Silveyra, G.R.; Canosa, I.S.; Rodríguez, E.M.; Medesani, D.A. "Effects of atrazine on ovarian growth, in the estuarine crab Neohelice granulata" (2017) Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology. 192:1-6
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor


Atrazine, a herbicide that is intensively used in Argentina, was assayed to evaluate the alteration of reproduction in a wild species of crustaceans. Adult females of the estuarine crab Neohelice granulata were exposed to formulated atrazine during the 3-month pre-reproductive period. Three atrazine concentrations (0.03, 0.3 and 3 mg/L) were assayed, together with a water dilution control. At the end of the exposure period, several variables concerning the energetic status of animals were measured, such as weight gain, glycemia, and energy reserves in both muscle and hepatopancreas. The gonadosomatic index (GSI) was also determined, as well as the proportion and relative area of each oocyte type in histological sections. Besides, the total content of vitellogenin proteins (Vg) in both ovary and hepatopancreas was measured. A significant (p < 0.05) decrease of glycogen content was observed in muscle, while a significant (p < 0.05) lower area of both previtellogenic and vitellogenic oocytes was verified in the ovary by effect of atrazine, in correspondence with a Vg content significantly (p < 0.05) diminished in the ovary and augmented in the hepatopancreas. Besides, a higher proportion of previtellogenic oocytes were seen by effect of atrazine. Taken together, these results indicate a clear reduction and delay in the ovarian growth of the studied species, during the period at which the ovary normally grows up prior to spawning. On the other hand, a decrease of Vg content was observed at 0.3 and 3 mg/L of atrazine after 24-h in vitro assays carried out with ovarian explants, providing additional evidence about the inhibitory role of this herbicide on the ovarian growth. These results encourage future research on deleterious effects of atrazine on crustacean reproduction. © 2016 Elsevier Inc.


Documento: Artículo
Título:Effects of atrazine on ovarian growth, in the estuarine crab Neohelice granulata
Autor:Silveyra, G.R.; Canosa, I.S.; Rodríguez, E.M.; Medesani, D.A.
Filiación:Dept. of Biodiversity and Experimental Biology, FCEN, University of Buenos Aires, Institute of Biodiversity, Experimental and Applied Biology (IBBEA), CONICET-UBA, Ciudad Universitaria, Pab. II, Buenos Aires, C1428EGA, Argentina
Palabras clave:Atrazine; Crabs; Ovary; Reproduction; Vitellogenin; atrazine; glycogen; vitellogenin; atrazine; glycogen; herbicide; vitellogenin; water pollutant; adult; animal experiment; animal tissue; Argentina; Article; concentration (parameters); controlled study; crab; energy resource; energy transfer; estuarine species; female; glucose blood level; gonadosomatic index; hepatopancreas; histology; in vitro study; molting; mortality; muscle; nonhuman; oocyte; ovary development; parameters; priority journal; reproductive toxicity; spawning; survival; weight gain; animal; Brachyura; dose response; drug effects; energy metabolism; growth, development and aging; metabolism; ovary; reproduction; skeletal muscle; time factor; tissue culture technique; toxicity; water pollutant; Animals; Atrazine; Brachyura; Dose-Response Relationship, Drug; Energy Metabolism; Female; Glycogen; Hepatopancreas; Herbicides; Muscle, Skeletal; Oocytes; Ovary; Reproduction; Time Factors; Tissue Culture Techniques; Vitellogenins; Water Pollutants, Chemical
Página de inicio:1
Página de fin:6
Título revista:Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Título revista abreviado:Comp. Biochem. Physiol. C Toxicol. Pharmacol.
