Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Purpose: The relative sensitivity of two freshwater invertebrate organisms to the organophosphorus insecticide fenitrothion was assessed by measuring cholinesterase (ChE) activity, a well-known biomarker of both exposure and effect to organophosphorus pesticides. The influence of different concentrations of humic acids (HAs) and particulate matter on fenitrothion bioavailability was assessed in the more sensitive species. Materials and methods: The selected invertebrates were the dwelling feeding oligochaete Lumbriculus variegatus and the pulmonate gastropod Biomphalaria glabrata. Acute 48-h bioassays were performed exposing organisms to different fenitrothion concentrations. The concentrations that induced 50 % inhibition of enzyme activity (EC50) were calculated. Fenitrothion bioavailability was investigated using different concentrations of commercial HA or particulate matter. Sand and a diverse selection of chromatographic resins that have been proposed as analogues of natural sediments were selected. For these experiments, animals were exposed to a fenitrothion value similar to the EC50. Results and discussion: The 48-h EC50 values were 12 ± 2 and 23 ± 3 μg l-1 for L. variegatus and B. glabrata, respectively. Depending on HA concentration and the characteristics of particles, ChE activity was similar or higher than the value recorded for animals exposed only to the pesticide in aqueous solution. Conclusion: The results indicated that L. variegatus was the more sensitive species of the two. In this species, fenitrothion bioavailability did not increase due to the presence of either different HA concentrations or particulate matter. The experimental approach may constitute a useful tool to predict the influence of dissolved organic matter and sediment particles on fenitrothion bioavailability and toxicity to non-target aquatic invertebrates. © 2013 Springer-Verlag Berlin Heidelberg.

Registro:

Documento: Artículo
Título:Combined effects of technical grade fenitrothion, humic acids and particulate matter on cholinesterase activity in freshwater invertebrates
Autor:Cánepa, A.; Basack, S.B.; Casabé, N.B.; Verrengia Guerrero, N.R.
Filiación:Toxicología y Química Legal, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Iquibicen-Conicet, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Bioavailability; Biomarkers; Biomphalaria glabrata; Lumbriculus variegatus; Organic matter; annelid; aqueous solution; bioassay; bioavailability; biomarker; chromatography; concentration (composition); enzyme activity; fenitrothion; freshwater ecosystem; humic acid; inhibition; macroinvertebrate; nontarget organism; organic matter; organophosphate; particulate matter; resin; sediment pollution; snail; toxicity; Animalia; Biomphalaria glabrata; Gastropoda; Invertebrata; Lumbriculus variegatus
Año:2013
Volumen:13
Número:4
Página de inicio:775
Página de fin:782
DOI: http://dx.doi.org/10.1007/s11368-012-0644-4
Título revista:Journal of Soils and Sediments
Título revista abreviado:J. Soils Sed.
ISSN:14390108
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14390108_v13_n4_p775_Canepa

Referencias:

