Artículo

Bracalente, C.; Ibañez, I.L.; Molinari, B.; Palmieri, M.A.; Maglioco, A.; Policastro, L.; Kreiner, A.J.; Burlón, A.; Valda, A.; Davidson, J.; Davidson, M.; Vázquez, M.; Ozafrán, M.; Durán, H. "Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation" (2010) International Journal of Low Radiation. 7(5):393-408
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Abstract:

The aim of this study was to evaluate the DNA damage induced by low and high linear energy transfer radiation in two related cell lines with different radiation sensitivity, CHO10B2 and irs-20 cells (defective in DNAPKcs). Double-strand breaks were assessed by the detection of phosphorylated histone H2AX foci (γH2AX). The number of foci increased as a function of dose for both cell lines and types of radiation. However, irs-20 cells showed a higher number of foci than the parental CHO10B2. An increase in the foci size was observed for both cell lines after lithium irradiation. This could be attributed to clusters of DNA damage. Moreover, the number of lithiuminduced larger foci per nuclei increased with dose and fitted with the expected number of hits per nucleus. Concluding, γH2AX foci size provides a potential tool to characterise the intrinsic radiosensitivity of cells related to DNA damage and to compare the effects of different quality radiations. Copyright © 2010 Inderscience Enterprises Ltd.

Registro:

Documento: Artículo
Título:Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation
Autor:Bracalente, C.; Ibañez, I.L.; Molinari, B.; Palmieri, M.A.; Maglioco, A.; Policastro, L.; Kreiner, A.J.; Burlón, A.; Valda, A.; Davidson, J.; Davidson, M.; Vázquez, M.; Ozafrán, M.; Durán, H.
Filiación:Comisión Nacional de Energía Atómica, Av. Gral. Paz 1499, (1650) San Martín, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas, Avda. Rivadavia 1917, Cdad. de Buenos Aires, Argentina
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín, Argentina
Palabras clave:γH2AX; CHO10B2; DNA double strand break; DNA-PKcs; H2AX foci size; Ionising radiation; irs-20; LET; Linear energy transfer; histone gamma h2ax; histone H2AX; lithium; unclassified drug; animal cell; article; controlled study; DNA damage; double stranded DNA break; gamma irradiation; immunofluorescence; ionizing radiation; linear energy transfer; nonhuman; protein phosphorylation; radiation dose fractionation; radiosensitivity
Año:2010
Volumen:7
Número:5
Página de inicio:393
Página de fin:408
DOI: http://dx.doi.org/10.1504/IJLR.2010.036968
Título revista:International Journal of Low Radiation
Título revista abreviado:Int. J. Low Radiat.
ISSN:14776545
CAS:lithium, 7439-93-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14776545_v7_n5_p393_Bracalente

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Citas:

---------- APA ----------
Bracalente, C., Ibañez, I.L., Molinari, B., Palmieri, M.A., Maglioco, A., Policastro, L., Kreiner, A.J.,..., Durán, H. (2010) . Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation. International Journal of Low Radiation, 7(5), 393-408.
http://dx.doi.org/10.1504/IJLR.2010.036968
---------- CHICAGO ----------
Bracalente, C., Ibañez, I.L., Molinari, B., Palmieri, M.A., Maglioco, A., Policastro, L., et al. "Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation" . International Journal of Low Radiation 7, no. 5 (2010) : 393-408.
http://dx.doi.org/10.1504/IJLR.2010.036968
---------- MLA ----------
Bracalente, C., Ibañez, I.L., Molinari, B., Palmieri, M.A., Maglioco, A., Policastro, L., et al. "Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation" . International Journal of Low Radiation, vol. 7, no. 5, 2010, pp. 393-408.
http://dx.doi.org/10.1504/IJLR.2010.036968
---------- VANCOUVER ----------
Bracalente, C., Ibañez, I.L., Molinari, B., Palmieri, M.A., Maglioco, A., Policastro, L., et al. Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation. Int. J. Low Radiat. 2010;7(5):393-408.
http://dx.doi.org/10.1504/IJLR.2010.036968