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Abstract:

Species of sea hares have been recognized traditionally based on morphological traits, mainly the radula, external coloration, and reproductive anatomy. However, recent studies have shown substantial color variation in some sea slug species. Molecular data have been successfully used to differentiate morphologically similar species of “opisthobranchs” and resolve questions on the taxonomic value of color. The objective of this paper is to use molecular data in an attempt to elucidate whether specimens of Aplysia brasiliana with distinct colorations and morphologies are actually the same species. To this end, DNA from 14 specimens of A. brasiliana was extracted, including five specimens identified as a distinct morphotype from typical A. brasiliana. Although the two morphotypes have consistent differences in their external morphology and radula, the molecular data confirmed that there are no significant genetic differences between them. This is another example of the need to re-evaluate taxonomic decisions based on morphology in light of molecular evidence. © 2014, Springer-Verlag Berlin Heidelberg and AWI.

Registro:

Documento: Artículo
Título:It’s not what it looks like: molecular data fails to substantiate morphological differences in two sea hares (Mollusca, Heterobranchia, Aplysiidae) from southern Brazil
Autor:Saad, L.O.; Cunha, C.M.; Colpo, K.D.; Valdés, Á.
Filiación:Universidade Estadual Paulista (UNESP)-Câmpus do Litoral Paulista, Praça Infante Dom Henrique s/no, Bairro: Parque Bitaru, São Vicente, SP 11330-900, Brazil
Museu de Zoologia (MZSP), Universidade de São Paulo, Avenida Nazaré, 481, Ipiranga, São Paulo, SP 04263-000, Brazil
Department of Biodiversity and Experimental Biology, FCEyN, CONICET and Biology of Reproduction and Growth in Crustaceans, University of Buenos Aires, CP: 1428EGA, Buenos Aires, Argentina
Department of Biological Sciences, California State Polytechnic University, 3801, West Temple Avenue, Pomona, CA 91768-4032, United States
Palabras clave:16S; Aplysia; CO1; Morphological variation; anatomy; color; genetic analysis; molecular analysis; mollusc; morphology; morphotype; Brazil; Anaspidea; Aplysia; Aplysiidae; Heterobranchia; Mollusca
Año:2014
Volumen:68
Número:4
Página de inicio:523
Página de fin:530
DOI: http://dx.doi.org/10.1007/s10152-014-0408-1
Título revista:Helgoland Marine Research
Título revista abreviado:Helgol. Mar. Res.
ISSN:1438387X
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1438387X_v68_n4_p523_Saad

Referencias:

