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Abstract:

The activity of Cinnamomum zeylanicum Nees essential oil (cinnamon oil) against Paenibacillus larvae (Withe) was evaluated in the laboratory and in a field experiment in order to improve the biological control of the Apis mellifera L. disease American foulbrood (AFB). The MICs (Minimal Inhibitory Concentration) against P. larvae were determined by the tube dilution method. Bee lethality was estimated using cages with approximately 374 bees, fed with the essential oil at different concentrations diluted in ethanolic syrup. The security index was calculated as LD50 of A. melliferaMIC of P. larvae strain at 24, 48 and 72 h in both tests. The apiary trial, using three groups of five hives each, was carried out in April-May 2006 in an experimental apiary of J. J. Nágera coastal station (Mar del Plata, Argentine). The first group was treated with cinnamon oil (two doses of 1000 ug/ml per hive), the second with oxytracycline-HCl (three doses of 0.4 g per hive) and the third was left untreated as a control. All of the treatments were performed at 7-day intervals. The evaluation of treatment efficacy was made by counting the number of infected brood cells in both sides of a central comb, using a brood surface of 360 cm2 (18 x 20 cm). For the Mar del Plata AFB strain the C. zeylanicum essential oil and antibiotic MICs were 50 μg/ml and 3.125 μg/ml, respectively. Regarding the systemic administration method, bees LD50 at 24 h was 456.07 μg of essential oil of cinnamon/bee. LD50 estimated at 48 h showed a slight decrease with respect to that recorded at 24 h. Security index was 9.1214 (ml/bee) for 24 h. In relation with field trials, after 24 and 31 days from the beginning of treatments the cinnamon oil-treated hives showed a lesser incidence of infected larvae (7.89% and 52.42%) than the control group highlighting a clear efficient control. No significant differences between the two treated groups were recorded. These results represent a further proof of the potential of cinnamon oil to control American Foulbrood with only minor toxicological risks to bees. Furthermore, the use of cinnamon oil avoids problems with antibiotic residues in honey. This is important in marketing honey in the EU where antibiotics in honey are generally forbidden by law.

Registro:

Documento: Artículo
Título:Advances in the apiary control of the honeybee american foulbrood with cinnamon (Cinnamomum zeylanicum) essential oil
Autor:Gende, L.B.; Maggi, M.D.; Damiani, N.; Fritz, R.; Eguaras, M.J.; Floris, I.
Filiación:Departamento de Biología, Facultad Ciencias Exactas y Naturales, Universidad Nacional de Mar Del Plata, Argentina
CONICET, Universidad Nacional de Mar Del Plata, Argentina
Dipartimento Protezione Piante, Sezione Entomologia, Università Degli Studi di Sassari, via E. De Nicola, 07100 Sassari, Italy
Universidad Nacional de Mar Del Plata, Funes 3350 (7600), Mar del Plata, Buenos Aires, Argentina
Idioma: Inglés
Palabras clave:American foulbrood control; Antimicrobial activity; Apiary trial; Cinnamon oil; Oxytetracycline; Apis mellifera; Apoidea; Cinnamomum verum; Paenibacillus; Paenibacillus larvae
Año:2009
Volumen:62
Número:1
Página de inicio:93
Página de fin:97
Título revista:Bulletin of Insectology
Título revista abreviado:Bull. Insect.
ISSN:17218861
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17218861_v62_n1_p93_Gende

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Citas:

---------- APA ----------
Gende, L.B., Maggi, M.D., Damiani, N., Fritz, R., Eguaras, M.J. & Floris, I. (2009) . Advances in the apiary control of the honeybee american foulbrood with cinnamon (Cinnamomum zeylanicum) essential oil. Bulletin of Insectology, 62(1), 93-97.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17218861_v62_n1_p93_Gende [ ]
---------- CHICAGO ----------
Gende, L.B., Maggi, M.D., Damiani, N., Fritz, R., Eguaras, M.J., Floris, I. "Advances in the apiary control of the honeybee american foulbrood with cinnamon (Cinnamomum zeylanicum) essential oil" . Bulletin of Insectology 62, no. 1 (2009) : 93-97.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17218861_v62_n1_p93_Gende [ ]
---------- MLA ----------
Gende, L.B., Maggi, M.D., Damiani, N., Fritz, R., Eguaras, M.J., Floris, I. "Advances in the apiary control of the honeybee american foulbrood with cinnamon (Cinnamomum zeylanicum) essential oil" . Bulletin of Insectology, vol. 62, no. 1, 2009, pp. 93-97.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17218861_v62_n1_p93_Gende [ ]
---------- VANCOUVER ----------
Gende, L.B., Maggi, M.D., Damiani, N., Fritz, R., Eguaras, M.J., Floris, I. Advances in the apiary control of the honeybee american foulbrood with cinnamon (Cinnamomum zeylanicum) essential oil. Bull. Insect. 2009;62(1):93-97.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17218861_v62_n1_p93_Gende [ ]