Talarico, L.B.; Batalle, J.P.; Byrne, A.B.; Brahamian, J.M.; Ferretti, A.; García, A.G.; Mauri, A.; Simonetto, C.; Hijano, D.R.; Lawrence, A.; Acosta, P.L.; Caballero, M.T.; Paredes Rojas, Y.; Ibañez, L.I.; Melendi, G.A.; Rey, F.A.; Damonte, E.B.; Harris, E.; Polack, F.P. "The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection" (2017) EBioMedicine. 20:202-216
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Dengue is the most prevalent arthropod-borne viral disease worldwide and is caused by the four dengue virus serotypes (DENV-1-4). Sequential heterologous DENV infections can be associated with severe disease manifestations. Here, we present an immunocompetent mouse model of secondary DENV infection using non mouse-adapted DENV strains to investigate the pathogenesis of severe dengue disease. C57BL/6 mice infected sequentially with DENV-1 (strain Puerto Rico/94) and DENV-2 (strain Tonga/74) developed low platelet counts, internal hemorrhages, and increase of liver enzymes. Cross-reactive CD8+ T lymphocytes were found to be necessary and sufficient for signs of severe disease by adoptively transferring of DENV-1-immune CD8+ T lymphocytes before DENV-2 challenge. Disease signs were associated with production of tumor necrosis factor (TNF)-α and elevated cytotoxicity displayed by heterotypic anti-DENV-1 CD8+ T lymphocytes. These findings highlight the critical role of heterotypic anti-DENV CD8+ T lymphocytes in manifestations of severe dengue disease. © 2017 The Authors


Documento: Artículo
Título:The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection
Autor:Talarico, L.B.; Batalle, J.P.; Byrne, A.B.; Brahamian, J.M.; Ferretti, A.; García, A.G.; Mauri, A.; Simonetto, C.; Hijano, D.R.; Lawrence, A.; Acosta, P.L.; Caballero, M.T.; Paredes Rojas, Y.; Ibañez, L.I.; Melendi, G.A.; Rey, F.A.; Damonte, E.B.; Harris, E.; Polack, F.P.
Filiación:Fundación INFANT, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina
Department of Pediatrics, Vaccine Center, Vanderbilt University, Nashville, TN, United States
Centro de Virología Animal (CEVAN), Instituto de Ciencia y Tecnología Dr. César Milstein, CONICET, Buenos Aires, Argentina
Département de Virologie, Institut Pasteur, Unité de Virologie Structurale, Paris, France
Laboratorio de Virología, Departamento de Química Biológica-IQUIBICEN (CONICET-UBA), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Division of Infectious Diseases and Vaccinology, School of Public Health, University of California Berkeley, Berkeley, CA, United States
Palabras clave:CD8+ T lymphocytes; Dengue virus; Immune response; Immunocompetent mouse model; Secondary infection; enrofloxacin; granzyme B; ketamine; tramadol; xylazine; immunoglobulin G; neutralizing antibody; T lymphocyte receptor; virus antibody; adoptive transfer; Aedes albopictus; alanine aminotransferase blood level; alkaline phosphatase blood level; animal cell; animal model; animal tissue; antibody response; antisepsis; Article; aspartate aminotransferase blood level; bleeding time; blood vessel permeability; CD8+ T lymphocyte; controlled study; creatine kinase blood level; dengue; Dengue virus 1; Dengue virus 2; depilation; female; flow cytometry; histopathology; Human respiratory syncytial virus; humoral immune