  • Alves Costa, J.R.M., Mela, M., Silva de Assis, H.C., D'Pelletier, E., Randi, M.A.F., Oliveira Ribeiro, C.A., Enzymatic inhibition and morphological changes in Hoplias malabaricus from dietary exposure to lead (II) or methylmercury (2007) Ecotoxicol Environ Saf, 67, pp. 82-88
  • (2005) Standards Methods for the Examination of Water and Wastewater, , APHA-AWWA-WPCF21th Edition; American Public Health Associations, USA: American Water Works Associations and Water Pollution Control Federation (APHA-AWWA-WPCF)
  • (2010) Standard test methods for measuring the toxicity of sediment-associated contaminants with fresh water invertebrates (ASTM E1706-00), , ASTM American Society for Testing and Materials (ASTM), Annual Book of ASTM Standards Volume 11. 05, West Conshohocken, PA, USA
  • Chambers, J.E., Carr, R.L., Biochemical mechanisms contributing to species differences in insecticide toxicity (1995) Toxicology, 105, pp. 291-304
  • Conrad, A.U., Comber, S.D., Simkiss, K., New method for the assessment of contaminant uptake in the oligochaete Lumbriculus variegatus (2000) Bull Environ Contam Toxicol, 65, pp. 16-21
  • Davies, N.A., Edwards, P.A., Lawrence, M.A.M., Simkiss, K., Taylor, M.G., Biocide testing using particles with controlled surface properties (artificial sediments) (1999) Environ Toxicol Chem, 18, pp. 2337-2342
  • Devi, M., Fingerman, M., Inhibition of acethylcholinesterase activity in the central nervous system of the red swamp crayfish, Procambarus clarkii, by mercury, cadmium, and lead (1995) Bull Environ Contam Toxicol, 55, pp. 746-750
  • Di Rienzo, J.A., Casanoves, F., Balzarini, M.G., Gonzalez, L., Tablada, M., Robledo, C.W., (2008) Infostat, versión 2008, Grupo Infostat, FCA, , Argentina: Universidad Nacional de Córdoba
  • Eidt, D.C., Sosiak, A.J., Mallet, V.N., Partitioning and short-term persistence of fenitrothion in New Brunswick (Canada) headwater streams (1984) Arch Environ Contam Toxicol, 13, pp. 43-52
  • Ellman, G.L., Courtney, K.D., Jr, A.V., Featherstone, R.M., A new and rapid colorimetric determination of acetylcholinesterase activity (1961) Biochem Pharmacol, 7, pp. 88-95
  • Ferrari, A., Venturino, A., Pechen de D'Angelo, A.M., Time course of brain cholinesterase inhibition and recovery following acute and subacute azinphosmethyl, parathion and carbaryl exposure in the goldfish (Carassius auratus) (2004) Ecotoxicol Environ Saf, 57, pp. 420-425
  • Fried, B., Sundar Rao, K., Sherma, J., Fatty acid composition of Biomphalaria glabrata (Gastropoda: Planorbidae) fed hens egg yolk versus leaf lettuce (1992) Comp Biochem Physiol A, 101, pp. 351-352
  • Fulton, M.H., Key, P.B., Acetylcholinesterase inhibition in estuarine fish and invertebrates as indicator of organophosphorus insecticide exposure and effects (2001) Environ Toxicol Chem, 20, pp. 37-45
  • Giovanelli, A., Vieira, M.V., da Silva, C.L.P.A.C., Interaction between the intermediate host of Schistosomiasis in Brazil, Biomphalaria Glabrata (Say, 1818) and a possible competitor, Melanoides Tuberculata (Müller, 1774): a field study (2005) J Mollus Stud, 71, pp. 7-13
  • Gruber, S.I., Munn, M.D., Organophosphate and carbamate insecticides in agricultural waters and cholinesterase (ChE) inhibition in Common carp (Cyprinus carpio) (1998) Arch Environ Contam Toxicol, 35, pp. 391-396
  • Guilhermino, L., Lacerda, M.N., Nogueira, A.J.A., Soares, A.M.V.M., In vitro and in vivo inhibition of Daphnia magna acetylcholinesterase by surfactant agents: possible implications for contamination biomonitoring (2000) Sci Total Environ, 247, pp. 137-141
  • Haitzer, M., Höss, S., Traunspurger, W., Steinberg, C., Effects of dissolved organic matter (DOM) on the bioconcentration of organic chemicals in aquatic organisms-a review (1998) Chemosphere, 37, pp. 1335-1362
  • Koopal, L.K., van Riemsdijk, W.H., Kinniburgh, D.G., Humic matter and contaminants. General aspects and modeling metal ion binding (2001) Pure Appl Chem, 73, pp. 2005-2016
  • Kristoff, G., Verrengia Guerrero, N.R., Pechén de D'Angelo, A.M., Cochón, A.C., Inhibition of cholinesterase activity by azinphos-methyl in two freshwater invertebrates: Biomphalaria glabrata and Lumbriculus variegatus (2006) Toxicology, 222, pp. 185-194
  • Kristoff, G., Verrengia Guerrero, N.R., Cochón, A.C., Inhibition of cholinesterases and carboxylesterases of two invertebrate species, Biomphalaria glabrata and Lumbriculus variegatus, by the carbamate pesticide carbaryl (2010) Aquat Toxicol, 96, pp. 115-123
  • Láng, G., Kufcsák, O., Szegletes, T., Nemcsók, J., Quantitive distributions of different cholinesterases and inhibition of acetylcholinesterase by metidathion and paraquat in alimentary canal of common carp (1997) Gen Pharmac, 29, pp. 55-59
  • Lowry, O.H., Rosebrough, A., Farr, L., Randall, R.J., Protein measurement with the folin phenol reagent (1951) J Biol Chem, 193, pp. 265-275
  • Martinez-Tabche, L., Grajeday Ortega, M.A., Ramirez Mora, B., German Faz, C., Lopez Lopez, E., Galar Martinez, M., Hemoglobin concentration and acetylcholinesterase activity of oligochaetes in relation to lead concentration in spiked sediments from Ignacio Ramirez Reservoir (2001) Ecotoxicol Environ Saf, 49, pp. 76-83
  • Mount, D.R., Highland, T.L., Mattson, V.R., Dawson, Y.D., Lott, K.G., Ingersoll, C.G., Use of the oligochaete, Lumbriculus variegatus, as a prey organism for toxicant exposure of fish through the diet (2006) Environ Toxicol Chem, 25, pp. 2760-2767
  • (2007) Sediment-Water Lumbriculus Toxicity Test Using Spiked Sediment, , OECDGuidelines for the testing of chemicals, no. 225, Paris, France: Organization of Economic Cooperation and Development (OECD)
  • Payne, J.F., Mathieu, A., Melvin, W., Francey, L.L., Acetylcholinesterase, an old biomarker with a new future? Field trials in association with two urban rivers and a paper mill in Newfoundland (1996) Mar Pollut Bull, 32, pp. 225-231
  • Pointier, J.P., David, P., Jarne, P., Biological invasions: the case of planorbid snails (2005) J Helminthol, 79, pp. 249-256
  • Rand, G.M., Wells, P.G., McCarty, L.S., Introduction to aquatic toxicology (1995) Fundamentals of Aquatic Toxicology, pp. 3-67. , G. M. Rand (Ed.), London: Francis & Taylor
  • Sánchez-Hernández, J.C., Ecotoxicological perspectives of β-esterases in the assessment of pesticide contamination (2007) Environmental Pollution: New Research, pp. 1-45. , R. H. Plattenberg (Ed.), New York: Nova Science Publishers
  • Sancho, E., Ferrando, M.D., Andreu, E., In vivo inhibition of AChE activity in the European eel Anguilla anguilla exposed to technical grade fenitrothion (1998) Comp Biochem Physiol C, 120, pp. 389-395
  • Schmidt, G.H., Ibrahim, N.H.M., Heavy metal content (Hg2+, Cd2+, Pb2+) in various body parts: its impact on cholinesterase activity and binding glycoproteins in the grasshopper Aiolopus thalassimus adults (1994) Ecotoxicol Environ Saf, 29, pp. 148-164
  • (2010) Control of Priority and Dangerous Substances and Specific Pollutants in the Water Environment. EP061, , SEPA, Stirling: Scottish Environmental Protection Agency (SEPA)
  • Simkiss, K., Edwards, P.A., Lawrence, M.A.M., Davies, N.A., Taylor, M.G., The use of hydrophobic resins as analogues for sediment testing (2000) Environ Sci Technol, 34, pp. 2388-2392
  • Simkiss, K., Davies, N.A., Edwards, P.A., Lawrence, M.A.M., Taylor, M.G., The use of sediment analogues to study the uptake of pollutants by chironomid larvae (2001) Environ Pollut, 115, pp. 89-96
  • Szabó, A., Nemcsók, J., Asztalos, B., Rakonczay, Z., Kása, P., Huu Hieu, L., The effect of pesticides on carp (Cyprinus carpio L.). Acetylcholinesterase and its biochemical characterization (1992) Ecotoxicol Environ Saf, 23, pp. 39-45
  • (2000) Methods for measuring the toxicity and bioaccumulation of sediment-associated contaminants with freshwater invertebrates, , USEPA United States Environmental Protection Agency (USEPA), Washington DC, USA
  • Verrengia Guerrero, N.R., Predicting the uptake and bioaccumulation of organic pollutants from natural sediments (2007) Environmental Pollution: New Research, pp. 141-184. , R. H. Plattenberg (Ed.), New York, USA: Nova Science Publishers
  • Whitehead, A., Anderson, S.L., Ramirez, A., Wilson, B.W., Cholinesterases in aquatic biomonitoring: assay optimization and species-specific characterization for a California native fish (2005) Ecotoxicology, 14, pp. 597-606
  • (1991) Fenitrothion: Health and Safety Guide, No. 65, , WHO, Geneva, Switzerland: World Health Organization (WHO)

