Abstract:
Thermal resistance of freeze-dried α-amylase and α-glucosidase in trehalose matrices (1 to 20 % w/v) stored at 90 °C and relative humidities (RH) between 0 and 44 % was studied. At RH values up to 33 %, 10 % (w/v) trehalose was necessary to retain at least 50 % of α-amylase activity. For α-glucosidase, 10 % (w/v) trehalose was effective only at 0 % RH. Ultrafiltration of the crude enzymatic fermentation extracts enhanced enzyme stability per se. However, ultrafiltration in combination with 1% (w/v) trehalose retained 74 % of α-glucosidase and 95 % of α-amylase activities.
Registro:
Documento: |
Artículo
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Título: | Stabilizing crude and ultrafiltrated α-amylase and α-glucosidase from Aspergillus oryzae by trehalose |
Autor: | Terebiznik, M.R.; Zylberman, V.; Buera, M.P.; Pilosof, A.M.R. |
Filiación: | Dto. de Industrias, FCEyN, C. Universitaria, (1428) Buenos Aires, Argentina Dto. de Ingeniería Química, Fl. Universidad de Buenos Aires, C. Universitaria, (1428) Buenos Aires, Argentina
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Palabras clave: | alpha glucosidase; amylase; enzyme stability; extractive fermentation; freeze drying; heat resistance; humidity; microbe metabolism; trehalose; ultrafiltration; Aspergillus oryzae |
Año: | 1998
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Volumen: | 12
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Número: | 9
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Página de inicio: | 683
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Página de fin: | 687
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DOI: |
http://dx.doi.org/10.1023/A:1008860819947 |
Título revista: | Biotechnology Techniques
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Título revista abreviado: | Biotechnol. Tech.
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ISSN: | 0951208X
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CODEN: | BTECE
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0951208X_v12_n9_p683_Terebiznik |
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Citas:
---------- APA ----------
Terebiznik, M.R., Zylberman, V., Buera, M.P. & Pilosof, A.M.R.
(1998)
. Stabilizing crude and ultrafiltrated α-amylase and α-glucosidase from Aspergillus oryzae by trehalose. Biotechnology Techniques, 12(9), 683-687.
http://dx.doi.org/10.1023/A:1008860819947---------- CHICAGO ----------
Terebiznik, M.R., Zylberman, V., Buera, M.P., Pilosof, A.M.R.
"Stabilizing crude and ultrafiltrated α-amylase and α-glucosidase from Aspergillus oryzae by trehalose"
. Biotechnology Techniques 12, no. 9
(1998) : 683-687.
http://dx.doi.org/10.1023/A:1008860819947---------- MLA ----------
Terebiznik, M.R., Zylberman, V., Buera, M.P., Pilosof, A.M.R.
"Stabilizing crude and ultrafiltrated α-amylase and α-glucosidase from Aspergillus oryzae by trehalose"
. Biotechnology Techniques, vol. 12, no. 9, 1998, pp. 683-687.
http://dx.doi.org/10.1023/A:1008860819947---------- VANCOUVER ----------
Terebiznik, M.R., Zylberman, V., Buera, M.P., Pilosof, A.M.R. Stabilizing crude and ultrafiltrated α-amylase and α-glucosidase from Aspergillus oryzae by trehalose. Biotechnol. Tech. 1998;12(9):683-687.
http://dx.doi.org/10.1023/A:1008860819947