High-throughput transcriptome studies are breaking new ground to investigate the responses that organisms deploy in alternative environments. Nevertheless, much remains to be understood about the genetic basis of host plant adaptation. Here, we investigate genome-wide expression in the fly Drosophila buzzatii raised in different conditions. This species uses decaying tissues of cactus of the genus Opuntia as primary rearing substrate and secondarily, the necrotic tissues of the columnar cactus Trichocereus terscheckii. The latter constitutes a harmful host, rich in mescaline and other related phenylethylamine alkaloids. We assessed the transcriptomic responses of larvae reared in Opuntia sulphurea and T. terscheckii, with and without the addition of alkaloids extracted from the latter. Whole-genome expression profiles were massively modulated by the rearing environment, mainly by the presence of T. terscheckii alkaloids. Differentially expressed genes were mainly related to detoxification, oxidation-reduction and stress response; however, we also found genes involved in development and neurobiological processes. In conclusion, our study contributes new data onto the role of transcriptional plasticity in response to alternative rearing environments. © 2016 John Wiley & Sons Ltd.
Documento: | Artículo |
Título: | Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila |
Autor: | De Panis, D.N.; Padró, J.; Furió-Tarí, P.; Tarazona, S.; Milla Carmona, P.S.; Soto, I.M.; Dopazo, H.; Conesa, A.; Hasson, E. |
Filiación: | IEGEBA-CONICET UNiversidad de Buenos Aires FAcultad de Ciencias Exactas y Naturales Intendente Güiraldes 2160 Ciudad Universitaria (C1428 EHA) CABA Argentina Genomics of Gene Expression Lab Centro de Investigación Príncipe Felipe Eduardo Primo Yúfera 3 Valencia 46012 Spain Department of Applied Statistics, Operations Research and Quality Universitat Politècnica de València Camí de Vera Valencia 46022 Spain Laboratorio de Ecosistemas Marinos Fósiles Instituto de Estudios Andinos Don Pablo Groeber (CONICET-UBA) Intendente Güiraldes 2160 Ciudad Universitaria (C1428 EHA) CABA Argentina Microbiology and Cell Science Department Institute for Food and Agricultural Sciences University of Florida at Gainesville Gainesville 32603FL USA |
Palabras clave: | Alkaloids; Environment adaptation; Mescaline; Plasticity; RNA-Seq |
Año: | 2016 |
DOI: | http://dx.doi.org/10.1111/mec.13785 |
Título revista: | Molecular Ecology |
Título revista abreviado: | Mol. Ecol. |
ISSN: | 09621083 |
CODEN: | MOECE |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09621083_v_n_p_DePanis |