deficiency; immunoassay; lactate dehydrogenase blood level; male; mouse; nonhuman; priority journal; real time polymerase chain reaction; RNA extraction; T cell depletion; thrombocyte count; ultraviolet irradiation; virus neutralization; virus titration; wound healing; animal; CD8+ T lymphocyte; cell line; classification; cross reaction; dengue; Dengue virus; disease model; immunology; knockout mouse; lymphocyte depletion; metabolism; serotype; severity of illness index; T lymphocyte subpopulation; virology; virus load; Animals; Antibodies, Neutralizing; Antibodies, Viral; CD8-Positive T-Lymphocytes; Cell Line; Cross Reactions; Dengue; Dengue Virus; Disease Models, Animal; Immunoglobulin G; Lymphocyte Depletion; Mice; Mice, Knockout; Serogroup; Severity of Illness Index; T-Cell Antigen Receptor Specificity; T-Lymphocyte Subsets; Viral Load
Página de inicio:202
Página de fin:216
Título revista:EBioMedicine
Título revista abreviado:EBioMedicine
CAS:enrofloxacin, 93106-60-6; granzyme B; ketamine, 1867-66-9, 6740-88-1, 81771-21-3; tramadol, 27203-92-5, 36282-47-0; xylazine, 23076-35-9, 7361-61-7; immunoglobulin G, 97794-27-9; Antibodies, Neutralizing; Antibodies, Viral; Immunoglobulin G


  • Amorim, J.H., dos Santos Alves, R.P., Bizerra, R., Araujo Pereira, S., Ramos Pereira, L., Nascimento Fabris, D.L., Santos, R.A., de Souza Ferreira, L.C., Antibodies are not required to a protective immune response against dengue virus elicited in a mouse encephalitis model (2016) Virology, 487, pp. 41-49. , (pii)
  • Anantapreecha, S., Chanama, S., A-n, A., Naemkhunthot, S., Sa-Ngasang, A., Sawanpanyalert, P., Kurane, I., Serological and virological features of dengue fever and dengue haemorrhagic fever in Thailand from 1999 to 2002 (2005) Epidemiol. Infect., 133, pp. 503-507
  • Ashour, J., Morrison, J., Laurent-Rolle, M., Belicha-Villanueva, A., Plumlee, C.R., Bernal-Rubio, D., Williams, K.L., Schindler, C., Mouse STAT2 restricts early dengue virus replication (2010) Cell Host Microbe, 8, pp. 410-421. , (pii)
  • de Azeredo, E.L., Monteiro, R.Q., de-Oliveira Pinto, L.M., Thrombocytopenia in dengue: interrelationship between virus and the imbalance between coagulation and fibrinolysis and inflammatory mediators (2015) Mediat. Inflamm., 2015, p. 313842
  • Balsitis, S.J., Williams, K.L., Lachica, R., Flores, D., Kyle, J.L., Mehlhop, E., Johnson, S., Harris, E., Lethal antibody enhancement of dengue disease in mice is prevented by Fc modification (2010) PLoS Pathog., 6
  • Bashyam, H.S., Green, S., Rothman, A.L., Dengue virus-reactive CD8 + T cells display quantitative and qualitative differences in their response to variant epitopes of heterologous viral serotypes (2006) J. Immunol., 176, pp. 2817-2824. , (pii)
  • Beatty, P.R., Puerta-Guardo, H., Killingbeck, S.S., Glasner, D.R., Hopkins, K., Harris, E., Dengue virus NS1 triggers endothelial permeability and vascular leak that is prevented by NS1 vaccination (2015) Sci Transl Med, 7. , (pii)
  • Beaumier, C.M., Rothman, A.L., Cross-reactive memory CD4 + T cells alter the CD8 + T-cell response to heterologous secondary dengue virus infections in mice in a sequence-specific manner (2009) Viral Immunol., 22, pp. 215-219
