Abstract:
The isolation, purification and analysis of the lipid A obtained from Mesorhizobium loti Ayac 1 BII strain is presented. Analysis of the carbohydrate moiety after acid hydrolysis by high-pH anion-exchange chromatography with pulse amperometric detection (HPAEC-PAD) showed the presence of glucosamine and galacturonic acid as the only sugar components. Gas chromatographice (GC) and GC/ mass spectrometric (MS) analysis of the fatty acids revealed the presence of 3-OH-C12:0; 3-OH-C13:0; 3-OH-C20:0 and 27-OH-C28:0 among the major hydroxylated species. In addition, C16:0, C17:0, C18:0 and C 20:0 were shown as main saturated fatty acids. Different polyacylated species were evidenced by thin layer chromatography of lipid A, allowing the purification of two fractions. Ultraviolet matrix-assisted laser desorption/ionization time-of-flight (UV-MALDI-TOF) MS analysis with different matrices, in the positive- and negative-ion mode, was performed. The fast moving component revealed the presence of hexa-acylated species, varying in the fatty acid composition. Species containing three 3-OH fatty acids and a 27-OH-C28:0 fatty acid were observed. Individual ions within this family differ by ±14 mass units. The slow moving component was enriched mainly in penta-acylated species. Among them, three subgroups were detected: the major one compatible with the sugar core bearing two 3-OH 20:0 fatty acids, a 3-OH 13:0 or a 3-OH 12:0 fatty acid, a 27-OH 28:0 fatty acid and one saturated fatty acid. Each signal differs in a C18:0 acyl unit from the corresponding hexa-acylated family. On the other hand, a subgroup bearing one 3-OH 20:0 fatty acid, one 27-OH 28:0 fatty acid and two non-polar fatty acids was shown. A minor subgroup compatible with structures containing two hydroxylated and three non-polar fatty acids was also detected. The results obtained showed that nor-harmane was an excellent matrix for charged lipid A structural studies in both, positive and negative ion modes. Copyright © 2006 John Wiley & Sons, Ltd.
Registro:
Documento: |
Artículo
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Título: | A matrix-assisted laser desorption/ionization mass spectrometry approach to the lipid A from Mesorhizobium loti |
Autor: | Casabuono, A.C.; D'Antuono, A.; Sato, Y.; Nonami, H.; Ugalde, R.; Lepek, V.; Erra-Balsells, R.; Couto, A.S. |
Filiación: | CIHIDECAR-CONICET, Departamento de Química Orgánica, Universidad de Buenos Aires, Argentina Faculty of Agriculture, Ehime University, Japan Instituto de Investigaciones Biotecnológicas, INTECH, CONICET, Buenos Aires, Argentina Departamento de Química Orgánica, F.C.E.y N., Ciudad Universitaria, 1428 Buenos Aires, Argentina
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Palabras clave: | fatty acid; galacturonic acid; glucosamine; hexuronic acid; lipid A; article; chemistry; mass fragmentography; mass spectrometry; methodology; physiology; Proteobacteria; thin layer chromatography; Chromatography, Thin Layer; Fatty Acids; Gas Chromatography-Mass Spectrometry; Glucosamine; Hexuronic Acids; Lipid A; Proteobacteria; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
Año: | 2006
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Volumen: | 20
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Número: | 14
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Página de inicio: | 2175
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Página de fin: | 2182
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DOI: |
http://dx.doi.org/10.1002/rcm.2575 |
Título revista: | Rapid Communications in Mass Spectrometry
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Título revista abreviado: | Rapid Commun. Mass Spectrom.
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ISSN: | 09514198
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CODEN: | RCMSE
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CAS: | galacturonic acid, 14982-50-4, 685-73-4; glucosamine, 3416-24-8, 4607-22-1; lipid A, 95991-05-2; Fatty Acids; galacturonic acid, 14982-50-4; Glucosamine, 3416-24-8; Hexuronic Acids; Lipid A
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09514198_v20_n14_p2175_Casabuono |
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Citas:
---------- APA ----------
Casabuono, A.C., D'Antuono, A., Sato, Y., Nonami, H., Ugalde, R., Lepek, V., Erra-Balsells, R.,..., Couto, A.S.
(2006)
. A matrix-assisted laser desorption/ionization mass spectrometry approach to the lipid A from Mesorhizobium loti. Rapid Communications in Mass Spectrometry, 20(14), 2175-2182.
http://dx.doi.org/10.1002/rcm.2575---------- CHICAGO ----------
Casabuono, A.C., D'Antuono, A., Sato, Y., Nonami, H., Ugalde, R., Lepek, V., et al.
"A matrix-assisted laser desorption/ionization mass spectrometry approach to the lipid A from Mesorhizobium loti"
. Rapid Communications in Mass Spectrometry 20, no. 14
(2006) : 2175-2182.
http://dx.doi.org/10.1002/rcm.2575---------- MLA ----------
Casabuono, A.C., D'Antuono, A., Sato, Y., Nonami, H., Ugalde, R., Lepek, V., et al.
"A matrix-assisted laser desorption/ionization mass spectrometry approach to the lipid A from Mesorhizobium loti"
. Rapid Communications in Mass Spectrometry, vol. 20, no. 14, 2006, pp. 2175-2182.
http://dx.doi.org/10.1002/rcm.2575---------- VANCOUVER ----------
Casabuono, A.C., D'Antuono, A., Sato, Y., Nonami, H., Ugalde, R., Lepek, V., et al. A matrix-assisted laser desorption/ionization mass spectrometry approach to the lipid A from Mesorhizobium loti. Rapid Commun. Mass Spectrom. 2006;20(14):2175-2182.
http://dx.doi.org/10.1002/rcm.2575