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Abstract:

During early placentation, matrix metalloproteinases (MMPs) play important roles in decidualization, trophoblast migration, invasion, angiogenesis, vascularization and extracellular matrix (ECM) remodeling of the endometrium. The aim of our study was to analyze the localization, distribution and differential expression of MMP-2 and -9 in the organogenic implantation site and to evaluate in vivo and in vitro decidual MMP-2 and -9 activities on day 10 of gestation in CF-1 mouse. Whole extracts for Western blotting of organogenic E10-decidua expressed MMP-2 and -9 isoforms. MMP-2 immunoreactivity was found in a granular and discrete pattern in ECM of mesometrial decidua (MD) near maternal blood vessels and slightly in non-decidualized endometrium (NDE). Immunoexpression of MMP-9 was also detected in NDE, in cytoplasm of decidual cells and ECM of vascular MD, in trophoblastic area and in growing antimesometrial deciduum. Gelatin zymography showed that MMP-9 activity was significantly lower in CM compared to the active form of direct (not cultured) and cultured decidua. The decidual active MMP-9 was significantly higher than the active MMP-2. These results show differential localization, protein expression and enzymatic activation of MMPs, suggesting specific roles for MMP-2 and MMP-9 in decidual and trophoblast tissues related to organogenic ECM remodeling and vascularization during early establishment of mouse placentation. © Springer Science+Business Media B.V. 2012.

Registro:

Documento: Artículo
Título:Matrix metalloproteinase expression and activity in trophoblastdecidual tissues at organogenesis in CF-1 mouse
Autor:Fontana, V.; Coll, T.A.; Sobarzo, C.M.A.; Tito, L.P.; Calvo, J.C.; Cebral, E.
Filiación:Laboratorio de Química de Proteoglicanos y Matriz Extracelular, Depto. de Química Biológica (QB), Universidad de Buenos Aires (UBA), Buenos Aires, Argentina
Laboratorio de Reproducción y Fisiopatología Materno-Embrionaria (LARFIMAE), Depto. de Biodiversidad y Biología Experimental (DBBE), Intendente Güiraldes 2620, Ciudad Universitaria, Pabellón 2, Buenos Aires, Argentina
Facultad de Medicina, Instituto de Investigaciones Biomédicas(INBIOMED), Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Biología y Medicina Experimental, CONICET, Buenos Aires, Argentina
Palabras clave:CF-1 mouse; Decidua; Matrix metalloproteinases; Organogenesis; gelatinase A; gelatinase B; matrix metalloproteinase; animal tissue; article; controlled study; culture medium; cytoplasm; decidua; embryo; endometrium; enzyme activation; enzyme activity; extracellular matrix; female; in vitro study; in vivo study; mouse; nonhuman; organogenesis; priority journal; protein expression; protein localization; trophoblast; Western blotting; Animals; Decidua; Embryo Implantation; Endometrium; Extracellular Matrix; Female; Gene Expression Regulation, Developmental; Matrix Metalloproteinase 2; Matrix Metalloproteinase 9; Mice; Organogenesis; Placentation; Pregnancy; Trophoblasts
Año:2012
Volumen:43
Número:5
Página de inicio:487
Página de fin:496
DOI: http://dx.doi.org/10.1007/s10735-012-9429-8
Título revista:Journal of Molecular Histology
Título revista abreviado:J. Mol. Histol.
ISSN:15672379
CODEN:JMHOA
CAS:gelatinase A, 146480-35-5; gelatinase B, 146480-36-6; Matrix Metalloproteinase 2, 3.4.24.24; Matrix Metalloproteinase 9, 3.4.24.35
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15672379_v43_n5_p487_Fontana

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Citas:

---------- APA ----------
Fontana, V., Coll, T.A., Sobarzo, C.M.A., Tito, L.P., Calvo, J.C. & Cebral, E. (2012) . Matrix metalloproteinase expression and activity in trophoblastdecidual tissues at organogenesis in CF-1 mouse. Journal of Molecular Histology, 43(5), 487-496.
http://dx.doi.org/10.1007/s10735-012-9429-8
---------- CHICAGO ----------
Fontana, V., Coll, T.A., Sobarzo, C.M.A., Tito, L.P., Calvo, J.C., Cebral, E. "Matrix metalloproteinase expression and activity in trophoblastdecidual tissues at organogenesis in CF-1 mouse" . Journal of Molecular Histology 43, no. 5 (2012) : 487-496.
http://dx.doi.org/10.1007/s10735-012-9429-8
---------- MLA ----------
Fontana, V., Coll, T.A., Sobarzo, C.M.A., Tito, L.P., Calvo, J.C., Cebral, E. "Matrix metalloproteinase expression and activity in trophoblastdecidual tissues at organogenesis in CF-1 mouse" . Journal of Molecular Histology, vol. 43, no. 5, 2012, pp. 487-496.
http://dx.doi.org/10.1007/s10735-012-9429-8
---------- VANCOUVER ----------
Fontana, V., Coll, T.A., Sobarzo, C.M.A., Tito, L.P., Calvo, J.C., Cebral, E. Matrix metalloproteinase expression and activity in trophoblastdecidual tissues at organogenesis in CF-1 mouse. J. Mol. Histol. 2012;43(5):487-496.
http://dx.doi.org/10.1007/s10735-012-9429-8