  • Bhatt, S., Gething, P.W., Brady, O.J., Messina, J.P., Farlow, A.W., Moyes, C.L., Drake, J.M., Sankoh, O., The global distribution and burden of dengue (2013) Nature, 496, pp. 504-507. , (pii)
  • Blaney, J.E., Jr., Hanson, C.T., Hanley, K.A., Murphy, B.R., Whitehead, S.S., Vaccine candidates derived from a novel infectious cDNA clone of an American genotype dengue virus type 2 (2004) BMC Infect. Dis., 4 (39)
  • Blaney, J.E., Jr., Sathe, N.S., Hanson, C.T., Firestone, C.Y., Murphy, B.R., Whitehead, S.S., Vaccine candidates for dengue virus type 1 (DEN1) generated by replacement of the structural genes of rDEN4 and rDEN4Delta30 with those of DEN1 (2007) Virol. J., 4, p. 23. , (1743-422X-4-23 [pii])
  • Callahan, J.D., Wu, S.J., Dion-Schultz, A., Mangold, B.E., Peruski, L.F., Watts, D.M., Porter, K.R., King, C.C., Development and evaluation of serotype- and group-specific fluorogenic reverse transcriptase PCR (TaqMan) assays for dengue virus (2001) J. Clin. Microbiol., 39, pp. 4119-4124
  • Carvalho, D.M., Garcia, F.G., Terra, A.P., Lopes Tosta, A.C., Silva Lde, A., Castellano, L.R., Silva Teixeira, D.N., Elevated dengue virus nonstructural protein 1 serum levels and altered toll-like receptor 4 expression, nitric oxide, and tumor necrosis factor alpha production in dengue hemorrhagic fever patients (2014) J. Trop. Med., 2014, p. 901276
  • Chen, H.C., Lai, S.Y., Sung, J.M., Lee, S.H., Lin, Y.C., Wang, W.K., Chen, Y.C., Wu-Hsieh, B.A., Lymphocyte activation and hepatic cellular infiltration in immunocompetent mice infected by dengue virus (2004) J. Med. Virol., 73, pp. 419-431
  • Chen, R.F., Yang, K.D., Wang, L., Liu, J.W., Chiu, C.C., Cheng, J.T., Different clinical and laboratory manifestations between dengue haemorrhagic fever and dengue fever with bleeding tendency (2007) Trans. R Soc. Trop. Med. Hyg., 101, pp. 1106-1113. , (S0035-9203(07)00232-5 [pii])
  • Chu, H., George, S.L., Stinchcomb, D.T., Osorio, J.E., Partidos, C.D., CD8 + T cell responses in Flavivirus-naive individuals following immunization with a live-attenuated tetravalent dengue vaccine candidate (2015) J. Infect. Dis., , (jiv258 [pii])
  • Chunhakan, S., Butthep, P., Yoksan, S., Tangnararatchakit, K., Chuansumrit, A., Vascular leakage in dengue hemorrhagic fever is associated with dengue infected monocytes, monocyte activation/exhaustion, and cytokines production (2015) Int. J. Vasc. Med., 2015, p. 917143
  • De La Cruz Hernandez, S.I., Puerta-Guardo, H., Flores-Aguilar, H., Gonzalez-Mateos, S., Lopez-Martinez, I., Ortiz-Navarrete, V., Ludert, J.E., Del Angel, R.M., A strong interferon response correlates with a milder dengue clinical condition (2014) J. Clin. Virol., 60, pp. 196-199. , (pii)
  • Delgado, M.F., Coviello, S., Monsalvo, A.C., Melendi, G.A., Hernandez, J.Z., Batalle, J.P., Diaz, L., Mitzner, W., Lack of antibody affinity maturation due to poor toll-like receptor stimulation leads to enhanced respiratory syncytial virus disease (2009) Nat. Med., 15, pp. 34-41. , (pii)
  • Duangchinda, T., Dejnirattisai, W., Vasanawathana, S., Limpitikul, W., Tangthawornchaikul, N., Malasit, P., Mongkolsapaya, J., Screaton, G., Immunodominant T-cell responses to dengue virus NS3 are associated with DHF (2010) Proc. Natl. Acad. Sci. U. S. A., 107, pp. 16922-16927. , (pii)
