Artículo

Piccinni, F.E.; Ontañon, O.M.; Ghio, S.; Sauka, D.H.; Talia, P.M.; Rivarola, M.L.; Valacco, M.P.; Campos, E. "Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates" (2019) Journal of Applied Microbiology. 126(3):811-825
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Abstract:

Aims: Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value-added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost-efficient enzymatic cocktails. Methods and Results: We identified 205 potential CAZymes in the genome of Cellulomonas sp. B6, 91 of which were glycoside hydrolases (GH). By secretome analysis of supernatants from cultures in either extruded wheat straw (EWS), grinded sugar cane straw (SCR) or carboxymethylcellulose (CMC), we identified which proteins played a role in lignocellulose deconstruction. Growth on CMC resulted in the secretion of two exoglucanases (GH6 and GH48) and two GH10 xylanases, while growth on SCR or EWS resulted in the identification of a diversity of CAZymes. From the 32 GHs predicted to be secreted, 22 were identified in supernatants from EWS and/or SCR cultures, including endo- and exoglucanases, xylanases, a xyloglucanase, an arabinofuranosidase/β-xylosidase, a β-glucosidase and an AA10. Surprisingly, among the xylanases, seven were GH10. Conclusions: Growth of Cellulomonas sp. B6 on lignocellulosic biomass induced the secretion of a diverse repertoire of CAZymes. Significance and Impact of the Study: Cellulomonas sp. B6 could serve as a source of lignocellulose-degrading enzymes applicable to bioprocessing and biotechnological industries. © 2018 The Society for Applied Microbiology

Registro:

Documento: Artículo
Título:Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
Autor:Piccinni, F.E.; Ontañon, O.M.; Ghio, S.; Sauka, D.H.; Talia, P.M.; Rivarola, M.L.; Valacco, M.P.; Campos, E.
Filiación:Instituto de Agrobiotecnología y Biología Molecular (IABIMO), Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de investigaciones Científicas y Tecnológicas (CONICET), Los Reseros y Nicolas Repetto (1686), Hurlingham, Buenos Aires, Argentina
Instituto de Microbiología y Zoología Agrícola, Centro de Investigación en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto Nacional de Tecnología Agropecuaria (INTA), Los Reseros y Nicolas Repetto (1686), Hurlingham, Buenos Aires, Argentina
Facultad de Ciencias Exactas y Naturales, Centro de Estudios Químicos y Biológicos por Espectrometría de Masa (CEQUIBIEM), Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina
Palabras clave:cazymes; cellulomonas; gh10; glucanases; glycosyl hydrolases; lignocellulose; secretome; xylanases; arabinofuranosidase; bacterial DNA; bacterial enzyme; bacterial RNA; carboxymethylcellulose; exoglucanase; glucan synthase; glycosidase; lignocellulose; RNA 16S; unclassified drug; xylan 1,4 beta xylosidase; xylan endo 1,3 beta xylosidase; xyloglucanase; bacterium; biofuel; biotechnology; biotransformation; cellulose; crop residue; enzyme activity; genome; lignin; secretion; straw; Article; bacterial genome; bacterial growth; bacterium culture; biomass; Cellulomonas; Cellulomonas aerilata; Cellulomonas biazotea; Cellulomonas bogoriensis; Cellulomonas carbonis; Cellulomonas cellasea; Cellulomonas chitinilytica; Cellulomonas composti; Cellulomonas denverensis; Cellulomonas fimi; Cellulomonas flavigena; Cellulomonas gelida; Cellulomonas hominis; Cellulomonas humilata; Cellulomonas iranensis; Cellulomonas marina; Cellulomonas massiliensis; Cellulomonas oligotrophica; Cellulomonas pakistanensis; Cellulomonas phragmiteti; Cellulomonas soli; Cellulomonas terrae; Cellulomonas uda; Cellulomonas xylanilytica; controlled study; DNA DNA hybridization; enzyme activity; enzyme substrate; Micrococcus luteus; nonhuman; phylogeny; protein secretion; RNA sequence; sugarcane; Cellulomonas; Cellulomonas sp.; Saccharum; Triticum aestivum
Año:2019
Volumen:126
Número:3
Página de inicio:811
Página de fin:825
DOI: http://dx.doi.org/10.1111/jam.14176
Título revista:Journal of Applied Microbiology
Título revista abreviado:J. Appl. Microbiol.
ISSN:13645072
CODEN:JAMIF
CAS:arabinofuranosidase, 138263-79-3; carboxymethylcellulose, 8050-38-2, 9000-11-7, 9004-32-4, 9050-04-8; glucan synthase, 55126-98-2, 74191-29-0; glycosidase, 9032-92-2; lignocellulose, 11132-73-3; xylan 1,4 beta xylosidase, 9025-53-0; xylan endo 1,3 beta xylosidase, 37278-89-0, 9025-55-2
Registro:http://digital.bl.fcen.uba.ar/collection/paper/document/paper_13645072_v126_n3_p811_Piccinni

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Citas:

---------- APA ----------
Piccinni, F.E., Ontañon, O.M., Ghio, S., Sauka, D.H., Talia, P.M., Rivarola, M.L., Valacco, M.P.,..., Campos, E. (2019) . Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates. Journal of Applied Microbiology, 126(3), 811-825.
http://dx.doi.org/10.1111/jam.14176
---------- CHICAGO ----------
Piccinni, F.E., Ontañon, O.M., Ghio, S., Sauka, D.H., Talia, P.M., Rivarola, M.L., et al. "Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates" . Journal of Applied Microbiology 126, no. 3 (2019) : 811-825.
http://dx.doi.org/10.1111/jam.14176
---------- MLA ----------
Piccinni, F.E., Ontañon, O.M., Ghio, S., Sauka, D.H., Talia, P.M., Rivarola, M.L., et al. "Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates" . Journal of Applied Microbiology, vol. 126, no. 3, 2019, pp. 811-825.
http://dx.doi.org/10.1111/jam.14176
---------- VANCOUVER ----------
Piccinni, F.E., Ontañon, O.M., Ghio, S., Sauka, D.H., Talia, P.M., Rivarola, M.L., et al. Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates. J. Appl. Microbiol. 2019;126(3):811-825.
http://dx.doi.org/10.1111/jam.14176