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Abstract:

A part of sperm glycosidase activities was detected as detergent-insoluble after sequential extractions with Triton X-100. Sixty per cent of total β-glucuronidase activity was found in the detergent-insoluble fraction. This portion of β-glucuronidase was resistant to extractions in the presence of 1 M KCl, chaotropic agents, colchicine or cytochalasine B, being only partially solubilized by 3 M KCl or DNAse I treatment. Results demonstrate that β-glucuronidase is tightly associated to the Triton X-100 resistant fraction.

Registro:

Documento: Artículo
Título:Human sperm β-glucuronidase is poorly extractable by triton X-100
Autor:Brandelli, A.; Miranda, P.V.; Tezón, J.G.
Filiación:Departamento de Bioquimica, Instituto de Biociências, Univ. Federal do Rio Grande do Sul, 90050-170 Porto Alegre, Brazil
Inst. de Biol. y Med. Experimental, CONICET, 1428 Buenos Aires, Argentina
Palabras clave:Cytoskeleton; Detergent; Enzyme; Glycosidase; Sperm; beta glucuronidase; colchicine; cytochalasin B; deoxyribonuclease I; potassium chloride; triton x 100; article; controlled study; enzyme activity; human; human cell; male; normal human; protein isolation; solubilization; sperm
Año:1996
Volumen:20
Número:5
Página de inicio:351
Página de fin:354
DOI: http://dx.doi.org/10.1006/cbir.1996.0040
Título revista:Cell Biology International
Título revista abreviado:CELL BIOL. INT.
ISSN:10656995
CODEN:CBIIE
CAS:beta glucuronidase, 9001-45-0; colchicine, 64-86-8; cytochalasin B, 14930-96-2; deoxyribonuclease I, 9003-98-9; potassium chloride, 7447-40-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10656995_v20_n5_p351_Brandelli

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Citas:

---------- APA ----------
Brandelli, A., Miranda, P.V. & Tezón, J.G. (1996) . Human sperm β-glucuronidase is poorly extractable by triton X-100. Cell Biology International, 20(5), 351-354.
http://dx.doi.org/10.1006/cbir.1996.0040
---------- CHICAGO ----------
Brandelli, A., Miranda, P.V., Tezón, J.G. "Human sperm β-glucuronidase is poorly extractable by triton X-100" . Cell Biology International 20, no. 5 (1996) : 351-354.
http://dx.doi.org/10.1006/cbir.1996.0040
---------- MLA ----------
Brandelli, A., Miranda, P.V., Tezón, J.G. "Human sperm β-glucuronidase is poorly extractable by triton X-100" . Cell Biology International, vol. 20, no. 5, 1996, pp. 351-354.
http://dx.doi.org/10.1006/cbir.1996.0040
---------- VANCOUVER ----------
Brandelli, A., Miranda, P.V., Tezón, J.G. Human sperm β-glucuronidase is poorly extractable by triton X-100. CELL BIOL. INT. 1996;20(5):351-354.
http://dx.doi.org/10.1006/cbir.1996.0040