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Abstract:

Tyrosine aminotransferase purified from epimastigotes of Trypanosoma cruzi displays an additional activity of alanine aminotransferase, absent in all other tyrosine aminotransferases characterized so far. Since the parasite's genome contains a high number of copies of the tyrosine aminotransferase gene, we could not rule out the possibility that two very similar proteins, with changed specificity due to a few amino acid substitutions, might be responsible for the two activities. We have now expressed in Escherichia coli a recombinant tyrosine aminotransferase as a fusion protein with glutathione S-trans-ferase. The purified fusion protein, intact or after thrombin cleavage, displays tyrosine aminotransferase and alanine aminotransferase activities with apparent Km values similar to those for the natural enzyme, thus proving that they belong to the same protein. © 1995.

Registro:

Documento: Artículo
Título:A recombinant tyrosine aminotransferase from Trypanosoma cruzi has both tyrosine aminotransferase and alanine aminotransferase activities
Autor:Montemartini, M.; Búa, J.; Bontempi, E.; Zelada, C.; Ruiz, a.M.; Santomé, JosA.; JoséCazzulo, J.; Nowicki, C.
Filiación:IQUIFIB (UBA-CON/CET), Facultad de Farmacia v Bioquímica, Universidad de Buenos Aires, Junin 956, 1113 Buenos Aires, Argentina
Instituto National de Diagnóstico e Investigatión de la Enfermedad de Chagas 'Dr. Mario Fatala Chaben', Ministerio de Salud y Acción Social, Ar. Paseo Colon 563, 1063 Buenos Aires, Argentina
Instituto de Investigations Bioquímicas 'Luis F. Leloir', Fundatión Camponar, Facultad de Ciencias Exactas y Naturales, 1405 Buenos Aires, Argentina
Palabras clave:Alanine aminotransferase; Fusion protein; Recombinant transaminase; Trypanosoma cruzi; Tyrosine aminotransferase; alanine aminotransferase; hybrid protein; tyrosine aminotransferase; article; controlled study; enzyme activity; molecular cloning; nonhuman; priority journal; trypanosoma cruzi; Alanine Transaminase; Amino Acid Sequence; Animal; Blotting, Western; Escherichia coli; Gene Expression Regulation, Enzymologic; Glutathione Transferase; Molecular Sequence Data; Recombinant Proteins; Support, Non-U.S. Gov't; Trypanosoma cruzi; Tyrosine Transaminase
Año:1995
Volumen:133
Número:1-2
Página de inicio:17
Página de fin:20
DOI: http://dx.doi.org/10.1016/0378-1097(95)00323-W
Título revista:FEMS Microbiology Letters
Título revista abreviado:FEMS Microbiol. Lett.
ISSN:03781097
CODEN:FMLED
CAS:alanine aminotransferase, 9000-86-6, 9014-30-6; tyrosine aminotransferase, 9014-55-5; Alanine Transaminase, EC 2.6.1.2; Glutathione Transferase, EC 2.5.1.18; Recombinant Proteins; Tyrosine Transaminase, EC 2.6.1.5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v133_n1-2_p17_Montemartini

Referencias:

  • Montemartini, Santomé, Cazzulo, Nowicki, Purification and partial structural and kinetic characterization of tyrosine aminotransferase from epimastigotes of Trypanosoma cruzi (1993) Biochem. J., 292, pp. 901-906
  • Bontempi, Búa, Åslund, Porcel, Segura, Henriksson, Orn, Ruiz, Isolation and characterization of a gene from Trypanosoma cruzi encoding a 46-kilodalton protein with homology to human and rat tyrosine aminotransferases (1993) Mol. Biochem. Parasitol., 59, pp. 253-262
  • Guan, Dixon, Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase (1991) Anal. Biochem., 192, pp. 262-267
  • Berthold, Frorath, Scanarini, Abney, Ernst, Northemann, An alternative system for expression and purification of recombinant proteins (1992) Biotechnol. Lett., 14, pp. 245-250
  • Smith, Johnson, Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase (1988) Gene, 647, pp. 31-40
  • Montemartini, Santomé, Cazzulo, Nowicki, Purification and partial structural and kinetic characterization of an aromatic L-α-hydroxy acid dehydrogenase from epimastigotes of Trypanosoma cruzi (1994) Molecular and Biochemical Parasitology, 68, pp. 15-23
  • Gelehrter, Emanuel, Spencer, Induction of tyrosine aminotransferase by dexamethasone, insulin, and serum. Characterization of the induced enzyme (1972) J. Biol. Chem., 247, pp. 6197-6203
  • Fraser, Suzuki, The use of least squares in the data analysis (1973) Physical Principles and Techniques of Protein Chemistry, 21, pp. 301-355. , 2nd edn., S.J. Leach, Academic Press, New York, NY, Part C

Citas:

---------- APA ----------
Montemartini, M., Búa, J., Bontempi, E., Zelada, C., Ruiz, a.M., Santomé, JosA., JoséCazzulo, J.,..., Nowicki, C. (1995) . A recombinant tyrosine aminotransferase from Trypanosoma cruzi has both tyrosine aminotransferase and alanine aminotransferase activities. FEMS Microbiology Letters, 133(1-2), 17-20.
http://dx.doi.org/10.1016/0378-1097(95)00323-W
---------- CHICAGO ----------
Montemartini, M., Búa, J., Bontempi, E., Zelada, C., Ruiz, a.M., Santomé, JosA., et al. "A recombinant tyrosine aminotransferase from Trypanosoma cruzi has both tyrosine aminotransferase and alanine aminotransferase activities" . FEMS Microbiology Letters 133, no. 1-2 (1995) : 17-20.
http://dx.doi.org/10.1016/0378-1097(95)00323-W
---------- MLA ----------
Montemartini, M., Búa, J., Bontempi, E., Zelada, C., Ruiz, a.M., Santomé, JosA., et al. "A recombinant tyrosine aminotransferase from Trypanosoma cruzi has both tyrosine aminotransferase and alanine aminotransferase activities" . FEMS Microbiology Letters, vol. 133, no. 1-2, 1995, pp. 17-20.
http://dx.doi.org/10.1016/0378-1097(95)00323-W
---------- VANCOUVER ----------
Montemartini, M., Búa, J., Bontempi, E., Zelada, C., Ruiz, a.M., Santomé, JosA., et al. A recombinant tyrosine aminotransferase from Trypanosoma cruzi has both tyrosine aminotransferase and alanine aminotransferase activities. FEMS Microbiol. Lett. 1995;133(1-2):17-20.
http://dx.doi.org/10.1016/0378-1097(95)00323-W