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Dujardin, G.; Lafaille, C.; de la Mata, M.; Marasco, L.E.; Muñoz, M.J.; Le Jossic-Corcos, C.; Corcos, L.; Kornblihtt, A.R. "How Slow RNA Polymerase II Elongation Favors Alternative Exon Skipping" (2014) Molecular Cell. 54(4):683-690
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Abstract:

Splicing is functionally coupled to transcription, linking the rate of RNA polymerase II (Pol II) elongation and the ability of splicing factors to recognize splice sites (ss) of various strengths. In most cases, slow Pol II elongation allows weak splice sites to be recognized, leading to higher inclusion of alternative exons. Using CFTR alternative exon 9 (E9) as a model, we show here that slowing down elongation can also cause exon skipping by promoting the recruitment of the negative factor ETR-3 onto the UG-repeat at E9 3' splice site, which displaces the constitutive splicing factor U2AF65 from the overlapping polypyrimidine tract. Weakening of E9 5' ss increases ETR-3 binding at the 3' ss and subsequent E9 skipping, whereas strengthening of the 5' ss usage has the opposite effect. This indicates that a delay in the cotranscriptional emergence of the 5' ss promotes ETR-3 recruitment and subsequent inhibition of E9 inclusion. © 2014 Elsevier Inc.

Registro:

Documento: Artículo
Título:How Slow RNA Polymerase II Elongation Favors Alternative Exon Skipping
Autor:Dujardin, G.; Lafaille, C.; de la Mata, M.; Marasco, L.E.; Muñoz, M.J.; Le Jossic-Corcos, C.; Corcos, L.; Kornblihtt, A.R.
Filiación:Laboratorio de Fisiología y Biología Molecular, Departamento de Fisiología, Biología Molecular y Celular, IFIBYNE-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, C1428EHA Buenos Aires, Argentina
Inserm U1078-ECLA Team, Faculty of Medicine, 22 Avenue Camille Desmoulins, 29238 Brest Cedex 3, France
Palabras clave:RNA polymerase II; 3' untranslated region; 5' untranslated region; alternative RNA splicing; amplicon; article; CACO 2 cell line; chromatin structure; controlled study; enzyme inhibition; enzyme phosphorylation; exon; exon skipping; expression vector; human; human cell; point mutation; protein expression; reverse transcription polymerase chain reaction; transcription elongation; ultraviolet irradiation; Western blotting; Alternative Splicing; Binding Sites; Caco-2 Cells; Cystic Fibrosis Transmembrane Conductance Regulator; Exons; HEK293 Cells; Humans; Models, Genetic; Nerve Tissue Proteins; Nuclear Proteins; Ribonucleoproteins; RNA Polymerase II; RNA Splice Sites; RNA-Binding Proteins; Transcription, Genetic
Año:2014
Volumen:54
Número:4
Página de inicio:683
Página de fin:690
DOI: http://dx.doi.org/10.1016/j.molcel.2014.03.044
Título revista:Molecular Cell
Título revista abreviado:Mol. Cell
ISSN:10972765
CODEN:MOCEF
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10972765_v54_n4_p683_Dujardin

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Citas:

---------- APA ----------
Dujardin, G., Lafaille, C., de la Mata, M., Marasco, L.E., Muñoz, M.J., Le Jossic-Corcos, C., Corcos, L.,..., Kornblihtt, A.R. (2014) . How Slow RNA Polymerase II Elongation Favors Alternative Exon Skipping. Molecular Cell, 54(4), 683-690.
http://dx.doi.org/10.1016/j.molcel.2014.03.044
---------- CHICAGO ----------
Dujardin, G., Lafaille, C., de la Mata, M., Marasco, L.E., Muñoz, M.J., Le Jossic-Corcos, C., et al. "How Slow RNA Polymerase II Elongation Favors Alternative Exon Skipping" . Molecular Cell 54, no. 4 (2014) : 683-690.
http://dx.doi.org/10.1016/j.molcel.2014.03.044
---------- MLA ----------
Dujardin, G., Lafaille, C., de la Mata, M., Marasco, L.E., Muñoz, M.J., Le Jossic-Corcos, C., et al. "How Slow RNA Polymerase II Elongation Favors Alternative Exon Skipping" . Molecular Cell, vol. 54, no. 4, 2014, pp. 683-690.
http://dx.doi.org/10.1016/j.molcel.2014.03.044
---------- VANCOUVER ----------
Dujardin, G., Lafaille, C., de la Mata, M., Marasco, L.E., Muñoz, M.J., Le Jossic-Corcos, C., et al. How Slow RNA Polymerase II Elongation Favors Alternative Exon Skipping. Mol. Cell. 2014;54(4):683-690.
http://dx.doi.org/10.1016/j.molcel.2014.03.044