Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor


Aquaculture activity has grown remarkably in the last years, crustaceans being the most profitable products because of their high prices. Feeding costs represent a large portion of total operating costs in the aquaculture industry. In this context, the objective of the present study was to evaluate the effect of food restriction on the reproductive performance of redclaw crayfish Cherax quadricarinatus. Females at the onset of sexual maturity were stocked with males and were fed daily a formulated diet at 1.5% (control group) and 0.5% (restricted group) of their mean weight. The experimental period lasted 105 days. The percentage of ovigerous females and broods successfully hatched tended to be higher in restricted females compared with control females, but egg volume and weight did not differ between both experimental groups. However, the lipid concentration of rematuring ovaries was lower in restricted females than control females, suggesting that food restriction affects the amount of reserves transferred to the ovaries during vitellogenesis. The nutritional state of females was independent of the amount of food provided, as reflected by similar growth parameters and biochemical composition of the hepatopancreas in control and restricted females. Based on these results, we conclude that it is possible to reduce the feeding rate of C. quadricarinatus females to one-third of its original value without affecting their reproductive performance and somatic growth, at least for a 105-day period. This finding provides a useful tool for commercial producers to decrease production costs and improve water quality in culture systems. © 2016 John Wiley & Sons Ltd


Documento: Artículo
Título:Effect of food restriction on female reproductive performance in the redclaw crayfish Cherax quadricarinatus (Parastacidae, Decapoda)
Autor:Castillo Díaz, F.; Tropea, C.; Stumpf, L.; López Greco, L.S.
Filiación:Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA, CONICET-UBA), Facultad de Ciencias Exactas y Naturales, Departamento de Biodiversidad y Biología Experimental, Biología de la Reproducción y el Crecimiento de Crustáceos Decápodos, Buenos Aires, Argentina
Palabras clave:biochemical composition; Cherax quadricarinatus; food restriction; reproductive performance; somatic growth; aquaculture industry; artificial diet; biochemical composition; commercial activity; cost analysis; crayfish; experimental study; feeding behavior; female; food; growth; hatching; reproductive strategy; sexual maturity; stocking density; Astacoidea; Cherax quadricarinatus; Crustacea; Decapoda (Crustacea); Parastacidae
Página de inicio:4228
Página de fin:4237
Título revista:Aquaculture Research
Título revista abreviado:Aquac. Res.


  • Al-Mohanna, S.Y., Nott, J.A., R-cells and the digestive cycle in Penaeus semisulcatus (Crustacea: Decapoda) (1987) Marine Biology, 95, pp. 129-137
  • Attard, J., Hudon, C., Embryonic development and energetic investment in egg production in relation to size of female lobster (Homarus americanus) (1987) Canadian Journal of Fisheries and Aquatic Sciences, 44, pp. 1157-1164
  • Barki, A., Karplus, I., Manor, R., Parnes, S., Aflalo, E.D., Sagi, A., Growth of redclaw crayfish (Cherax quadricarinatus) in a three-dimensional compartments system: Does a neighbor matter? (2006) Aquaculture, 252, pp. 348-355
  • Berillis, P., Simon, C., Mente, E., Sofos, F., Karapanagiotidis, I.T., A novel image processing method to determine the nutritional condition of lobsters (2013) Micron, 45, pp. 140-144
  • Bradford, M.M., A refined and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding (1976) Analytical Biochemistry, 72, pp. 248-254
  • Bromage, N., Broodstock management and seed quality-general considerations (1995) Broodstock Management and Egg and Larval Quality, pp. 1-24. , In, (ed. by, N.R. Bromage, &, R.J. Roberts, Blackwell Science, Oxford, UK
  • Caceci, T., Neck, K.F., Lewis, D.H., Sis, R.F., Ultrastructure of the hepatopancreas of the pacific white shrimp Penaeus vannamei (Crustacea: Decapoda) (1988) Journal of the Marine Biological Association of the United Kingdom, 68, pp. 323-337
  • Calvo, N.S., Stumpf, L., Pietrokovsky, S., López Greco, L.S., Early and late effects of feed restriction on survival, growth and hepatopancreas structure in juveniles of the red claw crayfish Cherax quadricarinatus (2011) Aquaculture, 319, pp. 355-362