  • Elong Ngono, A., Chen, H.W., Tang, W.W., Joo, Y., King, K., Weiskopf, D., Sidney, J., Shresta, S., Protective role of cross-reactive CD8 T cells against dengue virus infection (2016) EBioMedicine, 13, pp. 284-293. , (S2352-3964(16)30461-3 [pii])
  • Finke, D., Acha-Orbea, H., Differential migration of in vivo primed B and T lymphocytes to lymphoid and non-lymphoid organs (2001) Eur. J. Immunol., 31, pp. 2603-2611. , 10.1002/1521-4141(200109)31:9<2603::AID-IMMU2603>3.0.CO;2-8, 9&#60;2603::AID-IMMU2603&#62;3.0.CO;2-8 ([pii])
  • Franca, R.F., Zucoloto, S., da Fonseca, B.A., A BALB/c mouse model shows that liver involvement in dengue disease is immune-mediated (2010) Exp. Mol. Pathol., 89, pp. 321-326. , (pii)
  • Friberg, H., Bashyam, H., Toyosaki-Maeda, T., Potts, J.A., Greenough, T., Kalayanarooj, S., Gibbons, R.V., Green, S., Cross-reactivity and expansion of dengue-specific T cells during acute primary and secondary infections in humans (2011) Sci. Report., 1, p. 51
  • Friberg, H., Burns, L., Woda, M., Kalayanarooj, S., Endy, T.P., Stephens, H.A., Green, S., Mathew, A., Memory CD8 + T cells from naturally acquired primary dengue virus infection are highly cross-reactive (2011) Immunol. Cell Biol., 89, pp. 122-129. , (pii)
  • Fried, J.R., Gibbons, R.V., Kalayanarooj, S., Thomas, S.J., Srikiatkhachorn, A., Yoon, I.K., Jarman, R.G., Cummings, D.A., Serotype-specific differences in the risk of dengue hemorrhagic fever: an analysis of data collected in Bangkok, Thailand from 1994 to 2006 (2010) PLoS Negl. Trop. Dis., 4
  • Ghosh, A., Dar, L., Dengue vaccines: challenges, development, current status and prospects (2015) Indian J. Med. Microbiol., 33, pp. 3-15. , (doi: IndianJMedMicrobiol_2015_33_1_3_148369 [pii])
  • Gibbons, R.V., Vaughn, D.W., Dengue: an escalating problem (2002) BMJ, 324, pp. 1563-1566
  • Gruenberg, A., Woo, W.S., Biedrzycka, A., Wright, P.J., Partial nucleotide sequence and deduced amino acid sequence of the structural proteins of dengue virus type 2, New Guinea C and PUO-218 strains (1988) J. Gen. Virol., 69, pp. 1391-1398
  • Gubler, D.J., The economic burden of dengue (2012) Am.J.Trop. Med. Hyg., 86, pp. 743-744. , (pii)
  • Gubler, D.J., Reed, D., Rosen, L., Hitchcock, J.R., Jr., Epidemiologic, clinical, and virologic observations on dengue in the Kingdom of Tonga (1978) Am.J.Trop. Med. Hyg., 27, pp. 581-589
  • Guzman, M.G., Harris, E., Dengue (2015) Lancet, 385, pp. 453-465. , (pii)
  • Guzman, M.G., Kouri, G., Bravo, J., Soler, M., Martinez, E., Sequential infection as risk factor for dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS) during the 1981 dengue hemorrhagic Cuban epidemic (1991) Mem. Inst. Oswaldo Cruz, 86, p. 367. , (doi: S0074-02761991000300011 [pii])
  • Guzman, M.G., Halstead, S.B., Artsob, H., Buchy, P., Farrar, J., Gubler, D.J., Hunsperger, E., Martinez, E., Dengue: a continuing global threat (2010) Nat. Rev. Microbiol., 8, pp. S7-16. , (pii)
  • Halstead, S.B., Immune enhancement of viral infection (1982) Prog. Allergy, 31, pp. 301-364
  • Halstead, S.B., Pathogenesis of dengue: challenges to molecular biology (1988) Science, 239, pp. 476-481
  • Halstead, S.B., Neutralization and antibody-dependent enhancement of dengue viruses (2003) Adv. Virus Res., 60, pp. 421-467
