Artículo

Baieli, M.F.; Urtasun, N.; Martinez, M.J.; Hirsch, D.B.; Pilosof, A.M.R.; Miranda, M.V.; Cascone, O.; Wolman, F.J."Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey" (2017) Biotechnology Progress. 33(1):171-180
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Abstract:

Casein glycomacropeptide (CMP) is a 64- amino acid peptide found in cheese whey, which is released after κ-casein specific cleavage by chymosin. CMP lacks aromatic amino acids, a characteristic that makes it usable as a nutritional supplement for people with phenylketonuria. CMP consists of two nonglycosylated isoforms (aCMP A and aCMP B) and its different glycosylated forms (gCMP A and gCMP B). The most predominant carbohydrate of gCMP is N-acetylneuraminic acid (sialic acid). Here, we developed a CMP purification process based on the affinity of sialic acid for wheat germ agglutinin (WGA). After formation of chitosan beads and adsorption of WGA, the agglutinin was covalently attached with glutaraldehyde. Two matrices with different WGA density were assayed for CMP adsorption. Maximum adsorption capacities were calculated according to the Langmuir model from adsorption isotherms developed at pH 7.0, being 137.0 mg/g for the matrix with the best performance. In CMP reduction from whey, maximum removal percentage was 79% (specifically 33.7% of gCMP A and B, 75.8% of aCMP A, and 93.9% of aCMP B). The CMP was recovered as an aggregate with an overall yield of 64%. Therefore, the matrices developed are promising for CMP purification from cheese whey. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:171–180, 2017. © 2016 American Institute of Chemical Engineers

Registro:

Documento: Artículo
Título:Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey
Autor:Baieli, M.F.; Urtasun, N.; Martinez, M.J.; Hirsch, D.B.; Pilosof, A.M.R.; Miranda, M.V.; Cascone, O.; Wolman, F.J.
Filiación:Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Biotecnología, Buenos Aires, Junín 956 (1113), Argentina
Instituto de Nanobiotecnología (NANOBIOTEC), UBA, CONICET, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín, Buenos Aires 956 (1113), Argentina
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Industrias, Ciudad Universitaria (1428), Buenos Aires, Argentina
Palabras clave:bovine whey; casein glycomacropeptide; chitosan; chromatography matrix; wheat germ agglutinin; Adsorption; Affinity chromatography; Amino acids; Carboxylic acids; Chitin; Chitosan; Chromatography; Mammals; Purification; Adsorption capacities; Aromatic amino acid; Bovine whey; Casein glycomacropeptide; N-acetylneuraminic acid; Nutritional supplements; Purification process; Wheat germ agglutinin; Casein; amino acid; casein; caseinomacropeptide; chitosan; n acetylneuraminic acid; peptide fragment; whey protein; adsorption; affinity chromatography; animal; bovine; chemistry; glycosylation; isolation and purification; metabolism; milk; whey; Adsorption; Amino Acids; Animals; Caseins; Cattle; Chitosan; Chromatography, Affinity; Glycosylation; Milk; N-Acetylneuraminic Acid; Peptide Fragments; Whey; Whey Proteins
Año:2017
Volumen:33
Número:1
Página de inicio:171
Página de fin:180
DOI: http://dx.doi.org/10.1002/btpr.2404
Handle:http://hdl.handle.net/20.500.12110/paper_87567938_v33_n1_p171_Baieli
Título revista:Biotechnology Progress
Título revista abreviado:Biotechnol. Prog.
ISSN:87567938
CODEN:BIPRE
CAS:amino acid, 65072-01-7; casein, 9000-71-9; chitosan, 9012-76-4; n acetylneuraminic acid, 131-48-6; Amino Acids; caseinomacropeptide; Caseins; Chitosan; N-Acetylneuraminic Acid; Peptide Fragments; Whey Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87567938_v33_n1_p171_Baieli

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Citas:

---------- APA ----------
Baieli, M.F., Urtasun, N., Martinez, M.J., Hirsch, D.B., Pilosof, A.M.R., Miranda, M.V., Cascone, O.,..., Wolman, F.J. (2017) . Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey. Biotechnology Progress, 33(1), 171-180.
http://dx.doi.org/10.1002/btpr.2404
---------- CHICAGO ----------
Baieli, M.F., Urtasun, N., Martinez, M.J., Hirsch, D.B., Pilosof, A.M.R., Miranda, M.V., et al. "Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey" . Biotechnology Progress 33, no. 1 (2017) : 171-180.
http://dx.doi.org/10.1002/btpr.2404
---------- MLA ----------
Baieli, M.F., Urtasun, N., Martinez, M.J., Hirsch, D.B., Pilosof, A.M.R., Miranda, M.V., et al. "Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey" . Biotechnology Progress, vol. 33, no. 1, 2017, pp. 171-180.
http://dx.doi.org/10.1002/btpr.2404
---------- VANCOUVER ----------
Baieli, M.F., Urtasun, N., Martinez, M.J., Hirsch, D.B., Pilosof, A.M.R., Miranda, M.V., et al. Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey. Biotechnol. Prog. 2017;33(1):171-180.
http://dx.doi.org/10.1002/btpr.2404