  • Calvo, N.S., Tropea, C., Anger, K., López Greco, L.S., Starvation resistance in juvenile freshwater crayfish (2012) Aquatic Biology, 16, pp. 287-297
  • Castille, F.L., Lawrence, A.L., Relationship between maturation and biochemical composition of the gonads and digestive glands of the shrimps Penaeus aztecus Ives and Penaeus setiferus (L.) (1989) Journal of Crustacean Biology, 9, pp. 202-211
  • Clarke, A., Egg size and egg composition in polar shrimps (Caridea: Decapoda) (1993) Journal of Experimental Marine Biology and Ecology, 168, pp. 189-203
  • Coutteau, P., Geurden, I., Camara, M.R., Bergot, P., Sorgeloos, P., Review on the dietary effects of phospholipids in fish and crustacean larviculture (1997) Aquaculture, 155, pp. 149-164
  • Evans, L.H., Jussila, J., Freshwater crayfish growth under culture conditions: proposition for a standard reporting approach (1997) Journal of the World Aquaculture Society, 28, pp. 11-18
  • Evans, L.H., Fan, A., Finn, S.P., Dawson, S.A., Siva, C.J., Lee, I.R., Nutritional status assessment studies in the freshwater crayfish, Cherax tenuimanus (1992) Proceedings of the Aquaculture Nutrition Workshop, pp. 87-91. , In, (ed. by, G.L. Allan, &, W. Dall, Salamander Bay, Australia
  • (2014) The State of World Fisheries and Aquaculture, 2012, , Food and Agriculture Organization (FAO), Rome, Italy
  • Folch, J., Lees, M., Sloane-Stanley, H., A simple method for the isolation and purification of total lipids from animal tissue (1957) The Journal of Biological Chemistry, 226, pp. 497-509
  • Franceschini-Vicentini, I.B., Ribeiro, K., Papa, L.P., Marques Junior, J.M., Vicentini, C.A., Moraes Valenti, P.M.C.M., Histoarchitectural features of the hepatopancreas of the Amazon river prawn Macrobrachium amazonicum (2009) International Journal of Morphology, 27, pp. 121-128
  • Frings, C.S., Dunn, R.T., A colorimetric method for determination of total serum lipids based on the sulfo-phospho-vanillin reaction (1970) American Journal of Clinical Pathology, 53, pp. 89-91
  • Galois, R.G., Changes in the tissular lipidic composition during vitellogenesis in the shrimp Penaeus indicus Milne Edwards (1984) Journal of Experimental Marine Biology and Ecology, 84, pp. 155-166
  • García-Ulloa, G.M., (2000) Fundamentos de nutrición acuícola, , Universidad Autónoma de Guadalajara, México
  • García-Ulloa, G.M., López-Chavarín, H.M., Rodríguez-González, H., Villarreal-Colmenares, H., Growth of redclaw crayfish Cherax quadricarinatus (von Martens 1868) (Decapoda: Parastacidae) juveniles fed isoproteic diets with partial or total substitution of fish meal by soya bean meal: preliminary study (2003) Aquaculture Nutrition, 9, pp. 25-31
  • García-Ulloa, M., Rodríguez, H., Ogura, T., Egg quality of two prawn species (Palaemonidae) of the genus Macrobrachium (M. rosenbergii, De Man 1879, y M. tenellum, Smith, 1871) varying the brood stock diet: morphometric indexes (2004) Avances en Investigación Agropecuaria, 8, pp. 17-27
  • Gibson, R., Barker, P.L., The decapod hepatopancreas (1979) Oceanography and Marine Biology: An Annual Review, 17, pp. 285-346
  • Grant, A., Tyler, P.A., The analysis of data in studies of invertebrate reproduction. I. Introduction and statistical analysis of gonad indices and maturity indices (1983) International Journal of Invertebrate Reproduction, 6, pp. 259-269
  • Gutiérrez, M.L., Rodríguez, E.M., Effect of protein source on growth of early juvenile redclaw crayfish Cherax quadricarinatus (Decapoda, Parastacidae) (2010) Freshwater Crayfish, 17, pp. 23-29
  • Harrison, E.K., Broodstock nutrition and maturation diets (1997) Crustacean Nutrition, , In, (ed. by, E. Harrison, K.L.R. D'Abramo, D.E. Conklin, &, D.M. Akiyama, Baton Rouge, Louisiana, USA
  • Hernández-Vergara, M.P., Rouse, D.B., Olvera-Novoa, M.A., Davis, D.A., Effects of dietary lipid level and source on growth and proximate composition of juvenile redclaw (Cherax quadricarinatus) reared under semi-intensive culture conditions (2003) Aquaculture, 223, pp. 107-115
  • Herring, P.J., Size, density and lipid content of some decapod eggs (1974) Deep-Sea Research, 21, pp. 91-94
  • Hines, A.H., Allometric constraints and variables of reproductive effort in Brachyuran crabs (1982) Marine Biology, 69, pp. 309-320
