Artículo

Bonfiglio, J.J.; Maccarrone, G.; Rewerts, C.; Holsboer, F.; Arzt, E.; Turck, C.W.; Silberstein, S. "Characterization of the B-Raf interactome in mouse hippocampal neuronal cells" (2011) Journal of Proteomics. 74(2):186-198
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Abstract:

B-Raf links a variety of extracellular stimuli downstream of cell surface receptors, constituting a determining factor in the ability of neurons to activate ERK. A detailed study of the B-Raf interactome is necessary to clarify the intricacy of B-Raf-dependent signal transduction. We used a mouse hippocampal cell line (HT22) that expresses B-Raf at high levels, to identify B-Raf associated proteins under endogenous expression conditions, avoiding artificial interactions from overexpression studies. We used stringent procedures to co-immunoprecipitate proteins that specifically associate with endogenous B-Raf with the help of gel electrophoresis separation and off-line LC-MALDI-MS/MS proteomic analysis. Our stringent protein identification criteria allowed confident identification of B-Raf interacting proteins under non-stimulating conditions. The presence of previously reported B-Raf interactors among the list of proteins identified confirms the quality of proteomic data. We identified tubulin and actin as B-Raf interactors for the first time, among structural and accessory proteins of cell cytoskeleton, molecular chaperones (Hsc70, GRP78), and cellular components involved in aspects of mRNA metabolism and translation. Interactions were validated in HT22 cells and in the neuronal cell line Neuro-2a providing further evidence that the identified proteins are B-Raf interactors, which constitute a basis for understanding MAPK pathway regulation in neurons. © 2010 Elsevier B.V.

Registro:

Documento: Artículo
Título:Characterization of the B-Raf interactome in mouse hippocampal neuronal cells
Autor:Bonfiglio, J.J.; Maccarrone, G.; Rewerts, C.; Holsboer, F.; Arzt, E.; Turck, C.W.; Silberstein, S.
Filiación:Laboratorio de Fisiología y Biología Molecular, Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales (FCEN), Universidad de Buenos Aires, and IFIBYNE-CONICET, Buenos Aires, Argentina
Max Planck Institute of Psychiatry, Kraepelinstrasse 2-10, 80804 Munich, Germany
Palabras clave:B-Raf; Interactome; Mass spectrometry; Neurons; Proteomics; B Raf kinase; cell surface receptor; chaperone; mitogen activated protein kinase; amino acid sequence; animal cell; article; enzyme activation; enzyme regulation; gel electrophoresis; hippocampus; matrix assisted laser desorption ionization time of flight mass spectrometry; mouse; nerve cell; nonhuman; priority journal; protein analysis; protein expression; protein interaction; proteomics; RNA metabolism; RNA translation; signal transduction; Animals; Blotting, Western; Cells, Cultured; Chromatography, Liquid; Cytoskeleton; Electrophoresis, Gel, Two-Dimensional; Hippocampus; Mice; Molecular Chaperones; Neurons; Proteomics; Proto-Oncogene Proteins B-raf; RNA, Messenger; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Año:2011
Volumen:74
Número:2
Página de inicio:186
Página de fin:198
DOI: http://dx.doi.org/10.1016/j.jprot.2010.10.006
Título revista:Journal of Proteomics
Título revista abreviado:J. Proteomics
ISSN:18743919
CAS:mitogen activated protein kinase, 142243-02-5; Molecular Chaperones; Proto-Oncogene Proteins B-raf, 2.7.11.1; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18743919_v74_n2_p186_Bonfiglio

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Citas:

---------- APA ----------
Bonfiglio, J.J., Maccarrone, G., Rewerts, C., Holsboer, F., Arzt, E., Turck, C.W. & Silberstein, S. (2011) . Characterization of the B-Raf interactome in mouse hippocampal neuronal cells. Journal of Proteomics, 74(2), 186-198.
http://dx.doi.org/10.1016/j.jprot.2010.10.006
---------- CHICAGO ----------
Bonfiglio, J.J., Maccarrone, G., Rewerts, C., Holsboer, F., Arzt, E., Turck, C.W., et al. "Characterization of the B-Raf interactome in mouse hippocampal neuronal cells" . Journal of Proteomics 74, no. 2 (2011) : 186-198.
http://dx.doi.org/10.1016/j.jprot.2010.10.006
---------- MLA ----------
Bonfiglio, J.J., Maccarrone, G., Rewerts, C., Holsboer, F., Arzt, E., Turck, C.W., et al. "Characterization of the B-Raf interactome in mouse hippocampal neuronal cells" . Journal of Proteomics, vol. 74, no. 2, 2011, pp. 186-198.
http://dx.doi.org/10.1016/j.jprot.2010.10.006
---------- VANCOUVER ----------
Bonfiglio, J.J., Maccarrone, G., Rewerts, C., Holsboer, F., Arzt, E., Turck, C.W., et al. Characterization of the B-Raf interactome in mouse hippocampal neuronal cells. J. Proteomics. 2011;74(2):186-198.
http://dx.doi.org/10.1016/j.jprot.2010.10.006