Abstract:
We have developed a spatial light modulator (SLM) based microscope that uses diffraction to shape the incoming two-photon laser source to any arbitrary light pattern. This allows the simultaneous imaging or photostimulation of different regions of a sample with three-dimensional precision at high frame rates. Additionally, we have combined this microscope with a new class of two photon active neuromodulators with Ruthenium BiPyridine (RuBi) based cages that offer great flexibility for neuronal control. © 2010 Copyright SPIE - The International Society for Optical Engineering.
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Documento: |
Conferencia
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Título: | Fast two-photon neuronal imaging and control using a spatial light modulator and ruthenium compounds |
Autor: | Peterka, D.S.; Nikolenko, V.; Fino, E.; Araya, R.; Etchenique, R.; Yuste, R. |
Ciudad: | San Francisco, CA |
Filiación: | Dept. of Biological Sciences, Howard Hughes Medical Institute, Columbia University, New York, NY 10027, United States Departamento de Química Inorgánica, Analítica Y Química Física, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, C1428EHA, Argentina
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Palabras clave: | adaptive optics; imaging; microscope; neuron; Ruthenium; Spatial light modulator; two-photon; uncaging; Dimensional precision; High frame rate; imaging; Light patterns; Neuromodulators; Neuronal control; Photostimulation; Ruthenium bipyridine; Simultaneous imaging; Spatial light modulators; Two photon; uncaging; Light; Light modulation; Microscopes; Photons; Ruthenium; Ruthenium compounds; Transition metal compounds; Light modulators |
Año: | 2010
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Volumen: | 7548
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DOI: |
http://dx.doi.org/10.1117/12.842606 |
Título revista: | Photonic Therapeutics and Diagnostics VI
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Título revista abreviado: | Progr. Biomed. Opt. Imaging Proc. SPIE
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ISSN: | 16057422
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16057422_v7548_n_p_Peterka |
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Citas:
---------- APA ----------
Peterka, D.S., Nikolenko, V., Fino, E., Araya, R., Etchenique, R. & Yuste, R.
(2010)
. Fast two-photon neuronal imaging and control using a spatial light modulator and ruthenium compounds. Photonic Therapeutics and Diagnostics VI, 7548.
http://dx.doi.org/10.1117/12.842606---------- CHICAGO ----------
Peterka, D.S., Nikolenko, V., Fino, E., Araya, R., Etchenique, R., Yuste, R.
"Fast two-photon neuronal imaging and control using a spatial light modulator and ruthenium compounds"
. Photonic Therapeutics and Diagnostics VI 7548
(2010).
http://dx.doi.org/10.1117/12.842606---------- MLA ----------
Peterka, D.S., Nikolenko, V., Fino, E., Araya, R., Etchenique, R., Yuste, R.
"Fast two-photon neuronal imaging and control using a spatial light modulator and ruthenium compounds"
. Photonic Therapeutics and Diagnostics VI, vol. 7548, 2010.
http://dx.doi.org/10.1117/12.842606---------- VANCOUVER ----------
Peterka, D.S., Nikolenko, V., Fino, E., Araya, R., Etchenique, R., Yuste, R. Fast two-photon neuronal imaging and control using a spatial light modulator and ruthenium compounds. Progr. Biomed. Opt. Imaging Proc. SPIE. 2010;7548.
http://dx.doi.org/10.1117/12.842606