  • Halstead, S.B., Lan, N.T., Myint, T.T., Shwe, T.N., Nisalak, A., Kalyanarooj, S., Nimmannitya, S., Endy, T.P., Dengue hemorrhagic fever in infants: research opportunities ignored (2002) Emerg. Infect. Dis., 8, pp. 1474-1479
  • Kalayanarooj, S., Vaughn, D.W., Nimmannitya, S., Green, S., Suntayakorn, S., Kunentrasai, N., Viramitrachai, W., Innis, B.L., Early clinical and laboratory indicators of acute dengue illness (1997) J. Infect. Dis., 176, pp. 313-321
  • Kinney, R.M., Butrapet, S., Chang, G.J., Tsuchiya, K.R., Roehrig, J.T., Bhamarapravati, N., Gubler, D.J., Construction of infectious cDNA clones for dengue 2 virus: strain 16681 and its attenuated vaccine derivative, strain PDK-53 (1997) Virology, 230, pp. 300-308. , (S0042-6822(97)98500-7 [pii])
  • Kou, Z., Quinn, M., Chen, H., Rodrigo, W.W., Rose, R.C., Schlesinger, J.J., Jin, X., Monocytes, but not T or B cells, are the principal target cells for dengue virus (DV) infection among human peripheral blood mononuclear cells (2008) J. Med. Virol., 80, pp. 134-146
  • Kouri, G.P., Guzman, M.G., Bravo, J.R., Triana, C., Dengue haemorrhagic fever/dengue shock syndrome: lessons from the Cuban epidemic, 1981 (1989) Bull. World Health Organ., 67, pp. 375-380
  • Kurane, I., Dengue hemorrhagic fever with special emphasis on immunopathogenesis (2007) Comp. Immunol. Microbiol. Infect. Dis., 30, pp. 329-340. , (S0147-9571(07)00053-7 [pii])
  • Laoprasopwattana, K., Libraty, D.H., Endy, T.P., Nisalak, A., Chunsuttiwat, S., Vaughn, D.W., Reed, G., Green, S., Dengue Virus (DV) enhancing antibody activity in preillness plasma does not predict subsequent disease severity or viremia in secondary DV infection (2005) J. Infect. Dis., 192, pp. 510-519. , (JID34061 [pii])
  • Libraty, D.H., Endy, T.P., Houng, H.S., Green, S., Kalayanarooj, S., Suntayakorn, S., Chansiriwongs, W., Ennis, F.A., Differing influences of virus burden and immune activation on disease severity in secondary dengue-3 virus infections (2002) J. Infect. Dis., 185, pp. 1213-1221. , (JID011089 [pii])
  • Lord, S.J., Rajotte, R.V., Korbutt, G.S., Bleackley, R.C., Granzyme B: a natural born killer (2003) Immunol. Rev., 193, pp. 31-38. , (doi: 044 [pii])
  • Martina, B.E., Koraka, P., Osterhaus, A.D., Dengue virus pathogenesis: an integrated view (2009) Clin. Microbiol. Rev., 22, pp. 564-581. , (pii)
  • Midgley, C.M., Bajwa-Joseph, M., Vasanawathana, S., Limpitikul, W., Wills, B., Flanagan, A., Waiyaiya, E., Chotiyarnwong, P., An in-depth analysis of original antigenic sin in dengue virus infection (2011) J. Virol., 85, pp. 410-421. , (pii)
  • Milligan, G.N., Sarathy, V.V., Infante, E., Li, L., Campbell, G.A., Beatty, P.R., Harris, E., Bourne, N., A dengue virus type 4 model of disseminated lethal infection in AG129 mice (2015) PLoS One, 10. , (pii)
  • Mongkolsapaya, J., Dejnirattisai, W., Xu, X.N., Vasanawathana, S., Tangthawornchaikul, N., Chairunsri, A., Sawasdivorn, S., Rowland-Jones, S., Original antigenic sin and apoptosis in the pathogenesis of dengue hemorrhagic fever (2003) Nat. Med., 9, pp. 921-927. , (pii)
  • Mongkolsapaya, J., Duangchinda, T., Dejnirattisai, W., Vasanawathana, S., Avirutnan, P., Jairungsri, A., Khemnu, N., Sae-Jang, K., T cell responses in dengue hemorrhagic fever: are cross-reactive T cells suboptimal? (2006) J. Immunol., 176, pp. 3821-3829. , (pii)
