
ISSN:03781097
CODEN:FMLED
CAS:DNA Transposable Elements; Genetic Markers
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v107_n2-3_p307_Cucchi

Referencias:

  • Ferrari, Trach, Le Coq, Spence, Ferrari, Hoch, (1985) Proc. Natl. Acad. Sci. USA, 82, pp. 2647-2651. , 2nd Edn
  • Kunst, Pascal, Lepesant-Kejzlarova, Lepesant, Billaut, Dedonder, (1974) Biochimie, 56, pp. 1481-1489
  • Dedonder, Lepesant, Lepesant-Kejzlarova, Billaut, Steinmetz, Kunst, (1977) Appl. Env. Microbiol., 33, pp. 989-993
  • Lévi, Sanchez-Rivas, Schaeffer, (1977) FEMS Microbiol. Lett., 2, pp. 323-326
  • Le Dérout, Thaler, Guillén, Hirschbein, (1992) Mol. Microbiol., 6, pp. 1495-1505
  • Henner, Yang, Ferrari, (1988) J. Bacteriol., 170, pp. 5102-5109
  • Miller, J.H. (1972) Cold Spring Harbor, NY; Schaeffer, Ionesco, Ryter, Balassa, (1963) Colloq. Int. C.N.R.S., 124, pp. 553-563
  • Spizizen, TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE (1958) Proceedings of the National Academy of Sciences, 44, pp. 1072-1078. , 2nd Edn
  • Anagnostopoulos, Spizizen, (1961) J. Bacteriol., 81, pp. 1-8
  • Niaudet, Erlich, (1979) Plasmid, 2, pp. 48-58
  • Contente, Dubnau, (1979) Mol. Gen. Genet., 176, pp. 251-258
  • Saito, Miurak, (1963) Biochem. Biophys. Acta, 72, pp. 619-629
  • Chang, Cohen, (1979) Mol. Gen. Genet., 168, pp. 111-115
  • Rubinstein, Moratinos, Coso, Sanchez-Rivas, (1988) FEMS Microbiol. Lett., 56, pp. 67-70
  • Birnboim, Doly, (1979) Nucleic Acids Res., 7, pp. 1513-1523
  • Maniatis, Fritsch, Sambrook, (1982) Molecular Cloning. A Laboratory Manual, , 2nd Edn., Cold Spring Harbor, NY
  • Takahashi, (1963) J. Gen. Microbiol., 31, pp. 211-217
  • Msadek, Kunst, Henner, Klier, Rapoport, Dedonder, (1990) J. Bacteriol., 172, pp. 824-834
  • Tanaka, Kawata, (1988) J. Bacteriol., 170, pp. 3593-3600
  • Roggiani, Hahn, Dubnau, (1990) J. Bacteriol., 172, pp. 4056-4063
  • Kunst, Debarbouillé, Msadek, Young, Mauel, Karamata, Klier, Dedonder, (1988) J. Bacteriol., 170, pp. 5093-5101
  • Perego, Spiegelman, Hoch, (1988) Mol. Microbiol., 2, pp. 689-699
  • Strauch, Webb, Spiegelman, Hoch, (1990) Proc. Natl. Acad. Sci. USA, 87, pp. 1801-1805. , 2nd Edn
  • Burbulys, Trach, Hoch, (1991) Cell, 64, pp. 545-552
  • Antoniewski, Savelli, Stragier, (1990) J. Bacteriol., 172, pp. 86-93
  • Hoch, Trach, Kawamura, Saito, (1985) J. Bacteriol., 161, pp. 552-555
  • Schaeffer, Millet, Aubert, (1965) Proc. Natl. Acad. Sci. USA, 54, pp. 704-711. , 2nd Edn
  • Spiegelman, Van Hoy, Perego, Day, Track, Hoch, (1990) J. Bacteriol., 172, pp. 5011-5019

Citas:

---------- APA ----------
Cucchi, A. & Sanchez-Rivas, C. (1993) . The spoOA and degU genes of Bacillus subtilis show genetic homology. FEMS Microbiology Letters, 107(2-3), 307-312.
http://dx.doi.org/10.1111/j.1574-6968.1993.tb06048.x
---------- CHICAGO ----------
Cucchi, A., Sanchez-Rivas, C. "The spoOA and degU genes of Bacillus subtilis show genetic homology" . FEMS Microbiology Letters 107, no. 2-3 (1993) : 307-312.
http://dx.doi.org/10.1111/j.1574-6968.1993.tb06048.x
---------- MLA ----------
Cucchi, A., Sanchez-Rivas, C. "The spoOA and degU genes of Bacillus subtilis show genetic homology" . FEMS Microbiology Letters, vol. 107, no. 2-3, 1993, pp. 307-312.
http://dx.doi.org/10.1111/j.1574-6968.1993.tb06048.x
---------- VANCOUVER ----------
Cucchi, A., Sanchez-Rivas, C. The spoOA and degU genes of Bacillus subtilis show genetic homology. FEMS Microbiol. Lett. 1993;107(2-3):307-312.
http://dx.doi.org/10.1111/j.1574-6968.1993.tb06048.x