CAS:atrazine, 1912-24-9; glycogen, 9005-79-2; Atrazine; Glycogen; Herbicides; Vitellogenins; Water Pollutants, Chemical


  • Álvarez, N.B., Avigliano, L., Mac Loughlin, C., Rodríguez, E.M., The adverse effect of the herbicide atrazine on the reproduction in the intertidal varunid crab Neohelice granulata (Dana, 1851) (2015) Reg. Stud. Mar. Sci., 1, pp. 1-6
  • American Public Health Association, American Water Works Association, Water Pollution Control Federation, Standard Methods for the Examination of Water and Wastewaters (2005), 21th ed. American Public Health Association Washington DC; Arancibia, F., Challenging the bioeconomy: the dynamics of collective action in Argentina (2013) Technol. Soc., 35, pp. 79-92
  • Atanor, Agroquímicos: Atrazina (2012),; Charmantier, G., Charmantier-Daures, M., Van Herp, F., Hormonal regulation of growth and reproduction in crustaceans (1997) Recent Advances in Marine Biotechnology, I, pp. 109-161. , M. Fingerman R. Nagabhushanam M.F. Thompson Oxford and IBH Publishing New Delhi
  • Chaulet, A., Medesani, D.A., Freitas, J., Cervino, A., Cervino, N., Rodríguez, E.M., Induction of somatic growth in juvenile crayfish Cherax quadricarinatus (Decapoda, Parastacidae), by ecdysone and insulin growth factor (2012) Aquaculture, 370-371, pp. 1-6
  • Comisión Administradora del Río de la Plata, Estudio para la evaluación de la contaminación en el Río de la Plata (1990), Argentine Hydrographic Service Navy Buenos Aires, Argentina; Cooke, I.M., Haylett, B.A., Weatherby, T.M., Electrically elicited neurosecretory and electrical responses of the isolated crab sinus gland in normal and reduced calcium salines (1977) J. Exp. Biol., 101, pp. 125-149
  • De Geronimo, E., Aparicio, V.C., Bárbaro, S., Portocarrero, R., Jaime, S., Costa, J.L., Presence of pesticides in surface water from four sub-basins in Argentina (2014) Chemosphere, 107, pp. 423-431
  • Dodson, S., Merritt, C., Shannahan, J., Shults, C., Low exposure concentrations of atrazine increase male production in Daphnia pulicaria (1999) Environ. Toxicol. Chem., 18, pp. 1568-1573
  • Dos Santos, K.C., Martinez, C.B.R., Genotoxic and biochemical effects of atrazine and Roundup®, alone and in combination, on the Asian clam Corbicula fluminea (2014) Ecotoxicol. Environ. Saf., 100, pp. 7-14
  • Dreon, M.S., Heras, H., Pollero, R.J., Metabolism of ovorubin, the major egg lipoprotein from the apple snail (2003) Mol. Cell. Biochem., 243, pp. 9-14
  • Fingerman, M., Roles of neurotransmitters in regulating reproductive hormone release and gonadal maturation in decapod crustaceans (1997) Invertebr. Reprod. Dev., 31, pp. 47-54
  • Folch, J., Lees, M., Stanley, G.H.S., A simple method for isolation and purification of total lipids from animal tissues (1957) J. Biol. Chem., 226, pp. 497-509
  • Fring, C.S., Dunn, R.T., A colorimetric method for determination of total serum lipids based on the sulfophosphovanilina reaction (1970) Am. J. Clin. Pathol., 53, pp. 89-91
  • García, F., Cunningham, M.L., Garda, H., Heras, H., Embryo lipoproteins and yolk lipovitellin consumption during embryogenesis in Macrobrachium borellii (Crustacea: Palaemonidae) (2008) Comp. Biochem. Physiol., 151B, pp. 317-322
  • Graymore, M., Stagnitti, F., Allinson, G., Impacts of atrazine in aquatic ecosystems (2001) Environ. Int., 26, pp. 483-495
  • Jablonowski, N.D., Schäffer, A., Burauel, P., Still present after all these years: persistence plus potential toxicity raise questions about the use of atrazine (2011) Environ. Sci. Pollut. Res., 18, pp. 328-331
  • Lascombe, I., Beffa, D., Ruegg, U., Tarradellas, J., Wahli, W., Estrogenic activity assessment of environmental chemicals using in vitro assays: identification of two new estrogenic compounds (2000) Environ. Health Perspect., 108, pp. 621-629
  • Laufer, H., Demir, N., Pan, X., Stuart, J.D., Ahl, J.S.B., Methyl farnesoate controls adult male morphogenesis in the crayfish, Procambarus clarkii (2005) J. Insect Physiol., 51, pp. 379-384
  • López Greco, L.S., Rodríguez, E.M., Annual reproduction and growth of adult crabs, Chasmagnathus granulata (Crustacea, Brachyura, Grapsidae) (1999) Cah. Biol. Mar., 40, pp. 155-164
  • Lowry, O.H., Rosenbrough, N.J., Randall, R.J., Protein measurements with the Folin phenol reagent (1951) J. Biol. Chem., 183, pp. 265-275
  • McKinlay, R., Plant, J.A., Bell, J., Voulvoulis, N., Endocrine disrupting pesticides: implications for risk assessment (2008) Environ. Int., 34, pp. 168-183