  • Morrison, J., Garcia-Sastre, A., STAT2 signaling and dengue virus infection (2014) JAKSTAT, 3. , (pii)
  • Nguyen, T.H., Lei, H.Y., Nguyen, T.L., Lin, Y.S., Huang, K.J., Le, B.L., Lin, C.F., Vu, T.Q., Dengue hemorrhagic fever in infants: a study of clinical and cytokine profiles (2004) J. Infect. Dis., 189, pp. 221-232. , (JID30900 [pii])
  • Nimmannitya, S., Clinical spectrum and management of dengue haemorrhagic fever (1987) Southeast Asian J. Trop. Med. Public Health, 18, pp. 392-397
  • Nisalak, A., Endy, T.P., Nimmannitya, S., Kalayanarooj, S., Thisayakorn, U., Scott, R.M., Burke, D.S., Vaughn, D.W., Serotype-specific dengue virus circulation and dengue disease in Bangkok, Thailand from 1973 to 1999 (2003) Am.J.Trop. Med. Hyg., 68, pp. 191-202
  • Noisakran, S., Onlamoon, N., Songprakhon, P., Hsiao, H.M., Chokephaibulkit, K., Perng, G.C., Cells in dengue virus infection in vivo (2010) Adv. Virol., 2010, p. 164878
  • Orozco, S., Schmid, M.A., Parameswaran, P., Lachica, R., Henn, M.R., Beatty, R., Harris, E., Characterization of a model of lethal dengue virus 2 infection in C57BL/6 mice deficient in the alpha/beta interferon receptor (2012) J. Gen. Virol., 93, pp. 2152-2157. , (pii)
  • Paes, M.V., Pinhao, A.T., Barreto, D.F., Costa, S.M., Oliveira, M.P., Nogueira, A.C., Takiya, C.M., Alves, A.M., Liver injury and viremia in mice infected with dengue-2 virus (2005) Virology, 338, pp. 236-246. , (S0042-6822(05)00270-9 [pii])
  • Pang, T., Cardosa, M.J., Guzman, M.G., Of cascades and perfect storms: the immunopathogenesis of dengue haemorrhagic fever-dengue shock syndrome (DHF/DSS) (2007) Immunol. Cell Biol., 85, pp. 43-45. , (7100008 [pii])
  • Phuong, C.X., Nhan, N.T., Kneen, R., Thuy, P.T., van Thien, C., Nga, N.T., Thuy, T.T., Wills, B., Clinical diagnosis and assessment of severity of confirmed dengue infections in Vietnamese children: is the world health organization classification system helpful? (2004) Am. J. Trop. Med. Hyg., 70, pp. 172-179. , (doi: 70/2/172 pii)
  • Pierson, T.C., Diamond, M.S., Flaviviruses (2013) Fields Virology, pp. 747-794. , D.M. Knipe P.M. Howley Philadelphia Lippincott-Raven
  • Plummer, E.M., Shresta, S., Mouse models for dengue vaccines and antivirals (2014) J. Immunol. Methods, 410, pp. 34-38. , (pii)
  • Polack, F.P., Hoffman, S.J., Crujeiras, G., Griffin, D.E., A role for nonprotective complement-fixing antibodies with low avidity for measles virus in atypical measles (2003) Nat. Med., 9, pp. 1209-1213. , (pii)
  • Prestwood, T.R., Morar, M.M., Zellweger, R.M., Miller, R., May, M.M., Yauch, L.E., Lada, S.M., Shresta, S., Gamma interferon (IFN-gamma) receptor restricts systemic dengue virus replication and prevents paralysis in IFN-alpha/beta receptor-deficient mice (2012) J. Virol., 86, pp. 12561-12570. , (pii)
  • Rico-Hesse, R., Microevolution and virulence of dengue viruses (2003) Adv. Virus Res., 59, pp. 315-341
  • Rivino, L., Kumaran, E.A., Jovanovic, V., Nadua, K., Teo, E.W., Pang, S.W., Teo, G.H., Leo, Y.S., Differential targeting of viral components by CD4 + versus CD8 + T lymphocytes in dengue virus infection (2013) J. Virol., 87, pp. 2693-2706. , (pii)
  • Rothman, A.L., T lymphocyte responses to heterologous secondary dengue virus infections (2009) Ann. N. Y. Acad. Sci., 1171, pp. E36-E41. , (pii)
