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Over the last decade, two fields have dominated the attention of sub-diffraction photonics research: Plasmonics and fluorescence nanoscopy. Nanoscopy based on single-molecule localization offers a practical way to explore plasmonic interactions with nanometre resolution. However, this seemingly straightforward technique may retrieve false positional information. Here, we make use of the DNA origami technique to both control a nanometric separation between emitters and a gold nanoparticle, and as a platform for super-resolution imaging based on single-molecule localization. This enables a quantitative comparison between the position retrieved from single-molecule localization, the true position of the emitter and full-field simulations. We demonstrate that plasmonic coupling leads to shifted molecular localizations of up to 30 nm: A single-molecule mirage.


Documento: Artículo
Título:Shifting molecular localization by plasmonic coupling in a single-molecule mirage
Autor:Raab, M.; Vietz, C.; Stefani, F.D.; Acuna, G.P.; Tinnefeld, P.
Filiación:Institute for Physical and Theoretical Chemistry, Braunschweig Integrated Centre of Systems Biology (BRICS), Braunschweig University of Technology, Rebenring 56, Braunschweig, 38106, Germany
Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, Ciudad de Buenos Aires, C1425FQD, Argentina
Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Guiraldes 2620, Ciudad de Buenos Aires, C1428EAH, Argentina
Palabras clave:DNA; gold nanoparticle; nanorod; DNA; gold; molecular analysis; nanoparticle; nanotechnology; research work; Article; comparative study; computer simulation; controlled study; feedback system; field emission; finite difference frequency domain; fluorescence microscopy; image analysis; points accumulation for imaging in nanoscale topography; positron; separation technique; single molecule imaging; single molecule mirage; surface plasmon resonance; three dimensional single molecule localization nanoscopy; topography
Título revista:Nature Communications
Título revista abreviado:Nat. Commun.
CAS:DNA, 9007-49-2


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---------- APA ----------
Raab, M., Vietz, C., Stefani, F.D., Acuna, G.P. & Tinnefeld, P. (2017) . Shifting molecular localization by plasmonic coupling in a single-molecule mirage. Nature Communications, 8.
---------- CHICAGO ----------
Raab, M., Vietz, C., Stefani, F.D., Acuna, G.P., Tinnefeld, P. "Shifting molecular localization by plasmonic coupling in a single-molecule mirage" . Nature Communications 8 (2017).
---------- MLA ----------
Raab, M., Vietz, C., Stefani, F.D., Acuna, G.P., Tinnefeld, P. "Shifting molecular localization by plasmonic coupling in a single-molecule mirage" . Nature Communications, vol. 8, 2017.
---------- VANCOUVER ----------
Raab, M., Vietz, C., Stefani, F.D., Acuna, G.P., Tinnefeld, P. Shifting molecular localization by plasmonic coupling in a single-molecule mirage. Nat. Commun. 2017;8.