Artículo

Aznar, C.; Liegeard, P.; Mariette, C.; Lafon, S.; Levin, M.J.; Hontebeyrie, M. "A simple Trypanosoma cruzi enzyme-linked immunoassay for control of human infection in nonendemic areas" (1997) FEMS Immunology and Medical Microbiology. 18(1):31-37
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Abstract:

An enzyme linked immunosorbent assay (ELISA) was developed for detecting IgM and IgG antibodies against Trypanosoma cruzi in blood hank donors from endemic or nonendemic areas. A crude extract of trypomastigotes from cultures was used as antigen. A total of 494 serum samples from patients with acute, congenital, or chronic form of Chagas' disease, and from healthy French individuals were studied. The sensitivity of the ELISA was determined with 89 serum samples from chagasic patients and was evaluated to 98.8%. The specificity was determined with 405 serum samples from French blood transfusion centers donors and evaluated to 98.3%. Two hundred and eighty- five serum samples from blood donors from Argentina and Brazil were also tested. Furthermore, in order to assess the absence of cross-reactivity with other protozoan infections, we studied 86 serum samples including (i) 32 individuals with cutaneous leishmaniasis living in a T. cruzi endemic region of Bolivia, and (ii) 54 patients from nonendemic area for Chagas' disease, 19 of them with kala-azar and 35 others with malaria.

Registro:

Documento: Artículo
Título:A simple Trypanosoma cruzi enzyme-linked immunoassay for control of human infection in nonendemic areas
Autor:Aznar, C.; Liegeard, P.; Mariette, C.; Lafon, S.; Levin, M.J.; Hontebeyrie, M.
Filiación:Laboratoire de Parasitologie, Ctr. de Biol. Med. Specialisee, Institut Pasteur, 28 rue du Dr Roux, F-75724 Paris Cedex 15, France
U. de Parasitol. Exp., Institut Pasteur, 28 rue du Dr Roux, F-75724 Paris Cedex 15, France
Lab. Biol. Molec. Enfermedad Chagas, INGEBI-CONICET, 2490, Vuelta de Obligado, 1428, Buenos Aires, Argentina
Palabras clave:Blood bank screening; Chases' disease diagnosis; Sensitivity; Specificity; Transfusion; Trypanosoma cruzi; antigen detection; argentina; article; blood bank; chagas disease; controlled study; diagnostic accuracy; enzyme linked immunosorbent assay; human; infection control; major clinical study; priority journal; screening test; trypanosoma cruzi; trypanosomiasis; trypomastigote; Animals; Antibodies, Protozoan; Blood Donors; Chagas Disease; Endemic Diseases; Enzyme-Linked Immunosorbent Assay; Humans; Immunoglobulin G; Immunoglobulin M; Sensitivity and Specificity; Trypanosoma cruzi
Año:1997
Volumen:18
Número:1
Página de inicio:31
Página de fin:37
DOI: http://dx.doi.org/10.1016/S0928-8244(97)00019-9
Título revista:FEMS Immunology and Medical Microbiology
Título revista abreviado:FEMS IMMUNOL. MED. MICROBIOL.
ISSN:09288244
CODEN:FIMIE
CAS:Antibodies, Protozoan; Immunoglobulin G; Immunoglobulin M
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09288244_v18_n1_p31_Aznar

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Citas:

---------- APA ----------
Aznar, C., Liegeard, P., Mariette, C., Lafon, S., Levin, M.J. & Hontebeyrie, M. (1997) . A simple Trypanosoma cruzi enzyme-linked immunoassay for control of human infection in nonendemic areas. FEMS Immunology and Medical Microbiology, 18(1), 31-37.
http://dx.doi.org/10.1016/S0928-8244(97)00019-9
---------- CHICAGO ----------
Aznar, C., Liegeard, P., Mariette, C., Lafon, S., Levin, M.J., Hontebeyrie, M. "A simple Trypanosoma cruzi enzyme-linked immunoassay for control of human infection in nonendemic areas" . FEMS Immunology and Medical Microbiology 18, no. 1 (1997) : 31-37.
http://dx.doi.org/10.1016/S0928-8244(97)00019-9
---------- MLA ----------
Aznar, C., Liegeard, P., Mariette, C., Lafon, S., Levin, M.J., Hontebeyrie, M. "A simple Trypanosoma cruzi enzyme-linked immunoassay for control of human infection in nonendemic areas" . FEMS Immunology and Medical Microbiology, vol. 18, no. 1, 1997, pp. 31-37.
http://dx.doi.org/10.1016/S0928-8244(97)00019-9
---------- VANCOUVER ----------
Aznar, C., Liegeard, P., Mariette, C., Lafon, S., Levin, M.J., Hontebeyrie, M. A simple Trypanosoma cruzi enzyme-linked immunoassay for control of human infection in nonendemic areas. FEMS IMMUNOL. MED. MICROBIOL. 1997;18(1):31-37.
http://dx.doi.org/10.1016/S0928-8244(97)00019-9