  • Rothman, A.L., Cellular immunology of sequential dengue virus infection and its role in disease pathogenesis (2010) Curr. Top. Microbiol. Immunol., 338, pp. 83-98
  • Rothman, A.L., Immunity to dengue virus: a tale of original antigenic sin and tropical cytokine storms (2011) Nat. Rev. Immunol., 11, pp. 532-543. , (pii)
  • Sam, S.S., Omar, S.F., Teoh, B.T., Abd-Jamil, J., AbuBakar, S., Review of dengue hemorrhagic fever fatal cases seen among adults: a retrospective study (2013) PLoS Negl. Trop. Dis., 7. , (pii)
  • Sangkawibha, N., Rojanasuphot, S., Ahandrik, S., Viriyapongse, S., Jatanasen, S., Salitul, V., Phanthumachinda, B., Halstead, S.B., Risk factors in dengue shock syndrome: a prospective epidemiologic study in Rayong, Thailand. I. The 1980 outbreak (1984) Am. J. Epidemiol., 120, pp. 653-669
  • Schmid, M.A., Harris, E., Monocyte recruitment to the dermis and differentiation to dendritic cells increases the targets for dengue virus replication (2014) PLoS Pathog., 10. , (pii)
  • Shresta, S., Sharar, K.L., Prigozhin, D.M., Beatty, P.R., Harris, E., Murine model for dengue virus-induced lethal disease with increased vascular permeability (2006) J. Virol., 80, pp. 10208-10217. , (80/20/10208 [pii])
  • Sierra, B., Perez, A.B., Vogt, K., Garcia, G., Schmolke, K., Aguirre, E., Alvarez, M., Volk, H.D., Secondary heterologous dengue infection risk: disequilibrium between immune regulation and inflammation? (2010) Cell. Immunol., 262, pp. 134-140. , (pii)
  • Sung, J.M., Lee, C.K., Wu-Hsieh, B.A., Intrahepatic infiltrating NK and CD8 T cells cause liver cell death in different phases of dengue virus infection (2012) PLoS One, 7. , (pii)
  • Talarico, L.B., Bugna, J., Wimmenauer, V., Espinoza, M.A., Quipildor, M.O., Hijano, D.R., Beccaria, M., Martinez, D., T helper type 2 bias and type 17 suppression in primary dengue virus infection in infants and young children (2013) Trans. R. Soc. Trop. Med. Hyg., 107, pp. 411-419. , (pii)
  • Vaughn, D.W., Green, S., Kalayanarooj, S., Innis, B.L., Nimmannitya, S., Suntayakorn, S., Endy, T.P., Ennis, F.A., Dengue viremia titer, antibody response pattern, and virus serotype correlate with disease severity (2000) J. Infect. Dis., 181, pp. 2-9. , (JID990867 [pii])
  • Watanabe, S., Chan, K.W., Wang, J., Rivino, L., Lok, S.M., Vasudevan, S.G., Dengue virus infection with highly neutralizing levels of cross-reactive antibodies causes acute lethal small intestinal pathology without a high level of viremia in mice (2015) J. Virol., 89, pp. 5847-5861. , (pii)
  • Weiskopf, D., Angelo, M.A., de Azeredo, E.L., Sidney, J., Greenbaum, J.A., Fernando, A.N., Broadwater, A., de Silva, A.M., Comprehensive analysis of dengue virus-specific responses supports an HLA-linked protective role for CD8 + T cells (2013) Proc. Natl. Acad. Sci. U. S. A., 110, pp. E2046-E2053. , (pii)
  • Weiskopf, D., Bangs, D.J., Sidney, J., Kolla, R.V., De Silva, A.D., de Silva, A.M., Crotty, S., Sette, A., Dengue virus infection elicits highly polarized CX3CR1 + cytotoxic CD4 + T cells associated with protective immunity (2015) Proc. Natl. Acad. Sci. U. S. A., 112, pp. E4256-E4263. , (pii)
  • Weiss, E.J., Hamilton, J.R., Lease, K.E., Coughlin, S.R., Protection against thrombosis in mice lacking PAR3 (2002) Blood, 100, pp. 3240-3244
