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De La Cal, C.; Lomniczi, A.; Mohn, C.E.; De Laurentiis, A.; Casal, M.; Chiarenza, A.; Paz, D.; McCann, S.M.; Rettori, V.; Elverdín, J.C. "Decrease in salivary secretion by radiation mediated by nitric oxide and prostaglandins" (2006) NeuroImmunoModulation. 13(1):19-27
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Abstract:

Objective: In the present work, we evaluated the effect of exposing the submandibular glands (SMG) to radiation, studying different functional parameters such as salivary secretion, nitric oxide (NO) production, reactive oxygen species formation, prostaglandin (PGE) content and apoptosis. Methods: We irradiated rats in the head and neck region with a single dose of γ-ray radiation of 15 Gy. Two hours after radiation, we measured norepinephrine- induced salivary secretion. After that, the SMG were dissected, and in this tissue, we measured the activity of NO synthase (NOS), the PGE content, the amount of reactive oxygen species, apoptotic cells and mitochondrial inducible NOS (iNOS) expression. Results: We found that radiation decreased salivary secretion when 10 and 30 μg/kg of norepinephrine was administered via the right femoral vein. We observed that iNOS activity was reduced and PGE content increased after radiation in SMG, indicating that NO and PGEs may participate in salivary secretion. The expression of mitochondrial NOS was increased after radiation leading to the formation of large amounts of NO that acts as a proapoptotic signal. In fact, we observed an augmentation in apoptotic cells. In this study, we also observed an increase in lipid peroxidation induced by radiation that may contribute to tissue damage. Conclusions: Our results indicate that radiation induced a decrease in salivary secretion and SMG iNOS activity, meanwhile the PGE content, the lipid peroxidation and apoptosis increased in the tissue. These modifications decrease salivary secretion. Copyright © 2006 S. Karger AG.

Registro:

Documento: Artículo
Título:Decrease in salivary secretion by radiation mediated by nitric oxide and prostaglandins
Autor:De La Cal, C.; Lomniczi, A.; Mohn, C.E.; De Laurentiis, A.; Casal, M.; Chiarenza, A.; Paz, D.; McCann, S.M.; Rettori, V.; Elverdín, J.C.
Filiación:Cátedra de Fisiología, Universidad de Buenos Aires, Buenos Aires, Argentina
Cátedra de Bioquímica, Facultad de Odontología, Universidad de Buenos Aires, Buenos Aires, Argentina
Centro de Estudios Farmacológicos Y Botánicos, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Oncología Angel H. Roffo, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento Biodiversidad Y Biología Experimental and IFIBYNE-CONICET, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Centro de Estudios Farmacológicos Y Botánicos (CEFyBO), Facultad de Medicina, UBA, Paraguay 2155, CP 1121, Buenos Aires, Argentina
Palabras clave:Apoptosis; Nitric oxide synthase; Submandibular glands; Xerostomia; inducible nitric oxide synthase; nitric oxide; nitric oxide synthase; noradrenalin; prostaglandin; reactive oxygen metabolite; animal experiment; animal tissue; apoptosis; article; controlled study; enzyme activity; female; femoral vein; gamma radiation; irradiation; lipid peroxidation; mitochondrion; nonhuman; priority journal; protein expression; radiation; rat; salivation; submandibular gland; tissue injury; Animals; Apoptosis; Disease Models, Animal; Down-Regulation; Epithelial Cells; Female; Head and Neck Neoplasms; Lipid Peroxidation; Nitric Oxide; Nitric Oxide Synthase Type II; Oxidative Stress; Prostaglandins; Radiotherapy; Rats; Saliva; Submandibular Gland; Xerostomia
Año:2006
Volumen:13
Número:1
Página de inicio:19
Página de fin:27
DOI: http://dx.doi.org/10.1159/000093194
Título revista:NeuroImmunoModulation
Título revista abreviado:NeuroImmunomodulation
ISSN:10217401
CODEN:NROIE
CAS:inducible nitric oxide synthase, 501433-35-8; nitric oxide, 10102-43-9; nitric oxide synthase, 125978-95-2; noradrenalin, 1407-84-7, 51-41-2; Nitric Oxide, 10102-43-9; Nitric Oxide Synthase Type II, 1.14.13.39; Prostaglandins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10217401_v13_n1_p19_DeLaCal

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Citas:

---------- APA ----------
De La Cal, C., Lomniczi, A., Mohn, C.E., De Laurentiis, A., Casal, M., Chiarenza, A., Paz, D.,..., Elverdín, J.C. (2006) . Decrease in salivary secretion by radiation mediated by nitric oxide and prostaglandins. NeuroImmunoModulation, 13(1), 19-27.
http://dx.doi.org/10.1159/000093194
---------- CHICAGO ----------
De La Cal, C., Lomniczi, A., Mohn, C.E., De Laurentiis, A., Casal, M., Chiarenza, A., et al. "Decrease in salivary secretion by radiation mediated by nitric oxide and prostaglandins" . NeuroImmunoModulation 13, no. 1 (2006) : 19-27.
http://dx.doi.org/10.1159/000093194
---------- MLA ----------
De La Cal, C., Lomniczi, A., Mohn, C.E., De Laurentiis, A., Casal, M., Chiarenza, A., et al. "Decrease in salivary secretion by radiation mediated by nitric oxide and prostaglandins" . NeuroImmunoModulation, vol. 13, no. 1, 2006, pp. 19-27.
http://dx.doi.org/10.1159/000093194
---------- VANCOUVER ----------
De La Cal, C., Lomniczi, A., Mohn, C.E., De Laurentiis, A., Casal, M., Chiarenza, A., et al. Decrease in salivary secretion by radiation mediated by nitric oxide and prostaglandins. NeuroImmunomodulation. 2006;13(1):19-27.
http://dx.doi.org/10.1159/000093194