Artículo

Czuchlej, S.C.; Volonteri, M.C.; Regueira, E.; Ceballos, N.R."Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura)" (2019) Journal of Experimental Zoology Part A: Ecological and Integrative Physiology. 331(1):17-26
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Abstract:

In rat Leydig cells, glucocorticoids (GCs) inhibit testosterone production through the interaction with the glucocorticoid receptor (GR). However, the sensitivity of those cells to GCs is regulated by the enzyme 11β-hydroxysteroid dehydrogenase Type 1 (11β-HSD1). In the testes of the toad Rhinella arenarum, the presence of an 11β-HSD similar to type 2 and a cytosolic GR has also been described. However, there is a lack of information regarding the effects of GCs on amphibian testicular steroidogenesis. In this study, the effects of corticosterone on androgen production, and the activity of two steroidogenic enzymes in toad testes were reported. Corticosterone inhibits androgen production via the GR because the GR antagonist RU486 prevents corticosterone-induced inhibition of testosterone. Corticosterone also reduced the activity of the cytochrome P450 17-hydroxylase, C17,20-lyase (Cyp450 c17) without affecting the 3β-hydroxysteroid dehydrogenase/isomerase activity. This effect on Cyp450 c17 was likewise inhibited by RU486. On the other hand, corticosterone had no effect on the amount of steroidogenic acute regulator protein. These results suggest that GCs inhibit steroidogenesis in toad testes by reducing of Cyp450 c17 activity via a GR-mediated mechanism. © 2018 Wiley Periodicals, Inc.

Registro:

Documento: Artículo
Título:Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura)
Autor:Czuchlej, S.C.; Volonteri, M.C.; Regueira, E.; Ceballos, N.R.
Filiación:Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Diversidad y Evolución Austral (IDEAus CENPAT-CONICET), Puerto Madryn, Chubut, Argentina
Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA UBA- CONICET), Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Palabras clave:amphibians; androgens; glucocorticoids (GC); steroidogenesis; testes
Año:2019
Volumen:331
Número:1
Página de inicio:17
Página de fin:26
DOI: http://dx.doi.org/10.1002/jez.2232
Handle:http://hdl.handle.net/20.500.12110/paper_24715638_v331_n1_p17_Czuchlej
Título revista:Journal of Experimental Zoology Part A: Ecological and Integrative Physiology
Título revista abreviado:J. Exp. Zool. Part A Ecol. Integr. Physiol.
ISSN:24715638
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_24715638_v331_n1_p17_Czuchlej

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Citas:

---------- APA ----------
Czuchlej, S.C., Volonteri, M.C., Regueira, E. & Ceballos, N.R. (2019) . Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura). Journal of Experimental Zoology Part A: Ecological and Integrative Physiology, 331(1), 17-26.
http://dx.doi.org/10.1002/jez.2232
---------- CHICAGO ----------
Czuchlej, S.C., Volonteri, M.C., Regueira, E., Ceballos, N.R. "Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura)" . Journal of Experimental Zoology Part A: Ecological and Integrative Physiology 331, no. 1 (2019) : 17-26.
http://dx.doi.org/10.1002/jez.2232
---------- MLA ----------
Czuchlej, S.C., Volonteri, M.C., Regueira, E., Ceballos, N.R. "Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura)" . Journal of Experimental Zoology Part A: Ecological and Integrative Physiology, vol. 331, no. 1, 2019, pp. 17-26.
http://dx.doi.org/10.1002/jez.2232
---------- VANCOUVER ----------
Czuchlej, S.C., Volonteri, M.C., Regueira, E., Ceballos, N.R. Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura). J. Exp. Zool. Part A Ecol. Integr. Physiol. 2019;331(1):17-26.
http://dx.doi.org/10.1002/jez.2232