  • Icely, J.D., Nott, J.A., Digestion and absorption: digestive system and associated organs (1992) Microscopic Anatomy of Invertebrates: Decapod, Crustacea, 10, pp. 147-201. , In, (ed. by, F.W. Harrison, &, A.G. Humes, Wiley-Liss, New York, NY, USA
  • Johnston, D.J., Alexander, C.G., Yellowhees, D., Epithelial cytology and function in the digestive gland of Thenus orientalis (Decapoda, Scyllaridae) (1998) Journal of Crustacean Biology, 18, pp. 271-278
  • Jones, C.M., (1997) The Biology and Aquaculture Potential of the Tropical Freshwater Crayfish Cherax quadricarinatus, , Department of Primary Industries, Queensland
  • Jones, P.L., Obst, J.H., Effects of starvation and subsequent refeeding on the size and nutrient content of the hepatopancreas of Cherax destructor (Decapoda: Parastacidae) (2000) Journal of Crustacean Biology, 20, pp. 431-441
  • Jones, C.M., Ruscoe, I.M., Assessment of stocking size and density in the production of redclaw crayfish, Cherax quadricarinatus (von Martens) (Decapoda: Parastacidae), cultured under earthen pond conditions (2000) Aquaculture, 189, pp. 63-71
  • Jones, C.M., Ruscoe, I.M., Assessment of five shelter types in the production of redclaw crayfish Cherax quadricarinatus (Decapoda: Parastacidae) under earthen pond conditions (2001) Journal of the World Aquaculture Society, 32, pp. 41-52
  • Jussila, J., Evans, L.H., Growth and condition of marron Cherax tenuimanus fed pelleted diets of different stability (1998) Aquaculture Nutrition, 4, pp. 143-149
  • Lawrence, C., Jones, C.M., Cherax (2002) Biology of Freshwater Crayfish, pp. 635-669. , In, (ed. by, D.M. Holdich, Blackwell Science, London
  • López Greco, L.S., Vazquez, F.J., Rodríguez, E., Morphology of the male reproductive system and spermatophore formation in the freshwater ‘red claw’ crayfish Cherax quadricarinatus (von Martens, 1898) (Decapoda, Parastacidae) (2007) Acta Zoologica, 88, pp. 223-229
  • Luchini, L., Panné Huidobro, S., (2008) Perspectivas en Acuicultura: nivel mundial, regional y local, , Dirección de Acuicultura; Secretaría de Agricultura, Ganadería, Pesca y Alimentos (SAGPyA), Subsecretaría de Pesca y Acuicultura, Argentina
  • Masser, M.P., Rouse, D.B., (1997) Australian Red Claw Crayfish, , SRAC Publication, 244., Southern Regional Aquaculture Center, Auburn University, Auburn, AL
  • McClain, W.R., Growth of crawfish Procambarus clarkii as a function of density and food resources (1995) Journal of the World Aquaculture Society, 26, pp. 24-28
  • McRae, T.G., Mitchell, B.D., Studies on ovarian development in the yabby, Cherax albidus Clark (1995) 10th Int. Sym. Of Astacology: Freshwater Crayfish, 10, pp. 521-531. , In, (ed. by, D.R. Fielder, A.M.M. Richardson, Louisiana State University, Baton Rouge, LA
  • Meade, M.E., Watts, S.A., Toxicity of ammonia, nitrite, and nitrate to juvenile Australian crayfish, Cherax quadricarinatus (1995) Journal of Shellfish Research, 14, pp. 341-346
  • Mourente, G., Medina, A., González, S., Rodríguez, A., Changes in lipid class and fatty acid contents in the ovary and midgut gland of the female fiddler crab Uca tangeri (Decapoda, Ocypodidae) during maturation (1994) Marine Biology, 121, pp. 187-197
  • Pavasovic, A., Anderson, A.J., Mather, P.B., Richardson, N.A., Influence of dietary protein on digestive enzyme activity, growth and tail muscle composition in redclaw crayfish, Cherax quadricarinatus (von Martens) (2007) Aquaculture Research, 38, pp. 644-652
  • Rodríguez-González, H., García-Ulloa, M., Hernández-Llamas, A., Villarreal, H., Effect of dietary protein level on spawning and egg quality of redclaw crayfish Cherax quadricarinatus (2006) Aquaculture, 257, pp. 412-419
  • Rodríguez-González, H., Hernández-Llamas, A., Villarreal, H., Saucedo, P.E., García-Ulloa, M., Rodríguez-Jaramillo, C., Gonadal development and biochemical composition of female crayfish Cherax quadricarinatus (Decapoda: Parastacidae) in relation to the Gonadosomatic Index at first maturation (2006) Aquaculture, 254, pp. 637-645
  • Rodríguez-González, H., Villarreal, H., Hernández-Llamas, A., García-Ulloa, M., Vázquez-Boucard, C., Serrano-Pinto, V., Effect of dietary protein-to-energy ratio on reproduction in female redclaw crayfish Cherax quadricarinatus (2011) Aquaculture Research, 42, pp. 1840-1847