  • Medesani, D.A., López Greco, L.S., Rodríguez, E.M., Interference of cadmium and copper with the endocrine control of ovarian growth, in the estuarine crab Chasmagnathus granulata (2004) Aquat. Toxicol., 69, pp. 165-174
  • Medesani, D.A., Ferré, L.E., Grodzielski, M., Rodríguez, E.M., In vitro effect of methyl farnesoate on the vitellogenin content of ovary and hepatopancreas, in the crayfish Cherax quadricarinatus (2012) Invertebr. Reprod. Dev., 56, pp. 138-143
  • Meunpol, O., Iam-Pai, S., Suthikrai, W., Piyatiratitivorakul, S., Identification of progesterone and 17α-hydroxyprogesterone in polychaetes (Perinereis sp.) and the effects of hormone extracts on penaeid oocyte development in vitro (2007) Aquaculture, 270, pp. 485-492
  • Nagaraju, G.P.C., Is methyl farnesoate a crustacean hormone? (2007) Aquaculture, 272, pp. 39-54
  • Nagaraju, G.P.C., Reproductive regulators in decapod crustaceans: an overview (2011) J. Exp. Biol., 214, pp. 3-16
  • Palma, P., Palma, V.L., Matos, C., Fernandes, R.M., Bohn, A., Soares, A.M.V.M., Barbosa, I., Effects of atrazine and endosulfan sulphate on the ecdysteroid system of Daphnia magna (2009) Chemosphere, 74, pp. 676-681
  • Rodríguez, E.M., Schuldt, M., Romano, L., Chronic histopathological effects of parathion and 2,4-D on female gonads of Chasmagnathus granulata (Decapoda, Brachyura) (1994) Food Chem. Toxicol., 32, pp. 811-818
  • Rodríguez, E.M., López Greco, L.S., Fingerman, M., Inhibition of ovarian growth by cadmium in the fiddler crab, Uca pugilator (Decapoda, Ocypodidae) (2000) Ecotoxicol. Environ. Saf., 46, pp. 202-206
  • Sánchez, F., Mari, N., Lasta, C., Giangioble, A., Alimentación de la corvina rubia (Micropogonias furnieri) en la Bahía Samborombon (1991) Frente Marítimo, 8, pp. 43-50
  • Sarojini, R., Nagabhushanam, R., Fingerman, M., An in vitro study of the inhibitory action of methionine enkephalin on ovarian maturation in the red swamp crayfish, Procambarus clarkii (1997) Comp. Biochem. Physiol., 117C, pp. 207-210
  • Sokal, R.R., Rohlf, F.J., Biometry (1981), second ed. Freeman New York; Sokolova, I.M., Frederich, M., Bagwe, R., Lannig, G., Sukhotin, A.A., Energy homeostasis as an integrative tool for assessing limits of environmental stress tolerance in aquatic invertebrates (2012) Mar. Environ. Res., 79, pp. 1-15
  • Tillitt, D.E., Papoulias, D.M., Whyte, J.J., Richter, C.A., Atrazine reduces reproduction in fathead minnow (Pimephales promelas) (2010) Aquat. Toxicol., 99, pp. 149-159
  • USEPA (United States Environmental Protection Agency), Reregistration Eligibility Science Chapter for Atrazine Environmental Fate and Effects Chapter (2002), (Washington, DC, USA); Van Handel, E., Estimation of glycogen in small amount soft tissue (1965) Anal. Biochem., 11, pp. 256-265
  • Villeneuve, D.L., Blankenship, A.L., Giesy, J.P., Interactions between environmental xenobiotics and estrogen-mediated responses (1998) Toxicant-receptor Interactions, pp. 69-99. , M.S. Denison W.G. Helferich Taylor and Francis Philadelphia
  • Vonberg, D., Vanderborght, J., Cremer, N., Pütz, T., Herbst, M., Vereecken, H., 20 years of long-term atrazine monitoring in a shallow aquifer in western Germany (2014) Water Res., 50, pp. 294-306
  • Warrier, S.R., Tirumalai, R., Subramonian, T., Occurence of vertebrate steroids, estradiol 17β and progesterone in the reprodcing females of the mud crab Scylla serrata (2001) Comp. Biochem. Physiol., 130A, pp. 283-294


---------- APA ----------
Silveyra, G.R., Canosa, I.S., Rodríguez, E.M. & Medesani, D.A. (2017) . Effects of atrazine on ovarian growth, in the estuarine crab Neohelice granulata. Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 192, 1-6.
---------- CHICAGO ----------
Silveyra, G.R., Canosa, I.S., Rodríguez, E.M., Medesani, D.A. "Effects of atrazine on ovarian growth, in the estuarine crab Neohelice granulata" . Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology 192 (2017) : 1-6.
---------- MLA ----------
Silveyra, G.R., Canosa, I.S., Rodríguez, E.M., Medesani, D.A. "Effects of atrazine on ovarian growth, in the estuarine crab Neohelice granulata" . Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, vol. 192, 2017, pp. 1-6.
---------- VANCOUVER ----------
Silveyra, G.R., Canosa, I.S., Rodríguez, E.M., Medesani, D.A. Effects of atrazine on ovarian growth, in the estuarine crab Neohelice granulata. Comp. Biochem. Physiol. C Toxicol. Pharmacol. 2017;192:1-6.