Artículo

Stortz, M.; Presman, D.M.; Bruno, L.; Annibale, P.; Dansey, M.V.; Burton, G.; Gratton, E.; Pecci, A.; Levi, V. "Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape" (2017) Scientific Reports. 7(1)
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Abstract:

The distribution of the transcription machinery among different sub-nuclear domains raises the question on how the architecture of the nucleus modulates the transcriptional response. Here, we used fluorescence fluctuation analyses to quantitatively explore the organization of the glucocorticoid receptor (GR) in the interphase nucleus of living cells. We found that this ligand-activated transcription factor diffuses within the nucleus and dynamically interacts with bodies enriched in the coregulator NCoA-2, DNA-dependent foci and chromatin targets. The distribution of the receptor among the nuclear compartments depends on NCoA-2 and the conformation of the receptor as assessed with synthetic ligands and GR mutants with impaired transcriptional abilities. Our results suggest that the partition of the receptor in different nuclear reservoirs ultimately regulates the concentration of receptor available for the interaction with specific targets, and thus has an impact on transcription regulation. © 2017 The Author(s).

Registro:

Documento: Artículo
Título:Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape
Autor:Stortz, M.; Presman, D.M.; Bruno, L.; Annibale, P.; Dansey, M.V.; Burton, G.; Gratton, E.; Pecci, A.; Levi, V.
Filiación:Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina
CONICET, Universidad de Buenos Aires, IFIBYNE, Buenos Aires, Argentina
Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, NIH, Bethesda, MD, United States
CONICET, Universidad de Buenos Aires, IFIBA, Buenos Aires, Argentina
Max Delbrueck Center for Molecular Medicine, Berlin, Germany
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Buenos Aires, Argentina
CONICET, Universidad de Buenos Aires, UMYMFOR, Buenos Aires, Argentina
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Orgánica, Buenos Aires, Argentina
Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, CA, United States
CONICET, Universidad de Buenos Aires, IQUIBICEN, Buenos Aires, Argentina
Palabras clave:glucocorticoid receptor; NCOA2 protein, human; nuclear receptor coactivator 2; protein binding; binding site; cell culture; cell nucleus; chromatin; gene expression regulation; genetic transcription; genetics; human; metabolism; promoter region; transcription initiation; Binding Sites; Cell Nucleus; Cells, Cultured; Chromatin; Gene Expression Regulation; Humans; Nuclear Receptor Coactivator 2; Promoter Regions, Genetic; Protein Binding; Receptors, Glucocorticoid; Transcription, Genetic; Transcriptional Activation
Año:2017
Volumen:7
Número:1
DOI: http://dx.doi.org/10.1038/s41598-017-06676-0
Título revista:Scientific Reports
Título revista abreviado:Sci. Rep.
ISSN:20452322
CAS:Chromatin; NCOA2 protein, human; Nuclear Receptor Coactivator 2; Receptors, Glucocorticoid
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20452322_v7_n1_p_Stortz

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Citas:

---------- APA ----------
Stortz, M., Presman, D.M., Bruno, L., Annibale, P., Dansey, M.V., Burton, G., Gratton, E.,..., Levi, V. (2017) . Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape. Scientific Reports, 7(1).
http://dx.doi.org/10.1038/s41598-017-06676-0
---------- CHICAGO ----------
Stortz, M., Presman, D.M., Bruno, L., Annibale, P., Dansey, M.V., Burton, G., et al. "Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape" . Scientific Reports 7, no. 1 (2017).
http://dx.doi.org/10.1038/s41598-017-06676-0
---------- MLA ----------
Stortz, M., Presman, D.M., Bruno, L., Annibale, P., Dansey, M.V., Burton, G., et al. "Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape" . Scientific Reports, vol. 7, no. 1, 2017.
http://dx.doi.org/10.1038/s41598-017-06676-0
---------- VANCOUVER ----------
Stortz, M., Presman, D.M., Bruno, L., Annibale, P., Dansey, M.V., Burton, G., et al. Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape. Sci. Rep. 2017;7(1).
http://dx.doi.org/10.1038/s41598-017-06676-0