  • Akaike, H., A new look at the statistical model identifications (1974) IEEE Trans Autom Control, 19, pp. 716-723
  • Alexander, J., Valdés, A., The ring doesn’t mean a thing: molecular data suggest a new taxonomy for two Pacific species of sea hares (Mollusca: Opisthobranchia, Aplysiidae) (2013) Pac Sci, 67, pp. 283-294
  • Bleakney, J.S., Morphological variation in the radula of Placida dendritica (Alder and Hanrork, 1843) (Opisthobranchia: Ascoglossa/Sacoglossa) from Atlantic and Pacific populations (1989) Veliger, 32, pp. 171-181
  • Bleakney, J.S., Indirect evidence of a morphological response in the radula of Placida dendritica (Alder and Hancock, 1843) (Opisthobranchia: Ascoglossa/Sacoglossa) to different algal prey (1990) Veliger, 33, pp. 111-115
  • Clement, M., Posada, D., Crandall, K.A., TCS: a computer program to estimate gene genealogies (2000) Mol Ecol, 9, pp. 1657-1659. , COI: 1:CAS:528:DC%2BD3cXnvV2gtbw%3D, PID: 11050560
  • Colgan, D.J., McLauchlan, A., Wilson, G.D.F., Livingston, S.P., Edgecombe, G.D., Macaranas, J., Cassis, G., Gray, M.R., Histone H3 and U2 snRNA DNA sequences and arthropod molecular evolution (1998) Aust J Zool, 46, pp. 419-437
  • Drummond, A.J., Ashton, B., Cheung, M., Heled, J., Kearse, M., Moir, R., Stones-Havas, S., Wilson, A.C., (2009) Geneious ver, 4, p. 8. , http://www.geneious.com
  • Eales, N.B., Revision of the world species of Aplysia (Gastropoda Opisthobranchia) (1960) Bull Brit Mus (Nat Hist) Zool, 5, pp. 267-404
  • Excoffier, L., Lischer, H.E.L., Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows (2010) Mol Ecol Resour, 10, pp. 564-567. , PID: 21565059
  • Farris, J.S., Kallersjo, M., Kluge, A.G., Bult, C., Testing significance of incongruence (1994) Cladistics, 10, pp. 315-319
  • Folmer, O., Black, M., Hoeh, W., Lutz, R., Vrijenhoek, R., DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates (1994) Mol Mar Biol Biotechnol, 3, pp. 294-299. , COI: 1:CAS:528:DyaK2MXjt12gtLs%3D, PID: 7881515
  • Jensen, K.R., Morphological adaptations and plasticity of radular teeth of the Sacoglossa (= Ascoglossa) (Mollusca: Opisthobranchia) in relation to their food plants (1993) Biol J Linn Soc, 48, pp. 135-155
  • MacMunn, C.A., The pigments of Aplysia punctata (1899) J Physiol, 24, pp. 1-10. , COI: 1:CAS:528:DyaD28Xpsl2lsQ%3D%3D
  • Marcus, E., Marcus, E., Notes on Aplysia (1958) Univ São Paulo, Bull Inst Oceanogr, 8, pp. 3-22
  • Martínez, E., Ortea, J., On the synonymy between Aplysia winneba Eales, 1957 and Aplysia fasciata Poiret 1789 (Mollusca: Opisthobranchia: Anaspidea) (2002) Iberus, 20, pp. 11-21
  • Medina, M., Collins, T.M., Walsh, P.J., Phylogeny of sea hares in the Aplysia clade based on mitochondrial DNA sequence data (2005) Bull Mar Sci, 76, pp. 691-698
  • Medina, M., Collins, T.M., Walsh, P.J., mtDNA ribosomal gene phylogeny of sea hares in the genus Aplysia (Gastropoda, Opisthobranchia, Anaspidea): implications for comparative neurobiology (2001) Syst Biol, 50, pp. 676-688. , COI: 1:STN:280:DC%2BD38zntVKksA%3D%3D, PID: 12116938
  • Mickevich, M.F., Farris, J.S., The implications of congruence in Menidia (1981) Syst Zool, 30, pp. 351-370
  • http://www.abc.se/∼nylander, Nylander JAA (2004) MrModeltest ver.2. Evolutionary Biology Centre, Uppsala University, Uppsala; Ornelas-Gatdula, E., Dupont, A., Valdés, A., The tail tells the tale: taxonomy and biogeography of some Atlantic Chelidonura (Gastropoda: Cephalaspidea: Aglajidae) inferred from nuclear and mitochondrial gene data (2011) Zool J Linn Soc, 163, pp. 1077-1095
  • Palumbi, S.R., Nucleic acids II: the polymerase chain reaction (1996) Molecular systematics, pp. 205-247. , Hillis DM, Moritz C, Mable BK, (eds), Sinauer, Sunderland:
  • Pola, M., Vallès, Y., Cervera, J.L., Medina, M., Gosliner, T.M., Taxonomic status of Tambja abdere and Tambja fuscabased based on morphological and molecular evidence, with comments on the phylogeny of the subfamily Nembrothinae (Nudibranchia: Polyceridae) (2006) Ann Zool Fenn, 43, pp. 52-64
  • http://beast.bio.ed.ac.uk/Tracer, Rambaut A, Drummond AJ (2007) Tracer v1.5; http://sysbio.oxfordjournals.org/content/early/2012/03/02/sysbio.sys029.short, Ronquist F, Teslenko M, Van der Mark P, Ayres DL, Darling A, Hohna S, Larget B, Liu L, Suchard MA, Huelsenbeck J (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space; Silvestro, D., Michalak, I., Raxml GUI: a graphical front-end for RAxML (2012) Org Divers Evol, 12, pp. 335-337
  • Swofford, D.L., PAUP*: Phylogenetic analysis using parsimony (*and other methods). Ver. 4 (2002) Sinauer, , Sunderland, Massachusetts:
  • Valdés, A., Ornelas-Gatdula, E., Dupont, A., Color pattern variation in a shallow-water species of Opisthobranch Mollusc (2013) Biol Bull, 224, pp. 35-46. , PID: 23493507
  • Winkler, L.R., A mechanism of color variation operating in the west coast sea hare, Aplysia californica Cooper (1959) Pac Sci, 13, pp. 63-66

Citas:

---------- APA ----------
Saad, L.O., Cunha, C.M., Colpo, K.D. & Valdés, Á. (2014) . It’s not what it looks like: molecular data fails to substantiate morphological differences in two sea hares (Mollusca, Heterobranchia, Aplysiidae) from southern Brazil. Helgoland Marine Research, 68(4), 523-530.
http://dx.doi.org/10.1007/s10152-014-0408-1
---------- CHICAGO ----------
Saad, L.O., Cunha, C.M., Colpo, K.D., Valdés, Á. "It’s not what it looks like: molecular data fails to substantiate morphological differences in two sea hares (Mollusca, Heterobranchia, Aplysiidae) from southern Brazil" . Helgoland Marine Research 68, no. 4 (2014) : 523-530.
http://dx.doi.org/10.1007/s10152-014-0408-1
---------- MLA ----------
Saad, L.O., Cunha, C.M., Colpo, K.D., Valdés, Á. "It’s not what it looks like: molecular data fails to substantiate morphological differences in two sea hares (Mollusca, Heterobranchia, Aplysiidae) from southern Brazil" . Helgoland Marine Research, vol. 68, no. 4, 2014, pp. 523-530.
http://dx.doi.org/10.1007/s10152-014-0408-1
---------- VANCOUVER ----------
Saad, L.O., Cunha, C.M., Colpo, K.D., Valdés, Á. It’s not what it looks like: molecular data fails to substantiate morphological differences in two sea hares (Mollusca, Heterobranchia, Aplysiidae) from southern Brazil. Helgol. Mar. Res. 2014;68(4):523-530.
http://dx.doi.org/10.1007/s10152-014-0408-1