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Abstract:

The pseudofilamentous red alga Chroodactylon ornatum was grown in f/2 culture medium with the addition of 10, 34, and 100 μM (nominal concentration) cupric sulfate. The bioassays were terminated at two selected end points (days 4 and 12). Growth inhibition, changes in pigment composition, and oxidative stress indicators such as phenolic compounds and lipid peroxidation (dosed as thiobarbituric reactive substances) were observed in cultures with 34 and 100-μM cupric sulfate. Quinacrine (Atebrin) and chlorotetracycline fluorochromes showed abundant vacuoles of acidic content, related with mucilage secretion. Structural analyses by methylation, desulfation-methylation, alkaline treatment, and NMR spectroscopy revealed that the mucilaginous sheath of C. ornatum contains a sulfated galactan with a backbone of alternating 3-linked β-d-galactopyranose and 4-linked α-l-galactopyranose moieties, i.e., an agaran. The absence of 3,6-anhydrogalactose and of its precursor unit (α-galactose 6-sulfate) were confirmed. The highly sulfated polysaccharide contained these ester groups on the C-2 and C-4 hydroxyl groups of the 3-linked unit and on C-3 of the 4-linked units. The large proportion of sulfate esterification in this polysaccharide can be related to the extracellular biosorption of copper divalent cation (2.5 ± 0.4 mg per gram of dry weight) and to copper tolerance in bioassays. © 2014 Springer Science+Business Media Dordrecht.

Registro:

Documento: Artículo
Título:A sulfated galactan from the mucilaginous sheath of the red filamentous alga Chroodactylon ornatum (Stylonematophyceae, Rhodophyta)
Autor:Cabrera, J.M.; Stortz, C.A.; Rodríguez, M.C.
Filiación:Departamento de Química Orgánica-CIHIDECAR, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Departamento de Biodiversidad y Biología Experimental, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina
INIBIOMA - CRUB, Universidad del Comahue, 8400 Bariloche, Argentina
Palabras clave:Biosorption; Chroodactylon ornatum; Copper bioassays; Mucilage; Sulfated galactan; algae; Chroodactylon ornatum; Rhodophyta; Stylonematophyceae
Año:2014
Volumen:26
Número:4
Página de inicio:1801
Página de fin:1811
DOI: http://dx.doi.org/10.1007/s10811-014-0236-3
Título revista:Journal of Applied Phycology
Título revista abreviado:J. Appl. Phycol.
ISSN:09218971
CODEN:JAPPE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09218971_v26_n4_p1801_Cabrera

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Citas:

---------- APA ----------
Cabrera, J.M., Stortz, C.A. & Rodríguez, M.C. (2014) . A sulfated galactan from the mucilaginous sheath of the red filamentous alga Chroodactylon ornatum (Stylonematophyceae, Rhodophyta). Journal of Applied Phycology, 26(4), 1801-1811.
http://dx.doi.org/10.1007/s10811-014-0236-3
---------- CHICAGO ----------
Cabrera, J.M., Stortz, C.A., Rodríguez, M.C. "A sulfated galactan from the mucilaginous sheath of the red filamentous alga Chroodactylon ornatum (Stylonematophyceae, Rhodophyta)" . Journal of Applied Phycology 26, no. 4 (2014) : 1801-1811.
http://dx.doi.org/10.1007/s10811-014-0236-3
---------- MLA ----------
Cabrera, J.M., Stortz, C.A., Rodríguez, M.C. "A sulfated galactan from the mucilaginous sheath of the red filamentous alga Chroodactylon ornatum (Stylonematophyceae, Rhodophyta)" . Journal of Applied Phycology, vol. 26, no. 4, 2014, pp. 1801-1811.
http://dx.doi.org/10.1007/s10811-014-0236-3
---------- VANCOUVER ----------
Cabrera, J.M., Stortz, C.A., Rodríguez, M.C. A sulfated galactan from the mucilaginous sheath of the red filamentous alga Chroodactylon ornatum (Stylonematophyceae, Rhodophyta). J. Appl. Phycol. 2014;26(4):1801-1811.
http://dx.doi.org/10.1007/s10811-014-0236-3