  • Rodríguez-González, H., Hernández-Llamas, A., García-Ulloa, M., Racotta, I.S., Montoya-Mejía, M., Villarreal, H., Effect of protein and lipids levels in diets for female red claw crayfish Cherax quadricarinatus on quality of offspring (juvenile) with emphasis on growth performance, biochemical composition and stress resistance to low oxygen, high ammonia and salinity (2014) Aquaculture Nutrition, 20, pp. 557-565
  • Sacristán, H.J., Ansaldo, M., Franco Tadic, L.M., Fernández Giménez, A.V., López Greco, L.S., Long-term starvation and posterior feeding effects on biochemical and physiological responses of midgut gland of juveniles of Cherax quadricarinatus (Parastacidae (2016) PLoS ONE, 11 (3)
  • Saoud, I.P., Ghanawi, J., Molting, reproductive biology, and hatchery management of Redclaw Crayfish Cherax quadricarinatus (von Martens 1868) (2012) Aquaculture, 358-359, pp. 183-195
  • Saoud, I.P., Garza De Yta, A., Ghanawi, J., A review of nutritional biology and dietary requirements of redclaw crayfish Cherax quadricarinatus (von Martens 1868) (2012) Aquaculture Nutrition, 18, pp. 349-368
  • Shiau, S.Y., Nutrient requirements of penaeid shrimps (1998) Aquaculture, 164, pp. 77-93
  • Simon, C.J., Digestive enzyme response to natural and formulated diets in cultured juvenile spiny lobster, Jasus edwardsii (2009) Aquaculture, 294, pp. 271-281
  • Sokal, R.R., Rohlf, F.J., (1995) Biometry: The Principles and Practice of Statistics in Biological Research, , 3rd edn, W. H. Freeman and Co., New York
  • Sousa, L.G., Petriella, A.M., Histology of the hepatopancreas of the freshwater prawn Palaemonetes argentinus (Crustacea, Caridea) (2000) Biocell, 24, pp. 189-195
  • Stumpf, L., Calvo, N.S., Díaz, F.C., Valenti, W.C., López Greco, L.S., Effect of intermittent feeding on growth in early juveniles of the crayfish Cherax quadricarinatus (2011) Aquaculture, 319, pp. 98-104
  • Stumpf, L., Castillo Díaz, F., Viau, V.E., Valenti, W.C., López Greco, L.S., Effect of food shortage on growth, energetic reserves mobilization, and water quality in juveniles of the redclaw crayfish, Cherax quadricarinatus, reared in groups (2014) Journal of Crustacean Biology, 34, pp. 639-646
  • Thompson, K.R., Muzinic, L.A., Engler, L.S., Webster, C.D., Evaluation of practical diets containing different protein levels, with or without fish meal, for juvenile Australian red claw crayfish (Cherax quadricarinatus) (2005) Aquaculture, 244, pp. 241-249
  • Tropea, C., Arias, M., Calvo, N.S., López Greco, L.S., Influence of female size on offspring quality of the freshwater crayfish Cherax quadricarinatus (Parastacidae: Decapoda) (2012) Journal of the Crustacean Biology, 32, pp. 883-890
  • Van Handel, E., Estimation of glycogen in small amounts of tissue (1965) Analytical Biochemistry, 11, pp. 256-265
  • Vazquez, F.J., Tropea, C., López Greco, L.S., Development of the female reproductive system in the freshwater crayfish Cherax quadricarinatus (Decapoda, Parastacidae) (2008) Invertebrate Biology, 127, pp. 433-443
  • Vogt, G., Storch, V., Quinitio, E.T., Pascual, F.P., Midgut gland as monitor organ for the nutritional value of diets in Penaeus monodon (Decapoda) (1985) Aquaculture, 48, pp. 1-12


---------- APA ----------
Castillo Díaz, F., Tropea, C., Stumpf, L. & López Greco, L.S. (2017) . Effect of food restriction on female reproductive performance in the redclaw crayfish Cherax quadricarinatus (Parastacidae, Decapoda). Aquaculture Research, 48(8), 4228-4237.
---------- CHICAGO ----------
Castillo Díaz, F., Tropea, C., Stumpf, L., López Greco, L.S. "Effect of food restriction on female reproductive performance in the redclaw crayfish Cherax quadricarinatus (Parastacidae, Decapoda)" . Aquaculture Research 48, no. 8 (2017) : 4228-4237.
---------- MLA ----------
Castillo Díaz, F., Tropea, C., Stumpf, L., López Greco, L.S. "Effect of food restriction on female reproductive performance in the redclaw crayfish Cherax quadricarinatus (Parastacidae, Decapoda)" . Aquaculture Research, vol. 48, no. 8, 2017, pp. 4228-4237.
---------- VANCOUVER ----------
Castillo Díaz, F., Tropea, C., Stumpf, L., López Greco, L.S. Effect of food restriction on female reproductive performance in the redclaw crayfish Cherax quadricarinatus (Parastacidae, Decapoda). Aquac. Res. 2017;48(8):4228-4237.