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Abstract:

Conjugal transfer of lactose-fermenting ability (Lac+) and lytic phage resistance (Lpr+) was demonstrated in four matings between Lactococcus lactis ssp. lactis C2, phage resistant mutant donor strains C21, C23 and C26 and the C2 derivative LM2302 plasmid free recipient strain. Transconjugants were selected with Lac+ Emr and/or Str phenotypes. Lac+ was transferred from donor strains to Lac- Emr and r Str phage sensitive recipient strains at a frequency of 10-6 to 10-8 revealing that Lac+ and subsequently Lpr+ were transferred. Although clumping (Clu) effects with a subsequent high frequency transconjugation (Hft) have been observed before in related strains, it was not evident in this case. Phage assays with Argentine (ARG) and United States (US) phage revealed that recipient strains were Lpr-. Because the transconjugants had the same lytic pattern as the corresponding donor strain, it is apparent that the unselected Lpr+ trait was cotransferred with the Lac+ marker on a 45 kb plasmid. A significant reduction in bacteriophage adsorption and failures in plaque formations for the ARG and the US prolate phages in all Lpr+ strains was also observed. These results suggest that the Lpr+ trait is mobilized by conjugal transfer. © 1992 Academic Press Limited.

Registro:

Documento: Artículo
Título:Conjugal transfer of bacteriophage resistance to prolate phages in Lactococcus lactis
Autor:De Fabrizio, S.V.; Ledford, R.A.; Parada, J.L.; Pan, C.L.
Filiación:Laboratorio de Microbiologia de Alimentos, Facultad de Ciencias Exactas y Naturales-UBA, Buenos Aires, Argentina
Institute of Food Science, Cornell University, Ithaca, NY 14853, United States
Año:1992
Volumen:9
Número:3
Página de inicio:251
Página de fin:263
DOI: http://dx.doi.org/10.1016/0740-0020(92)80053-7
Título revista:Food Microbiology
Título revista abreviado:Food Microbiol.
ISSN:07400020
CODEN:FOMIE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07400020_v9_n3_p251_DeFabrizio

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Citas:

---------- APA ----------
De Fabrizio, S.V., Ledford, R.A., Parada, J.L. & Pan, C.L. (1992) . Conjugal transfer of bacteriophage resistance to prolate phages in Lactococcus lactis. Food Microbiology, 9(3), 251-263.
http://dx.doi.org/10.1016/0740-0020(92)80053-7
---------- CHICAGO ----------
De Fabrizio, S.V., Ledford, R.A., Parada, J.L., Pan, C.L. "Conjugal transfer of bacteriophage resistance to prolate phages in Lactococcus lactis" . Food Microbiology 9, no. 3 (1992) : 251-263.
http://dx.doi.org/10.1016/0740-0020(92)80053-7
---------- MLA ----------
De Fabrizio, S.V., Ledford, R.A., Parada, J.L., Pan, C.L. "Conjugal transfer of bacteriophage resistance to prolate phages in Lactococcus lactis" . Food Microbiology, vol. 9, no. 3, 1992, pp. 251-263.
http://dx.doi.org/10.1016/0740-0020(92)80053-7
---------- VANCOUVER ----------
De Fabrizio, S.V., Ledford, R.A., Parada, J.L., Pan, C.L. Conjugal transfer of bacteriophage resistance to prolate phages in Lactococcus lactis. Food Microbiol. 1992;9(3):251-263.
http://dx.doi.org/10.1016/0740-0020(92)80053-7