  • Whitehead, S.S., Falgout, B., Hanley, K.A., Blaney, J.E., Jr., Markoff, L., Murphy, B.R., A live, attenuated dengue virus type 1 vaccine candidate with a 30-nucleotide deletion in the 3′ untranslated region is highly attenuated and immunogenic in monkeys (2003) J. Virol., 77, pp. 1653-1657
  • Yacoub, S., Mongkolsapaya, J., Screaton, G., The pathogenesis of dengue (2013) Curr. Opin. Infect. Dis., 26, pp. 284-289
  • Yauch, L.E., Zellweger, R.M., Kotturi, M.F., Qutubuddin, A., Sidney, J., Peters, B., Prestwood, T.R., Shresta, S., A protective role for dengue virus-specific CD8 + T cells (2009) J. Immunol., 182, pp. 4865-4873. , (pii)
  • Yauch, L.E., Prestwood, T.R., May, M.M., Morar, M.M., Zellweger, R.M., Peters, B., Sette, A., Shresta, S., CD4 + T cells are not required for the induction of dengue virus-specific CD8 + T cell or antibody responses but contribute to protection after vaccination (2010) J. Immunol., 185, pp. 5405-5416. , (pii)
  • Zellweger, R.M., Shresta, S., Mouse models to study dengue virus immunology and pathogenesis (2014) Front. Immunol., 5, p. 151
  • Zellweger, R.M., Prestwood, T.R., Shresta, S., Enhanced infection of liver sinusoidal endothelial cells in a mouse model of antibody-induced severe dengue disease (2010) Cell Host Microbe, 7, pp. 128-139. , (pii)
  • Zellweger, R.M., Eddy, W.E., Tang, W.W., Miller, R., Shresta, S., CD8 + T cells prevent antigen-induced antibody-dependent enhancement of dengue disease in mice (2014) J. Immunol., 193, pp. 4117-4124. , (pii)
  • Zellweger, R.M., Tang, W.W., Eddy, W.E., King, K., Sanchez, M.C., Shresta, S., CD8 + T cells can mediate short-term protection against heterotypic dengue virus reinfection in mice (2015) J. Virol., 89, pp. 6494-6505. , (pii)
  • Zivna, I., Green, S., Vaughn, D.W., Kalayanarooj, S., Stephens, H.A., Chandanayingyong, D., Nisalak, A., Rothman, A.L., T cell responses to an HLA-B*07-restricted epitope on the dengue NS3 protein correlate with disease severity (2002) J. Immunol., 168, pp. 5959-5965
  • Zompi, S., Harris, E., Animal models of dengue virus infection (2012) Viruses, 4, pp. 62-82. , (pii)
  • Zompi, S., Santich, B.H., Beatty, P.R., Harris, E., Protection from secondary dengue virus infection in a mouse model reveals the role of serotype cross-reactive B and T cells (2012) J. Immunol., 188, pp. 404-416. , (pii)


---------- APA ----------
Talarico, L.B., Batalle, J.P., Byrne, A.B., Brahamian, J.M., Ferretti, A., García, A.G., Mauri, A.,..., Polack, F.P. (2017) . The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection. EBioMedicine, 20, 202-216.
---------- CHICAGO ----------
Talarico, L.B., Batalle, J.P., Byrne, A.B., Brahamian, J.M., Ferretti, A., García, A.G., et al. "The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection" . EBioMedicine 20 (2017) : 202-216.
---------- MLA ----------
Talarico, L.B., Batalle, J.P., Byrne, A.B., Brahamian, J.M., Ferretti, A., García, A.G., et al. "The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection" . EBioMedicine, vol. 20, 2017, pp. 202-216.
---------- VANCOUVER ----------
Talarico, L.B., Batalle, J.P., Byrne, A.B., Brahamian, J.M., Ferretti, A., García, A.G., et al. The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection. EBioMedicine. 2017;20:202-216.