Citas:

---------- APA ----------
Cánepa, A., Basack, S.B., Casabé, N.B. & Verrengia Guerrero, N.R. (2013) . Combined effects of technical grade fenitrothion, humic acids and particulate matter on cholinesterase activity in freshwater invertebrates. Journal of Soils and Sediments, 13(4), 775-782.
http://dx.doi.org/10.1007/s11368-012-0644-4
---------- CHICAGO ----------
Cánepa, A., Basack, S.B., Casabé, N.B., Verrengia Guerrero, N.R. "Combined effects of technical grade fenitrothion, humic acids and particulate matter on cholinesterase activity in freshwater invertebrates" . Journal of Soils and Sediments 13, no. 4 (2013) : 775-782.
http://dx.doi.org/10.1007/s11368-012-0644-4
---------- MLA ----------
Cánepa, A., Basack, S.B., Casabé, N.B., Verrengia Guerrero, N.R. "Combined effects of technical grade fenitrothion, humic acids and particulate matter on cholinesterase activity in freshwater invertebrates" . Journal of Soils and Sediments, vol. 13, no. 4, 2013, pp. 775-782.
http://dx.doi.org/10.1007/s11368-012-0644-4
---------- VANCOUVER ----------
Cánepa, A., Basack, S.B., Casabé, N.B., Verrengia Guerrero, N.R. Combined effects of technical grade fenitrothion, humic acids and particulate matter on cholinesterase activity in freshwater invertebrates. J. Soils Sed. 2013;13(4):775-782.
http://dx.doi.org/10.1007/s11368-012-0644-4